General Device Commands, Chromeleon supports the following commands for the control of Dionex HPLC and IC pumps:Dionex Pumps
In addition to the General Device Commands, Chromeleon supports the following commands for the control of Dionex HPLC and IC pumps:
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Command |
Description |
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Enter the flow rate. |
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Sets the pressure limits. |
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WasteLevel.Value |
Enter the actual waste level (i.e., the liquid level in the waste container) before starting a sequence. |
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WasteLevel.UpperLimit |
Enter the upper limit for the liquid level in the waste container in [l]. If the liquid level in the waste container reaches the upper limit, the batch will be aborted. With UltiMate 3000RS, SD and BM pumps, the pump flow is stopped automatically, if the upper limit is reached (requires pump firmware version 2.21 or later). |
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Enter the warning limit for monitoring the liquid level in the waste container. Enter the limit as a percentage of the WasteLevel.UpperLimit. |
The following property relates to the commands listed above:
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Property |
Description |
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Reports the time remaining until the liquid level in the waste container reaches the upper limit (WasteLevel.UpperLimit). |
The following additional commands are available for controlling gradient pumps (DGP-3600, DP/SP, GP40/GP50, GS50, HPG-3x00, ISO-3100, LPG-3x00, P580, and P680):
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Command |
Description |
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Changes the solvent composition and determines the gradient profile. |
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Enter the solvent volume that is available when you start the sequence. |
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%A-, %B-, %C-, %D_Level.LowerLimit |
Enter the lower limit for the solvent volume in [l]. If the liquid level in the solvent reservoirs reaches the lower limit, the batch will be aborted and the related message appears. With UltiMate 3000RS, SD and BM pumps, the pump flow is stopped automatically, if the lower limit is reached (requires pump firmware version 2.21 or later). |
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Enter the warning limit for monitoring the liquid level in the solvent reservoir. Enter the limit as a percentage of the %A, %B, %C, %D_Level.LowerLimit. |
The following property relates to the commands listed above:
|
Property |
Description |
|
Reports the time remaining until the liquid level in the solvent reservoir reaches the lower limit (%A, %B, %C, %D_Level.LowerLimit). |
Commands can be selected directly (via the toolbar, menu bar, or a control) or as part of a Program (programmable button).
They enable delivery of a specific liquid volume of defined composition, as well as starting and holding the pump. The pump is automatically placed on hold as soon as the upper or lower pressure limit is exceeded. Changing the flow rate creates a Flow Gradient, while changing the solvent composition creates a % Gradient. Flow and % gradients can be realized simultaneously.
Tip:
Dionex GP40/GP50, GS50, IC20/IC25, IP20/IP25, and IS25 pumps do not deliver flow gradient ramps. Instead, changing the flow rate creates a step change; that is, flow rate changes are made immediately, not gradually over time. Refer to Practical Tips for Device Control Pump Commands for additional information.
UltiMate 3000 Pumps, DP/SP, P580, and P680 Pumps
The UltiMate 3000 system pumps, as well as the DP/SP, LPG-3x00, P580, and P680 pumps, all support automatic pre-compression control. The pumps are capable of adjusting to the compressibility of commonly used solvents. In addition, the Learn and Freeze commands allow the P580 pump to extend pre-compression control to unknown solvent types. Pre-compression control is fully automatic for the DGP-3600, DP/SP, HPG-3x00, LPG-3x00, and P680 pumps; therefore, the Learn and Freeze commands are not required.
Command input is identical for the Low-Pressure Gradient System and High-Pressure Gradient System.
Caution:
Chromatography pumps are high-precision instruments! Dry operation or crystallization of buffer solutions within the fluidic system must be avoided. The most common cause of such problems is stopping pump flow without turning off the lamp in an optical detector. The flow cell heats up, and the solvent evaporates. Deposits of substances can result, for example, from the presence of salt in a buffer solution.
For information about special commands for the pumps, refer to
Dionex UltiMate 3000 Series NCP-3200RS Pumps
Dionex UltiMate 3000 Series NCS-3500RS Pumps
Dionex UltiMate 3000 Series BM, BX, RS and SD Pumps
Dionex UltiMate 3000 Series Pumps
Dionex DP/SP IC Pumps
Dionex P680 HPLC Pump
GP40/GP50, GS50, IC20/IC25, IP20/IP25, and IS25 Pumps
In addition to the general pump commands, the following commands are available for controlling the isocratic or gradient pumps listed above:
|
Command |
Description |
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On |
Turns the pump motor on |
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Off |
Turns the pump motor off |
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Prime |
Primes the pump |
For pump operating specifications, refer to the individual pump operator's manuals.
Also, refer to:
Dionex/LC Packings UltiMate Capillary/Nano HPLC Pump
PP-150 Preparative HPLC Pump
Controlling Pumps Without a Separate Device Driver