Commands and Tips for Third-Party Devices
Shimadzu LC-10A HPLC System: Program Example for the Dilution Injection Mode

 Tip: 

The Shimadzu devices support only programs that start at the time t = 0.000 min. If the program includes a command at a negative retention time, an abort error occurs during the Ready Check.

You can only set the injection mode if you have selected the Prep Loop option on the Sampler tab page in the Server Configuration program (SIL-10ADvp only).

The following program is an example for sample preparation with the Shimadzu SIL-10ADvp autosampler in Dilution injection mode:

; Pump settings:

 Pressure.LowerLimit = 2

 Pressure.UpperLimit = 150

 %A.Equate = "Water"

 Flow = 1.000

 %B = 0.0

; Detector settings:

 Mode = Dual

 Response = 2.00

 UV_VIS_1.Wavelength =  272

 UV_VIS_1.Step =  0.20

 UV_VIS_1.Average =  On

 UV_VIS_2.Wavelength =  254

 UV_VIS_2.Step =  0.20

 UV_VIS_2.Average =  On

; Settings for the Dilution InjectMode

; The commands below are necessary to allow the autosampler execute the desired actions:

 InjectMode = Dilution

 SourceVial = 30

; 20 µl of air is automatically drawn first, and then the calculate source volume, e.g., 200 µl, is drawn from position 30. Finally, 210 µl (= source volume + 10 µl) are delivered to the injection vial (defined position).

; Afterward, a Wash command is executed for the sample loop and the needle, using the settings for WashVolume and WashSpeed. For information about the general commands, refer to the previous topic:  Program Example for the Standard Injection Mode.

 DiluentVial = 51

; 20 µl of air is automatically drawn first, and then the calculated DilutVolume, e.g. 200 µl, is drawn from the position 51. Afterward, 410 µl (= DilutVolume + 10 µl) are then delivered to the injection vial (defined position).

 TotalVolume = 600

; Volume that shall be delivered to the injection vial after dilution (should exceed the injection volume).

 DilutFactor = 3

; Ratio between the diluent/sample ratio in the resulting mixture. If the total volume is 600 µl and if the dilution factor is 3, 200 µl are drawn from the SourceVial and 400 µl are drawn from the diluent vial and delivered to the injection vial.

 Position = 0

 Volume = 5

; The position of the injection vial is the position at which the sample and the reagent are mixed and from which injection is performed. You can specify the position in the PGM File or in the sample list of the sequence.

 MixRepeat = 3

 PrepVolume = 300

 Bubbling = Off

 PrepTime = 1.0

; Injection and data acquition:

0.000  UV.Autozero 

 Inject

 UV_VIS_1.AcqOn

 UV_VIS_2.AcqOn

3.000 UV_VIS_1.AcqOff 

 UV_VIS_2.AcqOff

 End

Tip:

It was observed that the PretDilute command delivers too much solvent to the target vial when used in the Dilution or Advanced injection mode. For a workaround to avoid this error, refer to:

Shimadzu LC-10A/LC-20A and LC-2010 HPLC Systems: Troubleshooting.

For other example programs, refer to:

 Shimadzu LC-10A HPLC System: Program Example for the Standard Injection Mode

 Shimadzu LC-10A HPLC System: Program Example for the Reagent Injection Mode

 Shimadzu LC-10A HPLC System: Program Example for the Advanced Injection Mode

For information about how to install the Shimadzu HPLC Systems, refer to Hardware Installation  Shimadzu LC-2010 HPLC System: Installation and  Shimadzu LC-10A/LC-20A HPLC System: Installation in the Administrator Help section.