Agilent 1100/1200 HPLC System: Autosampler (G1329)

Commands and Tips for Third-Party Devices
Agilent 1100/1200 HPLC System: Autosampler (G1329)

In addition to (or different from) the standard autosampler commands (see Commands for Controlling Dionex Devices Dionex Autosamplers) and the general commands for the 1100 and 1200 HPLC systems (see Agilent 1100/1200 HPLC System: General), the following commands and properties are available (please note that the display Filter level determines which commands and properties are displayed):

Property	Min	Max	Default	Description
DispSpeed	10 µl/min or 90 µl/min	1000 µl/min	200 µl/min	Speed for dispensing the sample
DrawSpeed	10 µl/min or 90 µl/min	1000 µl/min	200 µl/min	Speed for drawing the sample
Position	0	(Tray dependent)	n/a	Sample vial number
SampleHeight	-2.5 mm	35 mm	0.0 mm	Needle draw/eject position offset
Syringe	100 µl	900 µl	as configured	Syringe volume [µl]
Volume	0.0 µl	8000.0 µl	5.0 µl	Injection volume
WashVial	0	Greatest sample position (tray dependent)	0	Wash vial number 0: no wash

Command	Description
Dispense	Injects sample into the loop.
Draw	Draws sample from a vial into the syringe.
InitiateChangeGripper	Initiates the gripper change by moving the gripper to the front.
InitiateChangeNeedle	Initiates the needle change by removing the safety covering and moving the needle carrier to an easily accessible mounting position. In addition, the needle is raised to the uppermost position.
InitiateChangePiston	Initiates the piston change by releasing the spring on the proportioning unit.
Inject	Performs an injection (see note below).
Mix	Mixes the sample using the syringe.
Repeat/EndRepeat	Repeats the enclosed sample preparation steps.
TerminateChangeGripper	Terminates the gripper change by returning the gripper to the Home position.
TerminateChangeNeedle	Terminates the needle changes by returning the needle to the Home position.
TerminateChangePiston	Terminates the piston change by tensing the spring on the proportioning unit.
Valve	Switches the injection valve.
Wait	Waits a given time during the injection program.

Parameters of the commands

Command	Values	Description
Dispense, parameter: Vial	SampleVial (current sample vial) SampleVialPlus (current sample vial + n) Vial (sample vial No. n) Seat (needle seat) Air	Position of the vial for dispense
Dispense/ Mix, parameter: Speed	10 (or 90 µl) to 1000 µl/min Maximum Default (uses DispSpeed)	Speed for dispense or mix
Draw, parameter: Source	SampleVial (current sample vial) SampleVialPlus (current sample vial + n) Vial (sample vial No. n) Seat (needle seat) Air	Position of the vial for draw
Draw, parameter: Speed	10 (or 90) to 1000 µl/min Maximum Default (uses DrawSpeed)	Speed for draw
Draw/Dispense, parameter: Offset	-2.5 to 35.0 mm SampleHeight	Needle draw/eject position offset for draw or dispense
Draw/ Dispense/ Mix, parameter: Volume	0.0 to 100.0 µl (or 900.0 µl) Maximum InjectVolume	Volume for draw, dispense, or mix
Needle, parameter: Vial	SampleVial (current sample vial) SampleVialPlus (current sample vial + n) Vial (sample vial No. n)	Position to which the needle is moved

Tips:

The Inject command sends a start impulse to all modules. Then the analysis will start with the following steps:

The detector performs an Autozero if the program file contains the corresponding command at the time t=0.000 min.
An injection/sample preparation program is executed if the program contains the corresponding commands at the t=0.000 min.
The injection will take place and the run will start.

Three different injection programs are available:

Standard (injection volume from the respective position)	WashVial=None and no sample preparation commands
Standard (with needle wash)	WashVial=None and no sample preparation commands
User-defined injection program	If sample preparation commands are issued

Autosampler/Thermostat

Property	Min/Max	Default	Description
AutoActivateSetpoint	Off/On	On	If set to On, temperature control is automatically enabled right after power-up.
TempCtrl	Off/On	Off	Turns temperature control on or off. If set to On, temperature control is activated and the current temperature as shown under Temperature.Value is used as the temperature setpoint. To define a different setpoint, specify an appropriate value under Temperature.Nominal.
ThermoPower	Off/On	On	Turns the power supply for the thermostat on or off. If set to Off, it is not possible to start runs. This also applies if a fraction collector (G1364) is used.
Temperature.Nominal	4°C/40°C	20°C	Temperature set point for the air stream and the temperature control. If a nominal value is specified, the TempCtrl and ThermoPower properties are automatically set to On.
Temperature.Value	4°C/40°C	n/a	Current temperature of the air stream. This temperature value is used as the setpoint for the temperature control, if TempCtrl = On, but no Temperature.Nominal value is specified. Also refer to TempCtrl and Temperature.Nominal.

Also, see Agilent HPLC System 1100/1200: Wellplate Sampler (G1367).

Diagnostic Commands

Command	Description
GripperHome	Moves the gripper to home position.
GripperPick	Picks the vial at the current position.
GripperPut	Positions the vial at the current position.
GripperToPosition	Moves the gripper to the specified position.
NeedleIntoSeat NeedleIntoVial NeedleToPosition	(For the G1329 autosampler, the commands have no functionality.)
NeedleUp	Moves the needle to its topmost position.
PlungerDraw	Draws the plunger to load sample into the sample loop.
PlungerHome	Moves the plunger back to its home position.
VialToSeat	Positions the vial in the seat.
VialToTray	Positions the vial in the tray.
ValveToBypass	Switches the sample loop into the bypass position.
ValveToMainpass	Switches the sample loop into the inject position.

For more information about the diagnostic functions for the autosampler, refer to:

Agilent 1100 HPLC System Diagnostics: Injector Steps

Agilent 1100 HPLC System Diagnostics: Gripper Verification

The Administrator Help section provides installation instructions for the 1100 and 1200 HPLC systems; refer to Hardware Installation:

Agilent 1100 HPLC System: Installation

Agilent 1200 HPLC System: Installation