Collecting Fractions
Fraction Collection Control via an MS for Different Samples

If there are samples with different substances, the procedure described in  Fraction Collection Control via an MS has to be changed accordingly.

In this case, Mass Traces have to be extracted online, as well (see Using Mass Spectrometers  Extracting Mass Traces Online).

1. Define User-defined Columns

Define the User-defined Columns Mass1 to Mass5 and FilterIndex to FilterIndex5 as Integer columns (see Creating and Managing Files and Data  Creating User-defined Columns). The program part below checks the content of the corresponding user-defined columns during data acquisition and thus allows you to control fraction collection Triggering.

2. Modify the Program

In the example described in Fraction Collection via an MS (see above), replace the corresponding paragraph in the Program with the following program part:

; Definition of the minimum/maximum mass and of the filter index for the channels MS_01 to MS_05:

 MS_01.MinMass = sample.mass1-0.5

 MS_01.MaxMass = sample.mass1+0.5

 MS_01.FilterIndex = sample.filterindex

 MS_02.MinMass = sample.mass2-0.5

 MS_02.MaxMass = sample.mass2+0.5

 MS_02.FilterIndex = sample.filterindex2

 MS_03.MinMass = sample.mass3-0.5

 MS_03.MaxMass = sample.mass3+0.5

 MS_03.FilterIndex = sample.filterindex3

 MS_04.MinMass = sample.mass4-0.5

 MS_04.MaxMass = sample.mass4+0.5

 MS_04.FilterIndex = sample.filterindex4

 MS_05.MinMass = sample.mass5-0.5

 MS_05.MaxMass = sample.mass5+0.5

 MS_05.FilterIndex = sample.filterindex5

3. Enter the Values in Sample List

Finally, enter the corresponding values of the single samples into each column of the sample table:

In this example, the following mass traces are relevant for controlling the Fraction Collector at the corresponding samples:

 Note:

The retention times refer to the program example described in  Fraction Collection Control via an MS (see above).

For an overview of the fraction collection topics, refer to  Collecting Fractions.