Commands and Tips for Third-Party Devices
Agilent 1100/1200 HPLC System: Wellplate Sampler (G1367)

In addition to the commands supported by the autosampler of the 1100/1200 HPLC System (see  Agilent 1100/1200 HPLC System: Autosampler (1329)), the wellplate sampler supports the same special commands (please note that the display Filter level determines which commands and properties are displayed). Please note: For some of the Agilent 1100/1200 autosampler commands, the ranges and parameters may be different for the wellplate sampler (refer to the table below).

 

Special Wellplate Sampler Parameters and Values

Command

Values

Description

Dispense,
parameter: Source

SampleVial (current sample vial)
Location (sample position No. n)
Seat (needle seat)

SampleVialPlus (current sample position + n)

Position of the vial for dispense

Dispense/ Mix,
parameter: SyringeSpeed

10-1000 µl/min
Maximum
Default (uses DispSpeed)

Speed for dispense or mix

DispSpeed

10-1000 µl/min

Speed for ejecting the sample

Draw,
parameter: Source

SampleVial (current sample vial)
Location (sample position No. n)
Seat (needle seat)
Air

SampleVialPlus (current sample position + n)

Position of the vial for draw

Draw,
parameter: SyringeSpeed

10-1000 µl/min
Maximum
Default (uses DrawSpeed)

Speed for draw

Draw/Dispense,
parameter: Offset

-10.0 to 60.0 mm
SampleHeight

Needle draw/eject position offset for draw or dispense

Draw/Dispense/ Mix,
parameter: Volume

0.00-100.00 µl
Maximum
InjectVolume

Volume for draw, dispense, or mix

DrawSpeed

10-1000 µl/min

Speed for drawing the sample

Needle,
parameter: Vial

SampleVial (current sample vial)
Location (sample position No. n)

Position to which the needle is moved

Park

 

Moves the transport arm to a park position. Use this command to prepare the sampler for transport.

SampleHeight

-10.0 to 60.0 mm

Needle draw/eject position offset

Volume

0.0-100.0 µl

Injection volume

WashVial

Sample position No.
no wash
flush (see WashTime)

Wash vial number. If the setting is no wash, a wash cycle is not performed.

 

 

Special Wellplate Sampler Commands

Property

Range/Values

Description

DelayVolumeReduction²

Off/On

Enables or disables automatic delay volume reduction.

EquilibrationTime³

0.0 s - 100.0 s

Time until equilibration is complete.

FlushFactor

0.1 - 10.0

or

0.1 - 20.0 (G1367 SL only)

Indicates how often the flush port is filled for flushing the needle (exterior).

PrimePump

1 - 1000

Primes needle for the time specified (in [s]).

WashRepeat

1 - 5

Number of washes when WashVial is set to a vial location.

WashTime

0.0 s - 100.0 s

Time to wash when WashVial is set to Flush.

 Tip: 

² Switches the injection valve back to BYPASS after the sample is eluted beyond the injection valve.

³ On connect of the wellplate sampler EquilibrationTime = 0.0 s is sent to the device. If necessary, enter another value explicitly.

 

Command

Options

Wash

In

Time

Repeat

Offset

(For user-defined inject programs)

In =
AsMethod,
Flush,
SampleVial or
osition of sample

Time =
0.0 to 100.0 s

Repeat =
1 to 5

Offset =
-10.0 to 60.0 mm

Function

Defines the location of the wash solution

See WashTime
required if
In = Flush

See WashRepeat
required if
In > 0 or SampleVial

Height of the needle above the bottom of the vial

 

For example, the following parameters are possible for the Wash command:

1. Wash In=Flush Time=3
(Washes for 3 seconds, using the eluent from the flush port.)

2. Wash  In=SampleVial Repeat=2
(Washes twice, using the solution from the current sample position.)

3. Wash  In=15 Repeat=5
(Washes five times, using the solution from sample position 15.)

4. Wash  In=AsMethod
(With this option, the parameters entered via the WashVial, WashTime, and WashRepeat commands are used.)

Also, see  Agilent 1100/1200 HPLC System: Autosampler (G1329).

 

Additional Commands for the G1367 SL Autosampler (Agilent Series 1200)

Property

Range/Values

Description

BottomSensing

On/Off

Determines the draw position of the needle.

Light

On/Off

Turns the sampler's tray lighting on or off.

ValveCleaning

 

Switches the valve after the sample flush-out time for the specified number of times and duration to automatically clean the system during gradient runs.

Note:

During each ValveCleaning step, the valve is switched twice: from mainpass to bypass and back to mainpass.

CleanTime_1:

Off...10000000.0 min

Set a time for CleanTime1 to switch the valve directly after the sample has been flushed out at a high volume percent water. Recommended for samples that are highly soluble in water.

See also comment under Note below.

CleanTime_2:

Off...10000000.0 min

Set a time for CleanTime2 to switch the valve at high % organic phase and to remove sample from the injection valve grooves.

Recommended for organic samples to avoid sample retardation on the column.

CleanTime_3:

Off...10000000.0 min

Set a time for CleanTime3 to switch the valve at low % organic phase and to remove organic solvent from injection valve grooves.

Recommended for organic samples.

CleanTime_4:

Off...10000000.0 min

Use CleanTime4 to repeat valve switch 1, 2 or 3 in the case of extremely sticky sample or solvent.

ValveMovements:

0...2

Select the number of successive valve switches.

 

The following recommendations for the Valve switching times (Time1 - Time3) during gradient run apply:

Whereby Time1 will be calculated as follows:

Time1= Sample flush-out factor * Total flush-out volume/Flow rate

Total flush-out volume = Injection volume + seat capillary volume + valve volume

Valve volume = 1.7 µl for the G1367A µ-WPS, 80 nl for the G1377A µWPS

Diagnostic Commands

 

Command

Description

GripperHome
GripperPick
GripperPut
GripperToPosition

(For the G1367 autosampler, the commands have no functionality.)

NeedleIntoSeat

Moves the needle into the needle seat.

NeedleIntoVial

Lowers the needle into the vial.

NeedleToPosition

Moves the needle to the specified position.

NeedleUp

Moves the needle to its topmost position.

PlungerDraw

Draws the plunger to load sample into the sample loop.

PlungerHome

Moves the plunger back to its home position.

VialToSeat
VialToTray

(For the G1367 autosampler, the commands have no functionality.)

ValveToBypass

Switches the sample loop into the bypass position.

ValveToMainpass

Switches the sample loop into the inject position.

 

For more information about the diagnostic functions for the autosampler, refer to  Agilent 1100 HPLC System Diagnostics: Injector Steps.

The Administrator Help section provides installation instructions for the 1100 and 1200 HPLC systems; refer to Hardware Installation:

 Agilent 1100 HPLC System: Installation

 Agilent 1200 HPLC System: Installation