Examples for User-Defined Programs for the WPS-3000 Autosamplers

Autosampler Control
Examples for User-Defined Programs for the WPS-3000 Autosamplers

The user-defined program is loaded and executed at the autosampler's start time (Sampler.Inject command) even if the program steps appear prior to that command in the program file. Use the PGM Wizard to create a user-defined program. You can either create a new user-defined program or use an existing program as a template for the new one. Make sure that the user-defined program includes an UdpInjectMarker command at the appropriate position so that the system recognizes that an injection has been made:

The following programs are typical application examples for the WPS-3000PL (RS) Autosampler:

User-defined standard program


 ;User defined program (UDP) template for WPS-3000 sampler
 ;****** PLEASE DO NOT EDIT ******
0.000 InjectMode = UserProg
      ReagentAVial = R1
      ReagentBVial = R2
      ReagentCVial = R3
      ReagentDVial = R4
      ;PrepVial = Position+5
      ;Relative vial positions should be defined using the "PositionCalculator" property,
      as shown in the following three lines
      PositionCalculator = Sampler.Position
      IncrementPositionCalculator By=5
      PrepVial = PositionCalculator
      
      UdpDraw From=SampleVial, Volume=5, SyringeSpeed=50, SampleHeight=2
      UdpDispense To=PrepVial, Volume=5, SyringeSpeed=100, SampleHeight=2
      UdpDraw From=ReagentAVial, Volume=10, SyringeSpeed=50, SampleHeight=2
      UdpDispense To=PrepVial, Volume=10, SyringeSpeed=2000,  SampleHeight=2
      UdpMixWait Duration=10
      UdpDraw From=PrepVial, Volume=5, SyringeSpeed=50, SampleHeight=2
      UdpInjectValve Position=Inject
      UdpInjectMarker
      Inject       ;starts the above program and waits for the inject response.     
      Acquisition On
      ...
4.000 Acquisition Off
      End

Drawing a sample volume of 1 µl


 ;User defined program (UDP) template for WPS-3000 sampler
 ;****** PLEASE DO NOT EDIT ******
0.000 InjectMode = UserProg
      UdpInjectValve Position=Load
      UdpSyringeValve Position=Needle
      UdpDraw From=SampleVial, Volume=1.0, SyringeSpeed=50, SampleHeight=2
      UdpMixWait Duration=5
      UdpDraw From=SampleVial, Volume=0.0, SyringeSpeed=50, SampleHeight=2
      UdpDraw From=ReagentAVial, Volume=25, SyringeSpeed=50, SampleHeight=2
      UdpMixWait Duration=5
      UdpInjectValve Position=Inject
      UdpInjectMarker      ;generates an Inject Response during the program run
      UdpMixWait Duration=5
      UdpDraw From=ReagentAVial, Volume=0.0, SyringeSpeed=50, SampleHeight=2
      UdpSyringeValve Position=Waste
      UdpMoveSyringeHome SyringeSpeed=2000
      UdpSyringeValve Position=Needle
      UdpMixNeedleWash Volume=50
      Inject      ;starts the above program and waits for the inject response.
      Acquisition On
      ...
4.000 Acquisition Off
      End

Sample preparation with 2 reagents


;User defined program (UDP) template for WPS-3000 sampler
 ;****** PLEASE DO NOT EDIT ******
0.000 InjectMode = UserProg
      UdpDraw From=ReagentAVial, Volume=20.0, SyringeSpeed=50, SampleHeight=2
      UdpMixWait Duration=5
      UdpDraw From=ReagentAVial, Volume=0.0, SyringeSpeed=50, SampleHeight=2
      UdpMixWait Duration=5
      UdpDispense To=SampleVial, Volume=20.0, SyringeSpeed=2000, SampleHeight=2
      UdpMixWait Duration=10
      UdpDraw From=ReagentBVial, Volume=10.0, SyringeSpeed=50, SampleHeight=2
      UdpMixWait Duration=5
      UdpDraw From=ReagentBVial, Volume=0.0, SyringeSpeed=50, SampleHeight=2
      UdpMixWait Duration=5
      UdpDispense To=SampleVial, Volume=10.0, SyringeSpeed=2000, SampleHeight=2
      UdpMixWait Duration=10
      UdpInjectValve Position=Inject
      UdpSyringeValve Position=Needle
      UdpDraw From=SampleVial, Volume=3.0, SyringeSpeed=50, SampleHeight=2
      UdpMixWait Duration=5
      UdpInjectValve Position=Load
      UdpDraw From=SampleVial, Volume=5.0, SyringeSpeed=50, SampleHeight=2
      UdpMixWait Duration=5
      UdpInjectValve Position=Inject
      UdpInjectMarker      ;generates am inject response during the program run
      UdpMixWait Duration=5
      UdpDraw From=SampleVial, Volume=0.0, SyringeSpeed=50, SampleHeight=2
      UdpSyringeValve Position=Waste
      UdpMoveSyringeHome SyringeSpeed=2000
      UdpSyringeValve Position=Needle
      UdpMixNeedleWash Volume=50
      Inject      ;starts the above program and waits for the inject response.
      Acquisition On
      ...
4.000 Acquisition Off
      End

Gel extraction


 ;User defined program (UDP) template for WPS-3000 sampler
 ;****** PLEASE DO NOT EDIT ******
0.000 InjectMode = UserProg
      UdpDraw From=SampleVial, Volume=5.0, SyringeSpeed=50, SampleHeight=2
      UdpDispense To=PrepVial, Volume=5.0, SyringeSpeed=2000, SampleHeight=2
      UdpDraw From=ReagentAVial, Volume=5.0, SyringeSpeed=50, SampleHeight=2
      UdpDispense To=SampleVial, Volume=5.0, SyringeSpeed=2000, SampleHeight=2
      UdpMixWait Duration=10
      UdpDraw From=SampleVial, Volume=5.0, SyringeSpeed=50, SampleHeight=2
      UdpDispense To=PrepVial, Volume=5.0, SyringeSpeed=2000, SampleHeight=2
      UdpDraw From=ReagentBVial, Volume=5.0, SyringeSpeed=50, SampleHeight=2
      UdpDispense To=PrepVial, Volume=5.0, SyringeSpeed=2000, SampleHeight=2
      UdpMixWait Duration=10
      UdpDraw From=SampleVial, Volume=5.0, SyringeSpeed=50, SampleHeight=2
      UdpDispense To=PrepVial, Volume=5.0, SyringeSpeed=2000, SampleHeight=2
      UdpMixWait Duration=5
      UdpInjectValve Position=Inject
      UdpSyringeValve Position=Needle
      UdpDraw From=SampleVial, Volume=1.0, SyringeSpeed=50, SampleHeight=2
      UdpMixWait Duration=5
      UdpInjectValve Position=Load
      UdpDraw From=PrepVial, Volume=5.0, SyringeSpeed=50, SampleHeight=2
      UdpMixWait Duration=5
      UdpInjectValve Position=Inject
      UdpInjectMarker      ;generates an inject response during the program run
      UdpMixWait Duration=5
      UdpDraw From=SampleVial, Volume=0.0, SyringeSpeed=50, SampleHeight=2
      UdpSyringeValve Position=Waste
      UdpMoveSyringeHome SyringeSpeed=2000
      UdpSyringeValve Position=Needle
      UdpMixNeedleWash Volume=50
      Inject      ;starts the above program and waits for the inject response
      Acquisition On
      ...
4.000 Acquisition Off
      End

Microliter pick-up


Sampler.InjectMode = UserProg
        Sampler.ReagentAVial=   R1
        Sampler.UdpDispense     To=Drain, Volume=0.000, SyringeSpeed=GlobalSpeed, SampleHeight=GlobalHeight
        Sampler.UdpSyringeValve Position=Needle
        Sampler.UdpInjectValve  Position=Inject
        Sampler.UdpDraw         From=ReagentAVial, Volume=5.000, SyringeSpeed=GlobalSpeed, SampleHeight=GlobalHeight    ;A
        Sampler.UdpMixWait              Duration=5
        Sampler.UdpInjectValve  Position=Load
        Sampler.UdpDraw         From=SampleVial, Volume=10.000, SyringeSpeed=GlobalSpeed, SampleHeight=GlobalHeight     ;B
        Sampler.UdpMixWait              Duration=5
        Sampler.UdpDraw         From=ReagentAVial, Volume=5.000, SyringeSpeed=GlobalSpeed, SampleHeight=GlobalHeight    ;C
        Sampler.UdpMixWait              Duration=5
        Sampler.UdpDispense     To=Drain, Volume=0.000, SyringeSpeed=GlobalSpeed, SampleHeight=GlobalHeight
        Sampler.UdpInjectValve  Position=Inject
        Sampler.UdpInjectMarker

Notes:

A+B+C should always be equal or preferentially larger than (sample loop + needle volume)
A is the plug of reagent liquid that is at the back side of the sample loop and the sample plug B. The higher the sample volume B, the less volume of A remains in the sample loop
B is the sample volume. This volume can be replaced by the property Sampler.Volume
C Is the needle volume (it is preferentially a bit larger than that to overcome standard deviations on the loop dimensions and the extra port-to-port volume)
The given example is not applicable to the WPS-3000FC! For the FC there is an extra bridge volume to be taken in account for volume C!
The example should work for autosamplers with both 8-port valve and 6-port valve

In-needle mixing


 ;User defined program (UDP) template for WPS-3000 sampler
 ;****** PLEASE DO NOT EDIT ******
0.000 InjectMode = UserProg
      ReagentAVial = BA1; Reagent 1: for example buffer
      ReagentBVial = BA2; Reagent 2
      ; Reagent 1 and sample solution are drawn into the needle
      UdpDraw From=ReagentAVial, Volume=5.000, SyringeSpeed=1.000, SampleHeight=GlobalHeight
      UdpDraw From=SampleVial, Volume=1.000, SyringeSpeed=1.000, SampleHeight=GlobalHeight
      ; Reagent 1 and sample solution are mixed in the needle  by drawing and dispensing air
      (here: mixing volume 6 µL, repetition: three times)

UdpDraw        From=Air, Volume=6.000, SyringeSpeed=1.000, SampleHeight=GlobalHeight
UdpMoveSyringe Unload=6.000, SyringeSpeed=33.000
UdpMoveSyringe Load=6.000, SyringeSpeed=33.000
UdpMoveSyringe Unload=6.000, SyringeSpeed=33.000
UdpMoveSyringe Load=6.000, SyringeSpeed=33.000
UdpMoveSyringe Unload=6.000, SyringeSpeed=33.000
; Waiting time for reaction (here: 15 s) followed by needle wash
UdpMixWait       Duration=15
UdpMixNeedleWash Volume=100.000  
; Reagent 2 is drawn followed by in-needle-mix with previous drawn solutions 
(here: mixing volume 7 µL,  mixing: six times)
UdpDraw        From=ReagentBVial, Volume=1.000, SyringeSpeed=1.000, SampleHeight=GlobalHeight
UdpDraw        From=Air, Volume=7.000, SyringeSpeed=1.000, SampleHeight=GlobalHeight
UdpMoveSyringe Unload=7.000, SyringeSpeed=33.000 
UdpMoveSyringe Load=7.000, SyringeSpeed=33.000 
UdpMoveSyringe Unload=7.000, SyringeSpeed=33.000 
UdpMoveSyringe Load=7.000, SyringeSpeed=33.000 
UdpMoveSyringe Unload=7.000, SyringeSpeed=33.000
UdpMoveSyringe Unload=7.000, SyringeSpeed=33.000 
UdpMoveSyringe Load=7.000, SyringeSpeed=33.000
UdpMoveSyringe Unload=7.000, SyringeSpeed=33.000 
UdpMoveSyringe Load=7.000, SyringeSpeed=33.000
UdpMoveSyringe Unload=7.000, SyringeSpeed=33.000 
; Waiting time for reaction (here: 15 s) followed by needle wash
UdpMixWait       Duration=15
UdpMixNeedleWash Volume=100.000
; injection of total in-needle mixture 
(here: 5 µL Reagent 1 + 1 µL Sample + 1 µL Reagent 2 = 7 µL total)
UdpInjectMarker
UdpInjectValve     Position=Inject
UdpSyringeValve    Position=Waste
UdpMoveSyringeHome SyringeSpeed=GlobalSpeed

For an overview of the individual commands that are available for the autosampler, refer to Commands for Controlling Dionex Devices UltiMate 3000 Series - Autosampler.

For information about the sample preparation commands supported by the autosampler, refer to Commands for Controlling Dionex Devices Dionex WPS-3000 Autosamplers: Commands for User-Defined Programs.