Dionex Nano Cap Systems
Dionex UltiMate 3000 Series NCP-3200RS and NCS-3500RS: NC Pump

In addition to the  General Device Commands and the  Dionex Pump Commands, the NC pump supports the following commands and properties (please note that the display Filter level determines which commands and properties are displayed):

Commands and properties of the main device ("PumpModule"):

General commands/properties

Command/Property

Description

Alarm

Reports whether a leak alarm or pressure alarm is pending. Default value: Off.

AlarmOff

Perform this command to reset a pending alarm. After the command has been performed, the flow can be restarted and the alarm sound turns off.

Brightness

Adapts the brightness of the pump display to your requirements.

ClearDisplayError

Clears any error messages on the pumps display.

Contrast

Adapts the screen contrast of the pump display to your requirements.

Degasser

Turns the degasser of an SRD 3x00 On or Off; default value: ON.

DegasserVacuum

Reports the status of the degasser vacuum: OK or NotOK.

Leak

Indicates the status of the leak sensor in the pump.  If the leak sensor reports a leak, a beep sounds. If the alarm continues for at least 3 minutes, the pump flow is stopped.

Note: To temporarily turn off the beep and restart the pump flow, perform the AlarmOff command. However, if the leak is not eliminated within 30 minutes, another beep sounds and the pump flow is stopped again.

LeakSensorMode

Enables or disables leak detection (default: Enabled):
Enabled (
recommended): Activates leak detection. A beep alerts you when the leak sensor reports a leak.  
Disabled:
Deactivates leak detection. The leak sensor status will be written to the Audit trail but no message appears and no beep alerts you.

ModelVariant

Reports the pump variant (Isocratic, LPG, HPG, DGP, DP or Unknown).

OverrideRearSealDry

Select Enabled to start a batch or set the pump flow even if RearSealWashStatus reports Dry. The property is automatically reset to Disabled after each wash cycle and upon each connect in Chromeleon.

RearSealWashPump

Indicates whether the peristaltic pump of the rear seal wash system is running. To stop a wash cycle, set RearSealWashPump to Idle. To start a wash run, set RearSealWashPump to Active.

Note: You can perform this command only for a single run. It is not possible to disable rear seal washing permanently.

RearSealWashStatus

Reports the status of the rear seal wash system.

Dry - the system has run out of liquid.  Also, see OverrideRearSealDry.

Operational - the system operates properly.

SetFactoryDefaults

Resets the pump settings to the Dionex factory default settings.

SolventRackLeak

Indicates the status of the leak sensor in the solvent rack that is connected to the NCS.

Standby

Displays the current operation mode.

 

 

Note:

The Degasser properties are available only for LPG-3400 pumps and pumps to which an SRD-3x00 Solvent Rack is connected. In the Server Configuration program, verify in the Properties dialog for the pump (Devices tab page) that Degasser Control is set to Internal (if the pump is an LPG-3400) or to External (if an SRD-3x00 Solvent Rack is connected to the pump).

Commands and properties for the NC flow unit ("NC_Pump"):

Command/Property

Description

%A, %B

Indicates the partial flow of a solvent component, expressed as a percentage of the total flow.

*Value

Current value [%] for components A or B.

*Equate

Name of the component (A or B). Note: Default component names can be specified in the Properties dialog (Solvents (NC Pump) tab page) for the pump in the Server Configuration program.

%A_Viscosity

%B_Viscosity

(Available only if flowmeter type Classic is used.)Specifies the viscosity of the solvent to be used with the NC pump. The viscosities of the most common solvents and solutions are predefined in Chromeleon. To use a predefined value, click the arrow next to the input box and select a value from the viscosity list.

If the value for the solvent that you are using is not provided in the list, you can do the following:

  • If the viscosity is unknown: establish the viscosity for the respective solvent and save the value (for details, refer to Viscosity Measurement). This will also update the values for %A_Viscosity and %B_Viscosity shown in the input fields.

  • The viscosity is known (the value has already been established in an earlier viscosity measurement in Chromeleon): type the value in the input box.

Please note that values entered here will be kept only until you establish and save a new value or enter a different value in the input box.

Tip:

If you change solvents frequently, you can add the viscosity to the viscosity list in the Server Configuration program. For details, refer to Editing the Viscosity List.

Notes:

The viscosity values are relative and refer to water at 42 °C.

It is not possible to calculate the viscosity of premixed solvents from the viscosities and mixing ratio of the components.

%A_Solvent

%B_Solvent

(Available only if flowmeter type ProFlow is used.)Specifies the solvent to be used with the NC pump. The 5 most used solvents are pre-calibrated in Chromeleon as well as the solvents you stored in the solvent list. To use a predefined solvent, click the arrow in the %A_Solvent or %B_Solvent box and select a value from the list.

For details on modifying list entries refer to Calibrating Solvents (NCP-3200RS/NCS-3500RS).

CurrentFlow

Displays the current flow of the flowmeter block.

Curve

Determines the curvature for flow gradients (see Gradient Curves).

Flow

Sets the flow rate [µl/min]. This is the total flow through the pump.

*Nominal

Sets the nominal flow.

*Value

Reports the current flow.

FlowMode

Reports the current flow mode of the pump (Stopped, Running, Hold, Purge, Park).

FlowMeterSerialNo

Reports the serial number of the flow meter.

FlowSelectorSerialNo

Reports the serial number of the flow selector.
(Classic flowmeter type only)

HeadType

Indicates the head type of the pump.

MaximumFlowRampDown

Sets the upper limit for the flow rate deceleration.

MaximumFlowRampUp

Sets the upper limit for the flow rate acceleration.

ModelNo

Reports the model number of the NC pump device.

ModelVariant

Reports the model variant of the NC pump device.

Motor

Turns the motors of the pump on or off.

Pressure

Reports the pressure.

*Value

Reports the current pump pressure.

*.LowerLimit
 

Sets the lower pressure limit. The pump will stop the flow if the pressure is below the lower limit.

Note: If you want to use the new limit as standard, consider changing the value in the Properties dialog (Limits tab page) for the pump in the Server Configuration program . If you want to change the limit only for a specific application, change the value here. This will not change the standard setting on the Limits page.

*.UpperLimit

Sets the upper pressure limit. The pump will stop the flow if the pressure exceeds the specified value.

Also, see Pressure.LowerLimit.

PrimPressureLeftNanoBlock

PrimPressureRightNanoBlock

Indicates the primary pressure of the right or left NC pump head.

Purge

Set Purge to On to start a purge cycle for the pump. The pump is purged with the PurgeTime. When the specified PurgeTime has elapsed, Purge is automatically reset to Off. You can stop purging also manually by setting Purge to Off.

PurgeTime

 

Specifies how long purging shall be performed [min]. For the pump head(s), a purge time of 30 minutes is recommended. Also, see Purge.

PurgeWhat

Specify the component to be purged: left or right pump head, both pump heads, or the flowmeter.

Ready

Reports whether the device is ready to operate (Ready or NotReady).

Temperature

Reports the current temperature of the NC flow control unit.

 

Pressure commands and properties ("NC_Pump_Pressure" (flowmeter type Classic) or  "NC_Pump_Press_LeftBlk" and "NC_Pump_Press_RightBlk"  as well as "NC_Pump_Flow_LeftBlk" and "NC_Pump_RightBlk" (flowmeter type ProFlow))

If you select the related pump pressure check box in the Properties dialog for the pump (Signals tab page) in the Server Configuration program, Chromeleon records the pump pressure as a separate channel. If a problem occurs, the pump pressure channel can provide helpful information to identify and eliminate the source for the problem. Therefore, you should always record the pump pressure in a program file. The following commands and properties are available:

 

AcqOn/AcqOff

AcqOn starts data acquisition. AcqOff terminates data acquisition.

Average

Averages all measured values.

Delta

Reports the signal slope, that is, the difference between the value and the value 1 second ago. This is useful for Triggers.

Equilibration

Reports the equilibration status.

MaxAutoStep

Sets the maximum step rate for Step = Auto.

Retention

Indicates the retention time for the signal.

Ripple

Reports the ripple of the pump pressure.

*Value

Reports the actual value. The value is calculated as soon as at least a one-minute segment was measured. It is updated with each new recorded segment and averaged over the last 5 minutes.

If the average pressure is below 10 bar, the ripple measurement is started again.

The value of the property does not change after the measurement and is displayed under Preconditions.

*UpperLimit

Determines the upper limit for the ripple of the pressure signal in [%]. The upper limit can be any value between 1.0% and 5.0%. The default setting is 3.0%.

RippleStatus

Reports the ripple equilibration status of the pump pressure (read-only).

N/A – Equilibration has not started.

Measuring – Equilibration is running, but the amount of data is not yet sufficient to report a result.

Good – Equilibration is running or has finished. The values are within the specified limits.

NotReady – Equilibration is running. The current data is outside the specified limits.

Failed – Equilibration is completed. The result is outside the specified limits.

NotTested – The limit was set to 0, thus disabling ripple checking.

Signal

Reports the current pressure signal.

StartEquilibration

Starts equilibration. For more information, refer to How to ...:  Equilibrating the Chromatography System.

Step

Sets the step for data acquisition.

 

System Wellness and Predictive Performance commands and properties

"PumpModule_Wellness"

Command/Property

Description

OperatingHours

Counts the operating hours since initial operation (excluding the operating hours in standby mode).

PowerOnTime

Counts the time since last power-up (or last wake-up from standby).

Qualification

Provides information about system qualification.

*.LastDate

Reports the date when the most recent qualification was performed.

*.LastOperator

Reports the name of the person who performed qualification.

*.Interval

Reports the interval (in days) when the next system qualification is due.

*.WarningPeriod

Specifies the period during which a warning appears before the next qualification is due.

*.GracePeriod

Indicates the period during which the device may be operated after the due date for re-qualification.

QualificationDone

Perform this command after qualification to update the related system data.

RearSealWashPump
Hours

Counts the operating hours of the peristaltic pump.

RearSealWashTube
Changed

Perform this command to update the related service data after you have installed new peristaltic tubing.

RearSealWashTube
ChangeDate

Reports the date when the peristaltic tubing was replaced last.

RearSealWashTubeHours

Provides information about the operating time [h] of the peristaltic pump with the currently installed tubing.

*.Value

Reports the current operating time [h] of the peristaltic pump with the currently installed tubing.

*.Warning

A warning appears when the specified value is reached.

*.Limit

An error message appears when the specified value is reached.

Service

Provides service information.

*.LastDate

 

Reports the date of the last service.

*.Interval

 

Specify the interval (in days) after which a service is due.

*.WarningPeriod

Specifies the period during which a warning appears before the next service is due.

ServiceDone

After the device has been serviced, perform this command to update the related information.

StandbyHours

 

Counts the total operating hours in standby mode since the module was operated for the first time.

 

"NC_Pump_Wellness"

Command/Property

Description

SelfTest

Perform this command to start a self-test for the pump.

Note: You cannot start a second test while the first test is still running.

SelfTestPassed

Reports whether the pump passed the test.

Note: You can start a batch only if the test was successful. If the test failed, check the pump and repeat the test.

 

"NC_Pump_Wellness_LeftBlock" and "NC_Pump_Wellness_RightBlock"

Command/Property

Description

CalibrateWP

Calibrates the working piston pressure sensor.

This command is required especially after replacement of the pump head. For detailed instructions on changing the pump head, please refer to the operating manual of the instrument

CurrentFlow

Displays the current flow of the flowmeter block.

CurrentSolvent

Displays the name of the currently selected solvent.

DeleteSolventIndex

(Available only if flowmeter type Classic is used.)

Specify the index of the entry in the solvent list that should be deleted with the next DeleteProFlowSolventEntry command.

Note: You can not delete index entries 1-5.

DeleteProFlowSolventEntry

(Available only if flowmeter type Classic is used.)

Deletes an entry from the solvent calibration list. The entry to be deleted must be specified before in DeleteSolventIndex.

DockPistons

Perform this command for the pump head for which you have replaced the pistons or piston seals. The command returns the pistons into the appropriate position for normal operation.

Wait until the PistonPositionStatus property reports Operational before you resume operation.

UndockPistons

Moves the pistons of the pump head for which you perform the command into the appropriate position for piston or piston seal replacement.

Wait until the PistonPositionStatus property reports Undocked before you start the replacement procedure.

PistonPositionStatus

Reports the position of the pistons in the pump head (also, refer to DockPistons and UndockPistons):

ErrorUndock/ErrorDock: Indicates a problem during execution of the UndockPistons or DockPistons command, respectively.

Operational: The pistons are in the appropriate position for  normal operation.

PrepareUndocking: The pistons are moving into the appropriate position for piston and piston seal replacement. During this time, it is not possible to start the flow or run a batch.

PrepareDocking: The pistons are returning to the appropriate position for normal operation after they were undocked for piston or piston seal replacement. During this time, it is not possible to start the flow or run a batch.

Undocked: The pistons are in the appropriate position for piston or piston seal replacement.

Unknown: The current position of the pistons is unknown.

 

For information on commands and properties for controlling the other modules of the NCS-3500RS, refer to:

UltiMate 3000 Series NCS-3500RS: Loading Pump

UltiMate 3000 Series NCS-3500RS System: Column Compartment

UltiMate 3000 Series NCS-3500RS: Column ID Properties

 

For information about defining customized viscosity values for the NC pump, refer to:

Viscosity Measurement (NC pumps with Classic flowmeter).

Calibrating Solvents (NCP-3200RS/NCS-3500RS) (NC pump with ProFlow flowmeter)

For information about the commands and properties that are available for other pumps in the UltiMate 3000 pump series, refer to:

UltiMate 3000 Series Pumps.