Autopurification

Chromeleon supports automatic fraction collection, also referred to as autopurification. In the first step, individual samples are analyzed to determine whether fractionation is appropriate. This can depend on the following criteria:

• Existence of a target compound

• Purity of the target compound

• Amount of the target compound

• Additional user-defined conditions

It is possible to define a maximum of ten target compounds. If all conditions are fulfilled, the sample is fractionated. This step, too, is chromatographically monitored. Finally, additional chromatographic analyses can be performed for the individual fractions.

This process can be automated via Post-Acquisition Steps:

Select Create Purification Samples to create the samples for purification.

Select Create Fraction Analysis Samples to create fractionated samples.

The Auto Purif. Type sample variable identifies the autopurification step.

1. Analytic = original, analytical sample

2. Preparation = preparative sample

3. Fraction = fraction or tubes generated from the preparative sample

If samples of these three sample types belong together, their Auto Purif. Ref entries in the sample list are identical.

 Tip: 

In order to perform autopurification, an Extended Fraction Collection license must be installed on your PC.

For information about how to perform autopurification, refer to  Collecting Fractions Automatically (Autopurification) and to the operating instructions for the Autopurification Systems series.