Calibration Examples

The following topics provide a detailed description of the theory and practical use of the available calibration possibilities. The first examples describe the different applications:

 Introduction and Example: 1 Standard and 1 Substance

 Several Standards with Several Substances Each

 Multiple-Point Calibration Using One Single Standard

 Calibrating Using Standards of an Old Sequence

 Standard Addition

 Calibrating Unstable Substances

 No Pure Substance Available - Known Relative Extinction Coefficient

For an overview of the different calibration options for which an Internal Standard (ISTD) is used, refer to  Calibrating with an Internal Standard Substance.

The Calibration Mode determines the standard samples that are used for calibrating specific unknown samples. For an overview of the different calibration modes and the topics that provide more information, refer to  Calibration Modes for External Calibration.

For more information about validation, refer to:

 Entering the Concentration/Amount of the Validation Sample

 Validating the Calibration Curve

For a better compatibility with PeakNet 5, Chromeleon also supports  Inverting Dependent and Independent Variables.

 Note:

To apply an existing calibration to a new sequence that consists of one or several Unknown samples, set the Calibration Mode to Fixed. Always perform calibration manually (Calibrate). The Auto-Recalibrate option is not available.