Shimadzu LC-10A HPLC System: Program Examples for the Advanced Injection ModeCommands and Tips for Third-Party Devices
Shimadzu LC-10A HPLC System: Program Examples for the Advanced Injection Mode
The following program is an example for mixing a sample with a reagent liquid using a Shimadzu SIL-10ADvp autosampler in Advanced injection mode. For a detailed list of the available Advanced pretreatment commands, refer to 
Shimadzu LC-10A/LC-20A HPLC System: Advanced Injection Mode.
Vials used:
Sample vial (no. SN) is an empty reaction vial
Sample liquid is contained in sample vial no. SN + 50 (upper half of the rack)
Reagent liquid is contained in reagent vial no. R101
Operation:
Transfuse 200 µl of the sample liquid and 200 µl of the reagent liquid in the sample vial.
Mix them three times.
After waiting for one minute, inject the sample according to standard injection procedure.
The commands on the left show the command syntax used in the Chromeleon PGM. On the right, the commands are translated into the corresponding Shimadzu syntax that is displayed on the system controller.
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Chromeleon PGM Syntax |
|
Shimadzu Syntax (displayed on the system controller) |
|
|
PretRinse |
|
RINSE |
RV,RS |
|
PretSet |
a1, sn, Add, 50 |
A1= |
SN+50 |
|
PretVial |
a1 |
VIAL |
A1 |
|
PretAir |
5 |
AIR.A |
5,SS |
|
PretNStrk |
|
N.STRK |
NS |
|
PretAspir |
200 |
ASPIR |
200,SS |
|
PretAir |
5 |
AIR.A |
5,SS |
|
PretVial |
|
VIAL |
SN |
|
PretNStrk |
|
N.STRK |
NS |
|
PretDisp |
208 |
DISP |
208,SS |
|
PretRinse |
|
RINSE |
RV,RS |
|
PretVial |
R101 |
VIAL |
101 |
|
PretAir |
5 |
AIR.A |
5,SS |
|
PretNStrk |
|
N.STRK |
NS |
|
PretAspir |
200 |
ASPIR |
200,SS |
|
PretAir |
5 |
AIR.A |
5,SS |
|
PretVial |
|
VIAL |
SN |
|
PretNStrk |
|
N.STRK |
NS |
|
PretDisp |
208 |
DISP |
208,SS |
|
PretRinse |
|
RINSE |
RV,RS |
|
PretVial |
|
VIAL |
SN |
|
PretMix |
3,200,200,ss,100 |
MIX |
3,200,200,SS,100 |
|
PretRinse |
|
RINSE |
RV,RS |
|
PretWait |
1.0 |
WAIT |
1.0 |
|
PretGotoF0 |
|
GOTO F0 |
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Important:
Chromeleon does not perform any checks to ensure that the pretreatment program is correct. Therefore, it is in the responsibility of the user to make sure that the PGM is reasonable and correct, and that the pretreatment command sequence is consistent.
Care must also be taken when using pretreatment programs for different autosampler types. Split-loop (e.g., SIL-10A) and pulled-loop autosamplers (e.g., SIL-10AXL or SIL-10ADvp) use different injection procedures and may thus need programs with different command sequences. Therefore, it is recommended to write a separate program for each sampler type.
For other example programs, refer to:
Shimadzu LC-10A HPLC System: Program Example for the Standard Injection Mode
Shimadzu LC-10A HPLC System: Program Example for the Dilution Injection Mode
Shimadzu LC-10A HPLC System: Program Example for the Reagent Injection Mode