Instrument Type:
Related Commands:
Description:
The Inject command defines the beginning of a chromatogram, that is, it determines the time at which the sample enters the high-pressure system. Thus, the time of the first inject command is 0 by definition. The advantage is that the Retention Time, as appearing in chromatograms and reports, coincides with program execution times. All commands before inject, are assigned negative execution times.
Between the Inject command and the actual injection process (Inject response by the autosampler or the hand-operated valve), a system-dependent interval is required for reaching the rack and to draw the sample. During this time, the pump remains in Hold state; that is, a gradient that might be running is stopped. The Inject Wait state is canceled only by an Inject Response signal. The response signal is delivered either automatically (via an interface) or via a remote input signal. Only from this point, time keeping is started.
Function:
The function depends on the individual installation. For manual injection, Chromeleon simply holds the analysis time and waits for inject response. For a controlled autosampler, the inject command (at least) is relayed to this. For autosamplers with variable injection volumes and random sample access, such as the ASI-100 autosampler, the inject volume and vial position are relayed as well. Such autosamplers are ideal for automated multipoint calibration.
For interactive operation (online) of the inject command, the Sample Position and Inject Volume parameters must be entered as well. During automated batch operation, these values are read from the sequence. Thus, parameters do not have to be set individually in the Program.
Parameters:
(When a hand-operated valve is used for injection, this information can be used for documentation purposes.)
Position
Vial Position in the autosampler
Inject Volume
Injection Volume in µl
Blank
Specifies whether Blank Run Samples are actually injected:
Skip: no injection
Inject: Injection is performed.
When you use the Famos autosampler, make sure to allow a short time before blank run samples are injected. (For more information on the autosampler, refer to Hardware Installation 
FAMOS Capillary/Nano HPLC Autosampler in the Administrator Help section.)
-0.100 ; 6 seconds to process program
0.000 Inject
Tips:
Data acquisition (AcqOn/Off) should be started after the Inject signal, at least 30 seconds before the first peak. Also, there should be Isocratic conditions during the injection process.
A program should only contain one inject command.
For the ICS ion chromatography system, injection is performed with the Pump_InjectValve.InjectPosition command (if no AS50 is installed). With the Pump_InjectValve.InjectPosition command, an injection is always performed, also for a Blank Run Sample. This is contrary to the autosampler Inject command.