Autosampler Control
Injecting a Sample

There are two methods for injecting a sample using a Chromeleon-controlled Autosampler:

• Perform the Inject command from the menu, toolbar, or control panel

• Perform the Inject command from a Program

In either case, the autosampler performs the injection and then reports to Chromeleon when injection is complete. When Chromeleon receives this message, the retention time clock is started.

To perform the injection from the Control menu or a control panel

• Select the Inject command on the Control menu or click the following icon on the Online Toolbar:  .
  In the Inject dialog box, enter the injection Volume and specify the position from which to perform the injection.

• If the control panel provides the corresponding controls, enter the Volume and Position directly in the corresponding fields, and then click Inject to start the injection.

If you want the time required by the autosampler to inject the sample to be visually indicated on the control panel, link a color area or a lamp with the Inject Wait property. For more information, refer to How to ...: Actions on the Control Panel  Linking a Control to a Device.

To perform the injection from a program

Include the Inject command in the program at  t = 0.000. When you create the program with the Program Wizard, the Inject command is included automatically. When you start the program, the injection is performed with the Volume and Position specified in the Sequence.

 Tip:

With the AS and AS50 autosamplers, sample loading and injection are two distinct events. Include a Load command in the program at t = 0.000 and then the Inject command. See  Autosampler Commands (AS/AS50) for details.

To perform the injection with an uncontrolled autosampler or with a syringe

You can also use an autosampler that is not controlled by Chromeleon for sample injection or you can inject the sample manually with a syringe. Chromeleon also waits for a signal before  the retention time clock is started. For example, with a hand-operated valve, the signal is returned after the valve has switched from Load to Inject. Thus, program execution is delayed until the injection is actually performed. This type of automatic injection requires connection of a Remote Input or remote start device via TTL or relay inputs and configuration of a Remote Inject device in the timebase. For details, see How to ...: Device Control Setting Up Remote Injection.