Dionex Pumps
Dionex UltiMate 3000 Series Pumps

In addition to the standard pump commands (see  Dionex Pumps), the UltiMate 3000 pumps support the following commands and properties (please note that the display Filter level determines which commands and properties are displayed):

 Tip:  

For information about the commands and properties that are available for the BM, BX, RS and SD pumps in the UltiMate 3000 pump series, refer to UltiMate 3000 Series BM / BX / RS and SD Pumps.

Flow and Pressure commands and properties (no split flow)

If the pump flow is not split, Flow and Pressure refer directly to the pump outlet. In the standard configuration, this is the LoadingPump, which is the left pump. The available range depends on the pump type.

 

Command/Property

Description

%Compression

Reports the current pump compression in [%] (read-only).

 Tip:  

The compression should always be less than 100%. A compression of 100% has a negative effect on the pump pulsation. Also, a high pump compression indicates that the eluent is not properly degassed or that air bubbles have occurred in the pump heads, or that the pump's working cylinder leaks.

Flow

Sets the flow rate [µl/min]. This is the total flow through the pump.

MaximumFlowRampDown

Sets the upper limit for the flow rate deceleration.

MaximumFlowRampUp

Sets the upper limit for the flow rate acceleration.

Pressure

Reports the pressure. (This property is read-only. The channel is recorded as LoadingPump_Pressure.)

*.LowerLimit
*.UpperLimit

Sets the lower and upper pressure limits at the loading pump. If the pressure exceeds the upper limit for 0.15 seconds or if it is below the lower limit for more than 60 seconds, the system aborts the running batch and starts error handling (see Pressure.Lower/UpperLimit).

 

Pump_Pressure

If you select the Pressure Signal(s) check box on the Devices page in the Server Configuration program, Chromeleon records the pump pressure as a separate channel (Pump_Pressure). The following commands and properties are available:

 

AcqOn/AcqOff

AcqOn starts data acquisition. AcqOff terminates data acquisition.

Average

Averages all measured values.

Delta

Reports the signal's slope, i.e., the difference between the value and the value 1 second ago (read-only). This is useful for Triggers.

MaxAutoStep

Sets the maximum step rate for Step = Auto.

Retention

Indicates the retention time for the signal.

Signal

Reports the current pressure signal.

Step

Sets the step for data acquisition.

Flow and Pressure commands and properties (micro pumps with split flow)

If the pump is connected to a flow splitter, Flow and Pressure refer to the column flow and column pressure. (In the standard configuration, Dionex recommends assigning the flow splitter to the right pump (MicroPump).) The values for the flow and pressure at the pump outlet are MasterFlow and MasterPressure. The available range of any properties depends on the pump type and the flow splitter's split ratio.

Command/Property

Description

Flow

Sets the flow rate [µl/min]. This is the total flow through the column. (The valid range depends on the split ratio.)

MasterFlow

Reports the flow through the master pump, i.e., the flow before the flow splitter, in [µl/min].

MasterPressure

Reports the pressure of the master pump, i.e., the pressure before the flow splitter. (This property is read-only. The channel is recorded as MicroPumpMasterPressure.)

*.LowerLimit
*.UpperLimit

Sets the lower and upper pressure limits at the master pump. If the pressure exceeds the upper limit for 0.15 seconds or if it is below the lower limit for more than 60 seconds, the system aborts the running batch and starts error handling (see Pressure.Lower/UpperLimit).

MaximumFlowRampDown

Sets the upper limit for the flow rate deceleration (column flow).

MaximumFlowRampUp

Sets the upper limit for the flow rate acceleration (column flow).

Pressure

Reports the current column pressure. (This property is read-only. The channel is recorded as ColumnPressure.)

*.LowerLimit
*.UpperLimit

Sets the lower and upper pressure limits at the column. If the pressure exceeds the upper limit or if it is below the lower limit for more than 5 minutes, the system aborts the running batch and starts error handling.

 

 

Display commands and properties

Command/Property

Range/Values

Description

Brightness

0 - 100 %

Adapts the brightness of the pump display to your requirements.

Contrast

0 - 100 %

Adapts the screen contrast of the pump display to your requirements.

DisplayMode

(only for micro pumps with split flow)

Master
Column

Specifies whether the pump display shows the flow and pressure values at the column (Column) or at the pump outlet (Master).

 

Additional commands and properties

Command/Property

Range/Value

Description

InstalledOn

 

Indicates the timebase in which the pump is installed.

MixingChamber

Unknown
None
14µl_Micro
328µl_Analytical
600µl_SemiPreparative
1200µl_ExtendedSemiPrep

Enter the mixing chamber volume.

ParkPercentage

(only for micro pumps)

0.00 - 100.00 %

Enables peak parking. The flow is reduced to the specified percentage of the current flow.

PeakParked

(only for micro pumps)

 

No,
Yes

Reports the status of peak parking (read-only). Reports Yes while the pump delivers the reduced flow set by ParkPercentage.

Purge

On,
Off

Set to On to enable purging of the pump. Also, see PurgeTime and PurgeFlow.

PurgeFlow

 

Enter the flow [in mL/min] to be delivered by the pump during purging. Also, see Purge and PurgeTime.

PurgeTime

 

Specify how long purging shall be performed [s]. Also, see Purge and PurgeFlow.

PurgeViaSampler**

Yes,
No

Set to Yes to enable purging of the pump via the autosampler. See also Purge and SamplerDevice.

 Note:

This function is available only if your UltiMate 3000 system includes a WPS-3000SL or WPS-3000RS autosampler.

RearSealWashPump

Idle,
Active

Indicates whether the peristaltic pump of the rear seal wash system is running or not (read-only).

RearSealWashSystem

 

Turns the rear seal washing on (Interval or Automatic) or off.
Interval activates rear-seal washing once per interval (see RearSealWashInterval) for five minutes. However, the drop sensor on the liquid reservoir is not active, i.e., piston seal monitoring is disabled.
Automatic periodically activates rear-seal washing once per interval until the drop sensor has counted 30 drops. The drop sensor is active, i.e., piston seal monitoring is enabled.
Off turns the rear-seal wash system off.

RearSealWashInterval

 

Specifies the time interval [min] when the next rear seal wash is performed.

Relay2Enabled

Yes (0),
No (1)

Determines whether relay 2 can be used in Chromeleon (Yes) or whether it is controlled by the pump for the Left Cam Sync Out signal (No).

Relay3Enabled

Yes (0),
No (1)

Determines whether relay 3 can be used in Chromeleon (Yes) or whether it is controlled by the pump for the Operable Out signal (No).

Relay4Enabled

Yes (0),
No (1)

Determines whether relay 4 can be used in Chromeleon (Yes) or whether it is controlled by the pump for the Right Cam Sync Out signal (No).

SamplerDevice

 

Indicates the name of the autosampler that is used to purge the pump. See also Purge, PurgeViaSampler**.

SelfTest

Passed or
NotPassed

Performs a self-test.

 Note:

You cannot start a batch when the pump failed the self-test.

SelfTestPassed

Yes,
No

Reports whether the pump has passed the self-test (read-only). See also SelfTest.

Standby

NoStandby,
Standby

Sets the pump into the Standby mode or cancels this mode (NoStandby). In standby mode, the pump remains connected to Chromeleon.

 Note:

You cannot start a batch when the pump is in standby mode.

 

** For patent reasons, this function must not be used in the following countries: China, Germany, Great Britain, Japan, and the USA.

Equilibration commands and properties

Use the equilibration commands and properties only for the SmartStartup feature.

 

Command/Property

Description

Equilibration

Reports the equilibration status.

Ripple

Reports the ripple of the pump pressure.

UpperLimit

Determines the upper limit for the ripple of the pressure signal in [%]. The upper limit can be any value between 1.0% and 5.0%. The default setting is 3.0%.

Value

Reports the actual value. The value is calculated as soon as at least a one-minute segment was measured. It is updated with each new recorded segment and averaged over the last 5 minutes.

If the average pressure is below 10 bar, the ripple measurement is started again.

The value of the property does not change after the measurement and is displayed under Preconditions.

RippleStatus

Reports the ripple equilibration status of the pump pressure (read-only).

N/A

Equilibration has not started.

Measuring

Equilibration is running, but the amount of data is not yet sufficient to report a result.

Good

Equilibration is running or has finished. The values are within the specified limits.

NotReady

Equilibration is running. The current data is outside the specified limits.

Failed

Equilibration is completed. The result is outside the specified limits.

NotTested

The limit was set to 0, thus disabling ripple checking.

StartEquilibration

Starts equilibration (ripple measurement). For more information, refer to How to ...:  Equilibrating the Chromatography System.

 

System Wellness and Predictive Performance commands and properties

 Tip:  

The Chromeleon version and pump type determine which commands and properties are available for checking the system wellness and performance of the pump.

Command/Property

Description

AcousticAlarm

 

When enabled, a beep alerts you when the pressure is below the lower limit or exceeds the upper limit.

AnalogOut

Determines whether the pressure of the left or right pump is monitored via the Analog Out port on the pump.

ChangePistonsLeft
ChangePistonsRight

Select this command when the pistons of the related pump block needs to be changed. The pistons of the related pump block are moved into the appropriate position for the replacement and the pump stops. After the piston was changed, use the Flow command to re-start the pump.

CheckValveServiceLeft/
Right

Reports service information about the related check valve.

*.LastDate

Reports the date when the check valve was serviced last (read-only).

*.Interval

Specifies the interval (in days) when the next service for the check valve is due.

*.WarningPeriod

Specifies the period during which a warning appears before the next service is due.

CheckValveServiceDone
Left/Right

After you have replaced the related check valve, perform this command to update the related service information.

ClearDisplayError

Clears any error messages on the pumps display.

Curve

Determines the curvature for flow gradients (see Gradient Curves).

Degasser

Turns the degasser On or Off (LPG pump or SRD-3x00 only). The integrated degasser should always be on.

DegasserVacuum

Reports the status of the degasser vacuum (SRD-3x00 only): OK or NotOK (read-only).

HeadType

Indicates the head type of the pump: Analytical or Semi-Prep (read-only).

Leak

Indicates the status of the leak sensor: OK or Leak (read-only).

LeakAlarm

On indicates that there is a leak alarm (read-only).

LeakAlarmOff

Turns off the acoustic beep for the current alarm.

LeakDelay

Specifies how much time elapses between the detection of a leak and the pump shutdown.

LeakSensorMode

Specifies how leak detection is performed:
Enabled-Enables leak detection; a beep alerts you when the leak sensor reports a leak.
Silent-Enables leak detection; but no beep alerts you when the leak sensor reports a leak.
Disabled-Disables leak detection.

ModelVariant

Indicates the variant: Isocratic, LPG, or HPG (read-only).

Motor

Indicates whether the pump is delivering: On or Off (read-only).

OperatingHours

Counts the operating hours since initial operation (excluding the operating hours in standby mode; read-only).

OutletFritChangeLeft/
Right

Provides service information about the filter frits in the related outlet unit.

*.LastDate

Reports the date when the frit was replaced last (read-only).

*.Interval

Specifies the interval (in days) after which a service of the outlet frit is due.

*.WarningPeriod

Specifies the period during which a warning appears before the next service is due.

OutletFritChangedLeft/
Right

After you have replaced the outlet frit, perform this command to update the related service information.

PistonsChangedLeft/Right

After you have replaced a piston, perform this command to update the related service information.

PistonChangeDateLeft/
Right

Reports the date when the piston was changed last (read-only).

PistonWorkLoadLeft/Right

Provides information about the piston work load.

*.Value

Indicates the current work load [MJ] of the related piston (read-only).

*.Warning

A warning appears when the next service is due within the specified period.

*.Limit

An error message appears when the limit is reached.

PowerOnTime

Counts the time since last power-up (or last wake-up from standby) (read-only).

ProportioningValve_A/B/
C/D_CyclesLeft/Right

Counts how many times the related valve was switched (read-only).

PumpStrokesLeft/Right

Counts the number of piston strokes in the related pump block (read-only).

Qualification

Provides information about system qualification.

*.LastDate

Reports the date when system qualification was performed (read-only).

*.LastOperator

Reports the name of the person who performed the system qualification (read-only).

*.Interval

Reports the interval (in days) when the next system qualification is due.

*.WarningPeriod

Specifies the period during which a warning appears before the next service is due.

*.GracePeriod

Indicates the period during which the device may be operated after the due date for re-qualification.

QualificationDone

Perform this command after qualification to update the related system data.

RearSealLeakage

Provides information about pump leakage.

*.Value

Reports how many drops passed the main piston seal in the past hour (read-only).

*.Warning

A warning appears when the specified value is reached.

*.Limit

An error message when the specified value is reached.

RearSealWashPump
Hours

Counts the operating hours of the peristaltic pump (read-only).

RearSealWashTube
Changed

Perform this command to update the related service data after you have replaced the wash tubing.

RearSealWashTube
ChangeDate

Reports when the wash tubing was replaced last (read-only).

RearSealWashTubeHours

Provides information about the operating time [h] of the peristaltic pump with the currently installed wash tube.

*.Value

Reports the current operating time [h]of the peristaltic pump with the currently installed wash tube (read-only).

*.Warning

A warning appears when the specified value is reached.

*.Limit

An error message appears when the specified value is reached.

SealChangeDate
Left/Right

Reports when the related seal was replaced last (read-only).

SealWorkLoad
Left/Right

Counts the work load of the related rear seal since the seal was replaced last.

*.Value

Reports the current work load of the rear seal since the seal was replaced last (read-only).

*.Warning

A warning appears when the specified value is reached.

*.Limit

An error message appears when the specified value is reached.

SealsChangedLeft/Right

After you have replaced the seals of the related pump block, perform this command to update the related service information.

Service

Provides service information.

*.LastDate

Reports the date of the last service (read-only).

*.Interval

Specify the interval (in days) after which a service is due.

*.WarningPeriod

Specifies the period during which a warning appears before the next service is due.

ServiceDone

After the device has been serviced, perform this command to update the related information.

SetFactoryDefaults

Resets the pump settings to the Dionex factory default settings.

SolventRackLeak

Reports whether the leak sensor in the solvent rack detected a leak: Leak or NoLeak (read-only).

StandbyHours

Counts the total operating hours in standby mode since the module was operated for the first time (read-only).

WorkLoadSinceLast
ServiceLeft/Right

Counts the work load [MJ] of the related pump block since the last service (read-only).

WorkLoadLeft/Right

Reports the total work load [MJ] of the related pump block since initial operation of the module (read-only).