Property

Range/Values

Description

AutoActivateLamp

Off/On

If set to On, the lamp is automatically turned on right after power-up.

BaselineBehavior

Append, Free, Zero

Determines how the baseline behaves after the wavelength or photomultiplier gain has changed:

Append: Resets the baseline to the previous position.

Free: No adjustment, shifts may occur in the baseline position.

Zero: Sets the baseline to 0 LU (luminescence units).

Dark_Pmt²

0 - 999999

Last value (in counts) for the dark current of the photomultiplier (read-only). The value should not exceed 10000 counts.

Dark_Reference²

0 - 999999

Last value (in counts) of the dark current for the reference diode (read-only). The value should not exceed 5000 counts.

DetectionMode³

Fluorescence,
Phosphorescence

Specifies the operating mode.

 Tip:

The property can be set to Phosporescence only for detectors with a firmware version A.03.6x and higher. In addition, ScanMode must be set to Off.

Emission_Deviation_
Absolute²

-12.000 - 12.000nm

Correction value for the emission wavelength as established during Test_Calibration as the optimum value (read-only).

Emission_Deviation_
Correction²

-12.000 - 12.000nm

Currently used correction value for the emission wavelength (read-only).

EmScanDuration

0 - 99999

Indicates the time in seconds needed to scan an emission spectrum (read-only). (This property can be used only if ScanMode=Emission.)

 Tip: 

To perform meaningful scans, make sure that this time is less than the width of the smallest peak. A warning appears if the time exceeds one of the peak widths defined in the QNT Method.

EmScanStepSize

1 - 20

Specifies the wavelength increments at which the detector scans the emission spectrum. (You can set this property only if ScanMode = Emission.)

EmScanWhen

None, Apex, AllInPeak, All, AllWithoutSignals

Determines when the scans of an emission spectrum are taken. (You can set this property only if ScanMode = Emission.)

For more information, refer to  Scan Modes for the Fluorescence Detector.

Excitation_Deviation_
Absolute²

-12.000 - 12.000nm

Correction value for the excitation wavelength as established during Test_Calibration as the optimum value (read-only).

Excitation_Deviation_
Correction²

-12.000 - 12.000nm

Currently used correction value for the excitation wavelength (read-only).

ExScanDuration

0 - 99999

Indicates the time in seconds needed to scan an excitation spectrum (read-only). (This property can be used only if ScanMode=Excitation.)

 Tip: 

To perform meaningful scans, make sure that this time is less than the width of the smallest peak. A warning appears if the time exceeds one of the peak widths defined in the QNT Method.

ExScanStepSize

1 - 20

Specifies the wavelength increments at which the detector scans the excitation spectrum. (You can set this property only if ScanMode = Excitation.).

ExScanWhen

None, Apex, AllInPeak, All, AllWithoutSignals

Determines when the scans of an emission spectrum are taken. (You can set this property only if ScanMode = Excitation.)

For more information, refer to  Scan Modes for the Fluorescence Detector.

FitSpectralRange³

No/Yes

When set to Yes, the spectral ranges have to be set in such a way that there is always a difference of at least 25 nm between the shortest emission wavelength and the longest excitation wavelength. This avoids that emission is disturbed by stray light.

 Tip:

A warning appears if, in a program, the distance between the longest excitation wavelength and the shortest emission wavelength is less than 25 nm.

HighPowerFlashes

0 - 99999

Number of lamp flashes at high power (in thousands). HighPowerFlashes.Value indicates the current value. Set the HighPowerFlashes.UpperLimit to 0 to disable the associated limit test.

LampEnergyReference

Off/On

Set to On to compensate for variations in light intensity of the lamp, using a reference diode. This may improve the signal-to-noise ratio.

LampFlashRate

Standard, Economy

Specifies the rate at which the lamp pulses:

Economy: 74 Hz - prolongs the lamp life.

Standard: 296 Hz - increases the detector sensitivity.

LampOnOnlyDuringRun

No/Yes

Specifies the lamp state when no analysis is running:

Yes: Turns the lamp off after each analysis.

No: The lamp keeps burning after each analysis.

LampRequired

No/Yes

Specifies whether the lamp is required for the next analysis:

Yes: If the lamp is turned off, the detector is in NotReady state.

No: The configuration includes several detectors; the fluorescence detector is not required for the next analysis.

LowPowerFlashes

0 - 99999

Number of lamp flashes at low power (in thousands). LowPowerFlashes.Value indicates the current value. Set the LowPowerFlashes.UpperLimit to 0 to disable the associated limit test.

PeakWidth³

<0.005, >0.005, >0.010, >0.025, >0.050, >0.100, >0.200, >0.400

Specifies the detector's response time (in minutes).

PeakWidthPD³

<0.005, >0.005, >0.010, >0.025, >0.050, >0.100, >0.200, >0.400

Select from which signal width the peak shall be recognized. This setting plus the Threshold (see below) determine the internal peak recognition of the device firmware. For EmScanWhen or ExScanWhen = Apex or AllnPeak, scanning is performed only if a peak is recognized in the first channel.

Specifies the expected peak width (in minutes) for the internal peak detector.

 Tip: 

The property may be set only once and only at retention times <0.000 min.

PhosphorescenceDelay³

0.00 - 5000.00

Specifies the time (in µs) for which the lamp must be on before the detector starts recording data.

 Tip:

This property can be set only if DetectionMode = Phosphorescence.

PhosphorescenceGate³

20.00 - 5000.00

Specifies the time (in µs) during which data is recorded after the lamp was on.

 Tip:

This property can be set only if DetectionMode = Phosphorescence.

PMTGain³

0 - 18

Specifies the photomultiplier gain. (A typical setting is between 10 and 15.) Each step approximately doubles the gain. Use lower values for very high concentrations. Higher values may improve the signal-to-noise ratio.

Polarity

Positive, Negative

Specifies the signal polarity. Set the polarity to the expected signal polarity.

 Tip:

The property may be set only once and only at retention times <0.000 min.

RemainingLifetime

0 - 100

Remaining lifetime of the lamp (in percent). RemainingLifetime.Value indicates the current percentage. Set the RemainingLifetime.UpperLimit to 0 to disable the associated limit test.

ScanEndEmWavelength

281 - 900

Specifies the emission wavelength at which the scan should end. (This property is used only if ScanMode = Emission.) In addition, the following must be true:

ScanEndEmWavelength >ScanStartEmWavelength and >ExWavelength

ScanEndExWavelength

201 - 700

Specifies the excitation wavelength at which the scan should end. (This property is used only if ScanMode = Excitation.) In addition, the following must be true:

ScanEndExWavelength >ScanStartExWavelength and <EmWavelength

ScanMode³

Off, Emission Excitation

Specifies the scan mode. For more information, refer to  Scan Modes for the Fluorescence Detector.

 Tip:

Changing the mode takes about 5-10 seconds. During this time, the detector is in NotReady state.

ScanStartEmWavelength

280 - 899

Specifies the emission wavelength at which the scan should start. (This property is used only if ScanMode = Emission.) In addition, the following must be true:

ScanStartEmWavelength <ScanEndEmWavelength and >ExWavelength

ScanStartExWavelength

200 - 699

Specifies the excitation wavelength at which the scan should start. (This property is used only if ScanMode = Excitation.) In addition, the following must be true:

ScanStartExWavelength <ScanEndExWavelength and <EmWavelength

Threshold

0.0001 - 1^5

Select from which signal height in LU (Luminescence Units) the peak shall be recognized. This setting plus PeakWidthPD (see above) determine the internal peak recognition of the device firmware. For EmScanWhen or ExScanWhen = Apex or AllnPeak, scanning is performed only if a peak is recognized in the first channel.

Specifies the height of the smallest expected peak in LU (Luminescence Units). The detector ignores all peaks below this threshold value.

Command

Description

Abort_Calibration

Aborts the detector's internal calibration procedure.

 Tip: 

This command can be performed only when the instrument is performing a calibration.

Adjust_Calibration

After the internal calibration of the detector, you can use this command to update the internal calibration settings, based on the test. That means that the measured Excitation_Deviation_Absolute value is assigned to Excitation_Deviation_Correction and the measured Emission_Deviation_Absolute value to Emission_Deviation_Correction.

 Tip:

This command can be used only if an internal calibration was successfully performed via the Test_Calibration command.

DarkCurrentTest

Performs a dark current test. The results of the last dark current test are saved as properties (Dark_Reference and Dark_Pmt, see above).

 Tip:

This command can be performed only if the detector is idle. In addition, the lamp must be on.

PmtTest

Calculates an appropriate photomultiplier gain, based on the settings.

 Tip: 

This command can be performed only if the detector is idle.

Scan

Saves the spectrum scan.

 Tip: 

This command can be performed only during data acquisition. In addition, verify that ScanMode is not set to Off and that ScanWhen is set to All.

Test_Calibration

Starts the detector's internal calibration procedure and updates the associated properties with the results.

 Tip:

This command can be performed only if the detector is idle. In addition, the lamp must be on.

Property

Min.

Max.

Description

EmWavelength

200 to 700 nm,

Off, ZeroOrder

Emission wavelength in nm

Off: No data is recorded on this channel. This improves the signal-to-noise ratio on the other channels because more single values are averaged.

ZeroOrder: The monochromator grating serves as mirror. The entire emission spectrum is recorded on the channel.

 Tip:

The EmWavelength must be longer than all used excitation wavelengths. In addition, it must be identical for all channels in programs with ScanMode = Excitation.

ExWavelength

200 to 700 nm,

Off, ZeroOrder

Excitation wavelength in nm

Off: No data is recorded on this channel. This improves the signal-to-noise ratio on the other channels because more single values are averaged.

ZeroOrder: The monochromator grating serves as mirror. The entire lamp spectrum is used for excitation. Overlaps with the emission wavelength result in increased stray light and thus, in increased background noise.

 Tip: 

The ExWavelength must be shorter than all used emission wavelengths. In addition, it must be identical for all channels in programs with ScanMode = Emission.