Dionex Autosamplers
Dionex WPS-3000 Autosamplers: Commands for User-Defined Programs

The Dionex WPS-3000 provide various sample preparation commands that are used to further define the individual sample preparation steps. The sample preparation commands are accessible from the Advanced level only. Use the  Program Wizard to create a user-defined sample preparation program. This program is started by the Inject command.

Chromeleon supports the following sample preparation commands (please note that the display Filter level determines which commands and properties are displayed):

 

Command/Property

Description

UdpAirPump

Sets whether outside air shall be used to dry the needle.
Select On or Off.

UdpDispense

Dispenses a specified volume of liquid. Make the following settings:

To: Enter the position to which the specified volume is delivered.

Volume: Enter the volume to be dispensed (0.0 to 25.0 µl).

SyringeSpeed: Specify the speed with which the volume is expelled (10 to 8333 nl/s; option: GlobalSpeed here = DispSpeed).

SampleHeight: Determine how deep the needle descends into the sample container (0 to 40 mm; option: GlobalHeight = SampleHeight).

UdpDraw

Draws a specified volume of liquid. Make the following settings:

From: Enter the position from which the specified volume is drawn.

Volume: Enter the volume to be drawn (0.0 to 25.0 µl).

SyringeSpeed: Specify the speed with which the volume is drawn (10 to 8333 nl/s; option: GlobalSpeed here = DrawSpeed).

SampleHeight: Determine how deep the needle descends into the sample container (0 to 40 mm; option: GlobalHeight here = SampleHeight).

UdpInjectMarker

Generates an inject marker pulse. This is required in user-specific programs, i.e., when InjectMode = UserProg. The injection can be performed only after this pulse.

UdpInjectValve

Switches the injection valve to the specified position:
Inject or Load.

UdpMixNeedleWash

Sets the volume that is used for washing the needle during sample preparation.

UdpMixWait

Sets the time that the system waits between the single sample
preparation steps.

UdpMoveSyringe

Moves the syringe. Enter the volume to be drawn in the Load field; or else, enter the volume to be dispensed in the Unload field. Only set one of these parameters. Enter the speed for the syringe movement in the SyringeSpeed field.

 Tip:

Make sure that both parameters appear in the same line of the PGM File. If they appear in different lines, the PGM File does not pass the Ready Check.

UdpMoveSyringeHome

Moves the syringe it to its Home position and empties it.

UdpOutput

Sets the specified output into the defined state. Select the output
(Out1 ... Out4) and the state (On or Off) from the associated
drop-down lists.

UdpSyringeValve

Switches the syringe valve to the specified position:
Needle, Wash, or Waste.

UdpWaitInput

Waits until the specified input is in the selected state. Select the input
(Inp1 ... Inp4) and the state (Low or High) from the associated drop-down lists.

UdpWaitStrokeSync

Waits with the continuation of a program for InjectMode = UserProg until the pump has sent the synchronization signal. (Also, see PumpDevice, SyncWithPump.)

 

For a basic description of Dionex autosamplers, including the standard commands, refer to  Dionex Autosamplers.

For information about the general commands for the autosampler, refer to  UltiMate 3000 Series - Autosampler.

For information about how to use the commands in practical operation, refer to Practical Tips for Device Control  Examples for User-Defined Programs for the WPS-3000 Autosamplers.