Commands and Tips for Third-Party Devices
Shimadzu LC-10A HPLC System: Program Example for the Standard Injection Mode

 Tip: 

The Shimadzu devices support only programs that start at the time t = 0.000 min. It is not possible to perform commands at negative retention times. If the program includes a command at a negative retention time, an abort error occurs during the Ready Check.

The following program is an example for sample preparation with the Shimadzu SIL-10ADvp autosampler in Standard injection mode:

; Pump settings:

 Pressure.LowerLimit = 2

 Pressure.UpperLimit = 150

 %A.Equate = "Water"

 %B.Equate = "Methanol"

 Flow = 1.000

 %B = 80.0

; Detector settings:

 Mode = Dual

 Response = 2.00

 UV_VIS_1.Wavelength =  272

 UV_VIS_1.Step =  0.20

 UV_VIS_1.Average =  On

 UV_VIS_2.Wavelength =  254

 UV_VIS_2.Step =  0.20

 UV_VIS_2.Average =  On

; The following autosampler settings are valid for each InjectMode:

 WashMode = WashAfterSample

; Determines when the needle is washed (Options: NoWash, WashBeforeSample (before injection), WashAfterSample (after injection), Both (before and after injection)

 WashSpeed = 35

; Syringe speed when washing the needle

 WashVolume = 1

; Volume used for washing the sample loop

 SyringeSpeed = 15.0

; Speed with which the syringe draws the sample

 NeedleStroke = 52

; Indicates how deep the needle moves into the vial when drawing the sample

 Position = 0

 Volume = 5

; You can specify the vial position in the PGM File or in the sample list of the sequence.

 InjectMode = Standard

; Injection and data acquisition:

0.000 UV.Autozero 

 Inject

 UV_VIS_1.AcqOn

 UV_VIS_2.AcqOn

10.000  UV_VIS_1.AcqOff

 UV_VIS_2.AcqOff

 End

For other example programs, refer to:

 Shimadzu LC-10A HPLC System: Program Example for the Reagent Injection Mode

 Shimadzu LC-10A HPLC System: Program Example for the Dilution Injection Mode

 Shimadzu LC-10A HPLC System: Program Example for the Advanced Injection Mode

For information about how to install the Shimadzu HPLC Systems, refer to Hardware Installation  Shimadzu LC-2010 HPLC System: Installation and  Shimadzu LC-10A/LC-20A HPLC System: Installation in the Administrator Help section.