Program Example (One Detection Channel)Collecting Fractions
Program Example (One Detection Channel)
A Program for a timebase including a Fraction Collector and one detection channel might look as follows:
-0.300 Flow = 20.000
%B = 10
UV_VIS_1.Step = 0.50
UV_VIS_1.Average = On
UV_VIS_1.MaxAutoStep = 1.0
Pressure.LowerLimit = 10.00
Pressure.UpperLimit = 200.00
;********************************************************************
;*Definition of triggers for fraction collection starts here
;********************************************************************
; Definition copied from template <Timebase>\FractionCollectionTemplate!
;FracStart: Start of fractionation collection
Trigger FracStart FracStartDetected
Collect
Log SFM_A.Tray
EndTrigger
;TubeChange: The fraction is collected to another tube when the current tube is full
Trigger TubeChange FracTubeChange
Drain
SFM_A.TubePosition = FractionCollection.TubePosition
Collect
EndTrigger
;FracEnd: End of fraction collection
Trigger FracEnd FracEndDetected
MovementMode = InterruptAndEject
Drain
SFM_A.TubePosition = FractionCollection.TubePosition
EndTrigger
;********************************************************************
;*Definition of triggers for fraction collection ends here
;********************************************************************
; Maximum liquid level in a tube in ml:
PumpDevice = "Pump"
TubeMaxVolume = 10
; Maximum number of installed fraction tubes:
FractionCollection.TotalNumberInstalled = 1092
; Basic fraction collection parameters:
MaxTubesPerFraction = Unlimited
TubeWrapping = No
; Delay time between detector output and switching valve or tube:
DelayTime = 0.4
OffsetTime = 0.0
TubeChangeDuration = 2.0 [s]
ChannelEvaluation = All
; Conditions for online peak recognition at program start:
Name = "UV_VIS_1"
;Slope at peak start:
PeakStartSlope = 2.000
;Minimum signal height at peak start:
PeakStartThreshold = 10.00
;Slope after the peak maximum:
PeakMaxSlope = -4.000
;Slope at peak end:
PeakEndSlope = -4.000
;Maximum signal height at peak end:
PeakEndThreshold = 5.00
;Signal must be less than this threshold value
;before a new peak start can be recognized:
ThresholdNoPeakEnd = 200
;Baseline drift correction:
BaselineOffset = 0.000
BaselineDrift = 0.000
; Separation start with injection:
-0.100 UV.Autozero
0.000 Wait Sampler.Ready
Flow = 20.000
%B = 10
Sampler.Inject
UV_VIS_1.AcqOn
; Basic fraction collection parameters:
CollectFractions = By_Peak
; Gradient program:
Flow = 20.000
%B = 10.0
10.000 %B = 90
13.000 %B = 90
13.000 UV_VIS_1.AcqOff
CollectFractions = No
; Regeneration and equilibration phase of the gradient program:
15.000 Flow = 20.000
%B = 90
16.000 %B = 10
End
Note:
The trigger block used in this program example refers to a preparative application with a Dionex Sample and Fraction Manager (SMF). Besides, the trigger block is intended for a preparative pump.
For more program examples, refer to:
Program Example (Two Detection Channels)
Fraction Collection Control via an MS
Fraction Collection Control via an MS for Different Samples
For an overview of the fraction collection topics, refer to Collecting Fractions.