Post-Acquisition Steps
Post-acquisition steps are data reprocessing steps for extracting and smoothing data. They can be defined in the PGM Editor. (For information about the PGM Editor, refer to Control The PGM Editor.) They can be performed online, after data acquisition, or offline of the chromatogram, UV spectrum, or Mass Spectrum. The following steps are available for data reprocessing:
Arithmetic combination of channels (arithmetic combination of 2D channels - see Combining Channels via Arithmetic Operations)
Create Fraction Analysis Samples (see Collecting Fractions Automatically (Autopurification) Creating Fraction-Type Samples)
Create Purification Samples (see Collecting Fractions Automatically (Autopurification) Creating Preparation-Type Samples)
Tip:
The Create Fraction Analysis Samples and Create Purification Samples options are part of Autopurification. They are visible only if the Extended Fraction Collection license is installed and if a connection to the server exists. A connection exists if a PGM File is associated with a timebase, the server on which this timebase resides is running, and the client is connected to this server.
Extract ED channel (for extracting an ED channel - only possible if 3D_Amp data is available)
Extract MS channel (for extracting a Mass Trace - only possible if MS data is available; see Using Mass Spectrometers Extracting Mass Traces Afterward)
Extract optimum integration path (for extracting the Optimum Integration Path - only possible if a 3D field is available; see Creating and Using Spectra Libraries Selecting the Optimum Integration Path)
Extract UV channel (for extracting a UV channel - only possible if a 3D field is available; the procedure is similar to selecting the optimum integration path)
Smooth data (for chromatogram Smoothing - see Working with Chromatograms Performing Data Smoothing)
For more information, refer to How to …: Creating and Modifying Programs Adding Post-Acquisition Steps.