Collecting Fractions

Fraction Collectors are often used for fraction collection after the detector. The online interpretation of the signal can be used to automate fractionation.

Fractions can be collected for preparative use, if desired. However, in many cases, the objective is further analysis of each fraction. In either case, you can collect the fractions based on the signal. Signal-based fraction collection is quite complex, but the Fraction Collection driver provided by Chromeleon facilitates the process. In the Server Configuration program, install the Fraction Collection driver in addition to the device driver for your fraction collector.

 Tips:

In order to perform fraction collection, a Fraction or Extended Fraction Collection license must be installed.

The Fraction Collection driver also allows you to collect fractions independently of the signal, with a fixed volume at predefined retention times.

For more information about fraction collection, refer to:

 Setting Up Fraction Collection

 Enabling Fraction Collection (General Options)

 Setting the Fraction Collection Options

 Selecting the Channel for Fraction Collection (Channel Selection Options)

 Detecting the Peak Start, Peak Maximum, and Peak End (Peak Detection Options)

 Entering More Peak Detection Parameters (Peak Detection Options)

 Determining the Delay Time (Delay and Detector Offsets)

 Editing Signal-Dependent Fraction Collection Parameters

 Editing Signal-Independent Fraction Collection Parameters

 Defining the Reactions to Certain Events

 Program Example (One Detection Channel)

 Program Example (Two Detection Channels)

 AFC-3000 - Program Example for Fraction Collection

 Teledyne Isco Foxy Jr and Foxy R1 - Example for Triggers

 Fraction Collection Control via an MS

 Fraction Collection Control via an MS for Different Samples

 Checking the Fraction Collection Status on the Control Panel

 Tracking Fraction Collection in the Chromatogram

 Tracking Fraction Collection in the Report

If you select the appropriate Post-Acquisition Steps, Chromeleon can create new samples and perform fractionation automatically. This process is also known as Autopurification. Before beginning, verify that an Extended Fraction Collection license is installed. For more information, refer to  Collecting Fractions Automatically (Autopurification) and to the autopurification manual.

 Note:

The Fraction Collection Automation driver from earlier Chromeleon versions is obsolete. It is included on the Chromeleon software CD only for compatibility reasons (Server Configuration program > Add device to timebase > Manufacturers > Obsolete). Dionex recommends that you install the new Fraction Collection driver, instead (Server Configuration program > Add device to timebase > Manufacturers > Generic).