Commands and Tips for Third-Party Devices
Agilent 1100/1200 HPLC System: Program Examples for the Fluorescence Detector

The following program example applies to an HPLC 1100 system:

1. One channel, no spectra acquisition

Set the operating mode to ScanMode = Off.

Fluorescence.PeakWidth = 0.200

 ScanMode = Off

 Emission_1.ExWavelength =  335

 Emission_1.EmWavelength =  390

 Emission_1.Step =  Auto

 Emission_1.Average =  On

0.000  Wait Sampler.Ready and Fluorescence.Ready

 Inject

 Emission_1.AcqOn

2.000  Emission_1.AcqOff

 End

2. Several channels, no spectra acquisition

Set the operating mode to ScanMode = Emission or Excitation. In addition, set EmScanWhen (and/or ExScanWhen) to None.

 Note:  

Set ExWavelength (or EmWavelength, respectively) to Off for all channels for which data acquisition will not be performed. The other wavelength must be identical for all channels.

Fluorescence.PeakWidth = 0.200

 ScanMode = Emission

 EmScanWhen = None

 PeakWidthPD = 0.200

 Threshold = 1.000

 Emission_1.ExWavelength =  335

 Emission_1.EmWavelength =  390

 Emission_1.Step =  Auto

 Emission_1.Average =  On

 Emission_2.ExWavelength =  335

 Emission_2.EmWavelength =  450

 Emission_3.ExWavelength =  Off

 Emission_3.EmWavelength =  390

 Emission_4.ExWavelength =  Off

 Emission_4.EmWavelength =  390

0.000  Wait Sampler.Ready and Fluorescence.Ready

 Inject

 Emission_1.AcqOn

 Emission_2.AcqOn

2.000  Emission_1.AcqOff

 Emission_2.AcqOff

 End

3. Peak-dependent spectra acquisition

Set the operating mode to ScanMode = Emission (or Excitation). In addition, set EmScanWhen (or ExScanWhen, respectively) to Apex (only at the peak maximum) or to AllInPeak (= all spectra during the peaks).

 Tip:  

Verify that the settings for PeakWidthPD and Threshold match the peaks recorded on the first channel (Emission_1).

 Fluorescence.PeakWidth = 0.200

 ScanMode = Emission

 EmScanWhen = Apex

 PeakWidthPD = 0.200

 Threshold = 1.000

 Emission_1.ExWavelength =  335

 Emission_1.EmWavelength =  390

 Emission_1.Step =  Auto

 Emission_1.Average =  On

 Emission_2.ExWavelength =  Off

 Emission_2.EmWavelength =  390

 Emission_3.ExWavelength =  Off

 Emission_3.EmWavelength =  390

 Emission_4.ExWavelength =  Off

 Emission_4.EmWavelength =  390

0.000  Wait Sampler.Ready and Fluorescence.Ready

 Inject

 Emission_1.AcqOn

2.000  Emission_1.AcqOff

 End

4. User-defined spectra acquisition

Set the operating mode to ScanMode = Emission (or Excitation). In addition, set EmScanWhen (or ExScanWhen, respectively) to All:

Trigger DoScan Emission_1.Signal > 10.000

 Scan

EndTrigger

 Fluorescence.PeakWidth = 0.200

 ScanMode = Emission

 EmScanWhen = All

 Emission_1.ExWavelength =  335

 Emission_1.EmWavelength =  390

 Emission_1.Step =  Auto

 Emission_1.Average =  On

 Emission_2.ExWavelength =  340

 Emission_2.EmWavelength =  390

 Emission_2.Step =  Auto

 Emission_2.Average =  On

 Emission_3.ExWavelength =  Off

 Emission_3.EmWavelength =  390

 Emission_4.ExWavelength =  Off

 Emission_4.EmWavelength =  390

0.000  Wait Sampler.Ready and Fluorescence.Ready

 Inject

 Emission_1.AcqOn

 Emission_2.AcqOn

1.000  Scan

2.000  Emission_1.AcqOff

 Emission_2.AcqOff

 End

The DoScan Trigger determines that a fluorescence spectrum is recorded when the signal of the Emission_1 channel exceeds 10.

Also, refer to  Agilent 1100/1200 HPLC System: Scan Modes for the Fluorescence Detector.

For information about the commands for fluorescence detector control, refer to  Agilent 1100/1200 HPLC System: Fluorescence Detector.