Commands and Tips for Third-Party Devices
Agilent 1100/1200 HPLC System Fluorescence Detector: Tips for Lamp Commands

Chromeleon supports several commands for defining the state of the Xenon lamp of the fluorescence detector that is installed in the 1100 or 1200 HPLC System. Depending on the situation, you have to use one of the first three settings in your PGM Files:

1. No data acquisition

The fluorescence detector is available but data acquisition is not performed for fluorescence data (e.g., only UV data are recorded):

LampRequired = No

Lamp = Off

LampOnOnlyDuringRun = No

 Tip:  

If the PGM File contains an Emission_n.AcqOn command, the PGM File or the batch does not pass the Ready Check.

2. The lamp is turned on only if required

The fluorescence detector is used for data acquisition. However, the lamp is turned on only if required.

LampRequired = Yes

Lamp = On

LampOnOnlyDuringRun = Yes

The lamp is turned off after each sample. However, the instrument remains ready for operation after the analysis. The lamp is automatically turned on when the next sample is started.

 Tip:  

It may be necessary to turn on the lamp (i.e., to issue a Lamp=On command) manually before starting the analysis of the first sample; e.g., if the detector is in NotReady state.

3. The lamp is burning permanently

The fluorescence detector is used for data acquisition. The lamp is burning permanently.

LampRequired = Yes

Lamp = On

LampOnOnlyDuringRun = No

 Tip:  

This reduces the lamp's life cycle. However, analysis is performed more quickly because the lamp is warmed up only once (at the very beginning); it does not need to warm up before each sample (see 2. above).

4. Not allowed

You should not combine the following commands:

LampRequired = Yes

Lamp = Off

LampOnOnlyDuringRun = No

With this sequence of commands, the Ready Check would fail. The detector remains in NotReady state.

For more information, refer to:

 Agilent 1100/1200 HPLC System: Scan Modes for the Fluorescence Detector

 Agilent 1100/1200 HPLC System: Example Programs for the Fluorescence Detector

For information about the commands for fluorescence detector control, refer to  Agilent 1100/1200 HPLC System: Fluorescence Detector.