Command/Property

Description

AcquireExclusiveAccess

Acquires exclusive access for the current timebase.

AirPump

Turns the air pump on or off. The air pump provides outside air that is used to dry the needle.

AutoTrayShakeTimes

Specifies how often the tray is shaken before the sample is drawn. Tray shakes allow homogenization of the sample, especially after longer idle times.

BlueTray

Specifies which sample containers are installed in the blue segment.

Note: If you later install different sample containers in the carousel, you must update the settings:

• If you want to use the sample containers as standard containers, you can change the standard settings in the Properties dialog box for the autosampler (Segments/Pump Link tab page) in the Server Configuration program.

• If you want to use the sampler containers only for a specific application, change the settings here or on the control panel for the autosampler. Changing the settings here will not change the standard settings in the Properties dialog box.

If the settings in Chromeleon do not match the installed sample containers, the needle may be damaged.

BootLoaderVersion

Indicates the bootloader version (read-only).

Brightness

Adapts the brightness of the autosampler's front panel display to your requirements.

BufferTubingVolume

Reports the volume of the installed buffer tubing (read-only).

Contrast

Adapts the screen contrast of the autosampler's front panel display to your requirements.

DeepWellAdapterHeight

Defines the height of the adapter to be used exclusively with deep well plates (24, 96 and 384 positions) of different heights (20 - 46 mm).

 Note:

The property can only be set if the latest firmware is installed on the sampler.

DispenseDelay

Sets the time that the needle shall remain in the sample container after dispensing.

DispSpeed

Sets the speed at which the sample is expelled for standard injections

DrawDelay

Sets the time that the needle shall remain in the sample container after sample was drawn.

DrawSpeed

Sets the speed at which the sample is drawn for standard injections.

EnableTrayTest

Enables or disables automatic tray detection. By default, the tray detection is Enabled for the WPS-3000SL/RS/TXRS and Disabled for the WPS-3000PL (RS)/FC.

ExclusiveAccess

Reports which timebase has exclusive access to the autosampler (read-only).

FillWashPort

Fills the wash port with wash liquid.

FirstTransportVial

Sets the position for the first transport vial. Transport vials are required only for microliter pick-up injections. (Also, see LastTransportVial.)

FlushOutFactor

The “flush out” factor is a multiple of the sample volume and defines the number of sample volumes to be flushed out of the sample loop before the injection valve is switched from the “Inject” to “Load” position.

Enter the n-fold of the injection volume in the FlushOutFactor box. Considering flow rate, injection volume, and the total of the capillary volumes, Chromeleon converts the factor into a time after which the injection valve is switched to “Load”.

This option minimizes the gradient delay volume of an HPLC system, and thus reduces flush and equilibration times.

Tip:

A valve switch might not be carried out if the switch command interferes with other tasks of the sampler after an injection such as a wash step. A typical error message would be "Sampler is busy".

FlushVolume

Sets the flush volume to be used for the first Full-Loop, Partial-Loop, or microliter pick-up injection.

 Tip:  

For the WPS-3000FC, the flush volume must be equal to or larger than the needle volume + the bridge volume.

FlushVolume2

Sets the flush volume that shall be used for additional injections from the same vial for Full-Loop and Partial-Loop injections when RinseBetweenInjections=No.

GreenTray

Specifies which sample containers are installed in the green segment. Also, see BlueTray.

IncrementPositionCalculator

Specify the number of increments by which the sample position is to be extended (see PositionCalculator). For more information, refer to   Sample Position Commands (WPS-3000/ACC-3000).

InitiateChangeNeedle

Moves the needle arm to the left and the sample needle upwards to enable replacement of the needle.

InitiateChangeSyringe

Initiates the procedure for exchanging the syringe. (Also, see TerminateChangeSyringe.)

InitiateChangeVial

Rotates the sample containers specified by the Tray parameter to the front, allowing you to change the vials or plates easily. Select BlueTray, GreenTray, or RedTray to move the corresponding plate or tray to the front). Or else, select BlueVials, GreenVials, or RedVials to move the corresponding segment with the transport vials to the front.

Inject

Injects the sample. If you create a user-defined WPS-3000 program, e.g., in the  Program Wizard, use the Inject command to start the program. In this case, injection is performed after the UdpInjectMarker command of the user-defined program.

InjectionValvePosition

Reports the current position of the injection valve: Inject, Load, SwitchToInject, SwitchToLoad, or Unknown.

InjectMode

Injection mode (UserProg, Partial,  FullLoop,  µlPickUp,  Normal) (for more information, refer to the operating instructions for the instrument).

InjectValveToInject

Switches the injection valve into the Inject position.

InjectValveToLoad

Switches the injection valve into the Load position.

InjectWaitTime

Reports the time that has passed between the issuing of the injection command by Chromeleon and the response from the autosampler (read-only).

InjectWash

Specifies whether the wash procedure is part of the standard inject procedure (not for InjectMode = UserProg). Select an option:

• NoWash: no wash cycle is performed (recommended for typical applications),

• BeforeInj: performs a wash step before drawing a sample – and thus before sample injection (needle contains no sample liquid),

• AfterInj (pulled loop samplers with fractionation option only): performs a wash step after sample injection

• AfterDraw (split loop samplers only): after drawing a sample (and before the needle is moved to the injection port) the needle is washed externally

• Both: combines options BeforeInj and AfterInj (pulled loop samplers with fractionation option) or BeforeInj and AfterDraw respectively (split loop samplers)

For user-defined injection procedures, you have to set wash steps explicitly.

InstalledOn

Indicates the timebase(s) in which the autosampler is installed.

LastTransportVial

Sets the position for the last transport vial. Transport vials are required only for microliter pick-up injections. (Also, see FirstTransportVial.)

Leak

Reports whether the leak sensor detected a leak NoLeak or Leak.

LeakAlarmOff

Turns off the beep for the current alarm. A new beep sounds when the leak sensor detects another leak.

LeakSensorMode

The following options are available:
Enabled - Enables leak detection. If the sensor is activated, a message appears in the Chromeleon Audit Trail and an acoustic beep sounds.
Silent - Enables leak detection. If the sensor is activated, a message appears in the Chromeleon Audit Trail, but no beep sounds.
Disabled - Disables leak detection.

LoopDecompressionDelay

Specifies how long the needle remains in the needle seat when the injection valve was switched from Inject to Load before it moves to the sample vial.
Default: 2 ms/µl sample loop volume.

LoopOverfill

(Used only for full-loop injections)
The volume of the sample loop and the LoopOverfill factor determine the volume that is drawn in and/or transported through the sample loop before the sample is injected. Example:

Loop volume: 5 µl, LoopOverfill: 2 =
Volume to be drawn: 10 µl + flush volume

LoopVolume

Reports the volume of the installed sample loop (read-only).

LoopWashFactor

Factor for the loop wash volume. The contents of the buffer loop and the LoopWashFactor determine the loop wash volume. The default factor is 2 for micro and analytical HPLC applications, and 1.1 for semipreparative HPLC applications.

LowDispersionFactor

Determines the volume for Low Dispersion Injections. The volume is calculated by multiplying the original sample volume with the factor.

LowDispersionFlow

Sets the flow rate with which the sample is flushed out of the sample loop in Low Dispersion Mode.

LowDispersionMode

Enables low dispersion mode.

MaxInjectVolume

Sets the maximum inject volume of the application. This parameter is required for calculation of the Bypass Time (see also Flush Out Factor).

MaxPressure

Reports the maximum pressure that the current autosampler variant supports (read-only).

ModelVariant

Reports the autosampler variant (read-only).

MotorControllerVersion

Indicates the version of the motor controller (read-only).

MoveNeedleDown

Moves the needle to the position specified by NeedleHeight.

MoveNeedleHome

Moves the needle to the topmost position (Home position).

MoveTo

Moves the needle over the position specified with the Position property.

NeedleHeight

Determines to which position the needle moves with the MoveNeedleDown command.

NeedleVolume

Reports the volume of the installed needle (read-only).

Operable

Reports whether the autosampler is ready to operate.

Position

Sets the sample position on the carousel. For more information, refer to   Defining Sample Positions.

PositionCalculator

Calculates the sample position (for example, for the prep vial) from the current position. Use the IncrementPositionCalculator property to specify the number of positions to be incremented. For more information, refer to   Sample Position Commands (WPS-3000/ACC-3000).

PrepareNextSample

Starts the inject procedure for the next sample (if another sample is available). However, the inject valve will be switched at the next Inject command.

PrepareThisSample

Starts the inject procedure for the current sample. However, the injection valve will be switched at the next Inject command.

PrepVial

Sets the position for the sample container used for sample preparation. This position is used for UdpDraw and UdpDispense operations if PrepVial has been selected in the From and/or To fields of the UdpDraw and/or UdpDispense commands.

PrimeSyringe

Primes the syringe by filling and emptying it. Use PrimeSyringeNumber to determine how often the syringe is primed.

PrimeSyringeNumber

Specifies how often the syringe is filled and emptied during the PrimeSyringe operation.

PumpDevice

This property is available only if you have specified on the Segments/ Pump Link tab in the Chromeleon Server Configuration in the autosampler's properties that the injection command be synchronized with the strokes of an UltiMate 3000 series pump. Usually, the setting from the Segments / Pump Link tab page is displayed by default, e.g., MicroPump or Loading Pump.
If you want to change the synchronization assignment for a specific application, enter the pump in the input field. This will not change the standard setting on the Segments/ Pump Link tab page.

PunctureOffset

Moves the carousel by the specified value while the needle is in the septum, thus enlarging the hole in the septum and equalizing the pressure in the vial. Select this parameter when using large injection volumes and unslotted septa.

PunctureSpeed

Sets the speed of the puncturer.

PuncturerDownNeedleDown

Moves the puncturer and needle down.

PuncturerDownNeedleUp

Moves the puncturer down and the needle up.

PuncturerUpNeedleDown

Moves the puncturer up and the needle down.

PuncturerUpNeedleUp

Moves the puncturer and needle up.

PurgePumpOn

Moves needle to the wash port and draws mobile phase or air from the pump to reduce the pump priming time.

PurgePumpOff

Stops the purge pump.

ReagentLiquidHeight

Defines how deep the needle descends into the container for sampling. For details, refer to  Autosampler Control: Defining the Needle Height.

ReagentA/B/C/D/E/F/G/HVial

Indicates which vial (A, B, C, D, E, F, G, or H) is used in Draw/Dispense commands, if From/To is set to ReagentA/B/C/D/E/F/G/HVial. See also: UdpDraw/UdpDispense.

RedTray

Specifies which sample containers are installed in the red segment. Also, see BlueTray.

Relay4Enabled

Sets whether relay 4 is controlled as "normal" relay by Chromeleon, or used for the inject response signal.

Xsec_InjectOut: Depending on the specified duration, relay 4 will be active for this time upon each inject. This function can be used, for example, to automatically send a start signal to a mass spectrometer upon injection.

Select Yes for user-defined control via a Chromeleon program, i.e., if you want to control the relay via the Relay_4.On and "Relay_4.Off commands instead.

ReleaseExclusiveAccess

Releases the exclusive access of the current timebase to the autosampler.

Reset

Sets the autosampler to the initial conditions when the instrument was turned on.

RinseBetweenReinjections

Sets whether a wash cycle is performed after each injection. If set to Yes, a wash cycle is performed  after each injection. If set to No, the wash cycle is skipped when the next injection is performed from the same vial. (Read-only for the WPS-3000FC.)

 Notes: 

This command allows you to inject several times from the same vial without losing sample between the injections by rinsing.

A wash cycle is always performed after the last sample in a sequence.

SampleHeight

Defines how deep the needle descends into the container for sampling (standard injections). For details, refer to  Autosampler Control: Defining the Needle Height.

SampleHeightOffset_XX

Defines the bottom height of the sample container (= distance between the outer and inner bottom of the sample container). Also, see SampleHeight.

SeatCapillaryVolume

Reports the seat capillary volume in [µl].

SetFactoryDefaults

Resets the autosampler settings to the Dionex factory default settings.

SelfTest

Performs a self-test.

 Note:

You cannot start a batch when the sampler failed the self-test.

Standby

Sets the autosampler into the Standby mode or cancels this mode (NoStandby). Available values: Standby, NoStandby, WakingUp, EnteringStandby. (WakingUp and EnteringStandby are temporary states that the device assumes automatically, it is therefore neither required nor reasonable to select these values from the commands list.)

 Note:

You cannot start a batch when the sampler is in standby mode.

State

Reports whether the autosampler has injected: On (= injection has been performed) or Off (= injection has not yet been performed). (Read-only)

Status

Indicates which operation the autosampler is currently performing (read-only).

StopInject

Stops the running injection.

SyncWithPump

This property is available only if you have specified on the Segments tab in the autosampler's properties that the injection command be synchronized with the strokes of an UltiMate 3000 series pump. In this case, the setting is On. If you want to disable synchronization for a specific application, set the property to Off. (Also, see PumpDevice.)

 Note: 

SyncWithPump is automatically set to On when you change the PumpDevice setting.

Synchronization only takes place, however, if it is explicitly requested in the PGM file. Ensure the file contains a line SyncWithPump = On. Manually setting this property prior to starting the batch is not sufficient.

Syringe

Reports the volume of the installed syringe (read-only).

SyringeValvePosition

Reports the current position of the syringe valve: Needle, Wash, Waste, or Unknown.

SyringeWashFactor

Factor for the syringe wash volume. The syringe volume and the SyringeWashFactor determine the syringe wash volume. The default factor is 1.

TerminateChangeSyringe

Terminates the procedure for exchanging the syringe. (Also, see InitiateChangeSyringe.)

TransLiquidHeight

Defines how deep the needle descends into the container for sampling. For details, refer to  Autosampler Control: Defining the Needle Height.

TransportVialCapacity

Sets how often transport liquid can be drawn from one transport vial.

 Tip: 

Keep in mind that two draw operations are required for microliter pick-up.

TransportVialUsecount

Reports how often transport liquid was drawn from the transport vials.

 Tip: 

Reset the counter to zero after you have refilled the transport vials. If the maximum permissible number of draw operations, as defined with TransportVialCapacity, has been reached, a message ("out of transport liquid") is logged in the Chromeleon Audit Trail.

TransVialPunctureDepth

Sets how deep the puncturer descends into the transport vial, measured from the top of the transport vial.

TrayIllumination

Sets wether tray illumination is On or Off. When using light-sensitive samples, tray illumination should be set to Off.

Volume

Sets the injection volume for standard injections.

Wash

Performs a wash operation. The liquid volume used during the wash procedure can be set in the WashVolume command.

WashBufferLoop

Washes the buffer loop using the specified volume.

WashSampleLoop

Washes the sample loop.

WashSpeed

Sets the syringe speed for the wash operation.

WashVolume

Sets the volume that is drawn for the wash cycle (also see command Wash).

Notes:

• The ACC-3000 automatically performs a wash cycle after each injection.

• When using a WPS-3000SL/RS, WashVolume sets the volume for the wash procedure specified in the InjectWash command.

WasteSpeed

Sets the syringe speed for expelling liquid to the waste.

Command/Property

Description

ReadyTempDelta

Enter the allowable deviation of the current temperature from the target temperature. When the ReadyTempDelta is exceeded,  the autosampler is not ready to operate (TemperatureReady = NotReady).

TempCtrl

Enables temperature control of the sample rack (On). To deactivate temperature control, set to Off.

Temperature

Sets the target temperature for the carousel and thus, for the sample. Click the "+" character beside the name to display the items underneath: Value (reports the actual temperature), Nominal (sets the target temperature), UpperLimit (sets the upper temperature limit), and LowerLimit (sets the lower temperature limit). The system aborts the sample batch and/or starts error handling if the nominal temperature is outside the specified limits.

TemperatureReady

Reports whether the autosampler is ready to operate. When the current temperature deviates from the target temperature by more than the ReadyTempDelta, Chromeleon reports NotReady (read-only).

Command/Property

Description

CapillaryDriveMovements

Counts how many times the needle capillary has moved.

CoolingWorkLoad

Reports the total cooling work load [K*min] since the module has been shipped (read-only).

DrainPumpHours

Counts the operating time [h] of the peristaltic pump (read-only).

DrainPumpTubeChanged

After you have replaced the peristaltic pump tube, perform this command to update the related service information. The DrainPumpTubeChangeDate counter is automatically set to the current date; the DrainPumpTubeHours are reset to "0".

DrainPumpTubeChange
Date

Reports the date when the peristaltic pump tube was changed last (read-only).

DrainPumpTubeHours

Counts the operating hours of the peristaltic pump with the currently installed tube.

*.Value

Reports the current operating hours of the peristaltic pump (read-only) with the currently installed tube.

*.Warning

A warning appears when the specified value is reached.

*.Limit

An error message appears when the specified value is reached.

HeatingWorkLoad

Reports the total heating work load [K*min] since the module has been shipped (read-only).

HorizontalDrive
Movements

Counts how many times the horizontal drive moves into a new position (read-only).

InjectValveSwitches

Counts how many times the injection valve switches (read-only).

NeedleChangeDate

Reports the date when the needle was replaced last (read-only).

NeedleChanged

After you have replaced the needle, perform this command to update the related service information. The NeedleChangeDate is automatically set to the current date; the NeedleMovements and NeedleObstructions counters are reset to "0".

NeedleDriveMovements

Counts how many times the needle drive moves into a new position (read-only).

NeedleMovements

Counts how many times the needle moves downwards into the needle seat.

*.Value

Reports the current number of movements (read-only).

*.Warning

A warning appears when the specified value is reached.

*.Limit

An error message appears when the specified value is reached.

NeedleObstructions

Counts how many times the needle descends on an obstacle.

NeedleSeatChanged

After you have replaced the needle seat, perform this command to update the related service information. The NeedleSeatChangeDate is automatically set to the current date; the NeedleSeatCounter is reset to "0".

NeedleSeatChangeDate

Reports when the needle seat was replaced last (read-only).

NeedleSeatCounter

Counts how many times the needle moves into the needle seat.

*.Value

Reports the current number of needle movements Into the needle seat (read-only).

*.Warning

A warning appears when the specified value is reached.

*.Limit

An error message appears when the specified value is reached.

OperatingHours

Counts the operating hours since initial operation (excluding the operating hours in standby mode; read-only).

PowerOnTime

Counts the time since last power-up (or last wake-up from standby) (read-only).

PurgePumpHours

Counts the operating hours of the purge pump (read-only).

Qualification

Provides information about system qualification.

*.LastDate

Reports the date when autosampler qualification was performed last (read-only).

*.LastOperator

Reports the name of the person who performed the autosampler qualification (read-only).

*.Interval

Reports the interval (in days) when the next system qualification is due.

*.WarningPeriod

Specifies the period during which a warning appears before the next service is due.

*.GracePeriod

Indicates the period during which the device may be operated after the due date for re-qualification.

QualificationDone

Perform this command after qualification to update the related system data. The Qualification - LastDate is automatically set to the current date, the Qualification - LastOperator is set to the current user.

RotorSealChanged

After you have replaced the rotor seal, perform this command to update the related service information. The RotorSealChangeDate counter is automatically set to the current date; the RotorSealSwitches are reset to "0".

RotorSealChangeDate

Reports when the rotor seal was replaced last (read-only).

RotorSealSwitches

Counts how many times the injection valve has switched since the rotor seal was replaced last.

*.Value

Reports the current number of injection valve switchings (read-only).

*.Warning

A warning appears when the specified value is reached.

*.Limit

An error message appears when the specified value is reached.

Service

Provides service information.

*.LastDate

Reports the date of the last service (read-only).

*.Interval

Specify the interval (in days) after which a service is due.

*.WarningPeriod

Specifies the period during which a warning appears before the next service is due.

ServiceDone

After the device has been serviced, perform this command to update the related information. The Service - LastDate property is automatically set to the current date.

StandbyHours

Counts the total operating hours in standby mode since the module was operated for the first time (read-only).

SyringeChanged

After you have replaced the syringe, perform this command to update the related service information. The SyringeChangeDate counter is automatically set to the current date; the SyringeMovements counter is reset to "0".

SyringeChangeDate

Reports when the syringe was replaced last (read-only).

SyringeDriveMovements

Counts how many times the syringe drive moves into a new position (read-only).

SyringeMovements

Counts how many times the syringe moves into a new position.

*.Value

Reports the current value of syringe movements (read-only).

*.Warning

A warning appears when the specified value is reached.

*.Limit

An error message appears when the specified value is reached.

SyringeValveCounter

Counts how many times the syringe valve switches (read-only).

TotalInjections

Counts the number of injections (read-only).

TrayDriveMovements

Counts how many times the tray moves to a new position (read-only).