Installing and Controlling Thermo Scientific Devices
Finnigan/TSP FL2000 and FL3000 Fluorescence Detectors: Installation

 

Device Type:

Fluorescence Detector

Device Driver:

TSP FL2000 Fluorescence Detector or
TSP FL3000 Fluorescence Detector

Hardware Options
Supported:

Excitation: 200 to 650 nm

Emission: 200 to 650 nm
200 to 800 nm with Extended Range Photomultiplier

The following photochemical effects are supported for detection:

Only FL3000: Excitation and emission wavelength scanning

 Note:  

The FL2000 detector was tested with firmware version 3.11.

Restrictions (FL3000):

  • Scanning may disturb the chromatogram. Therefore, we recommend that you do not scan during a quantitative analysis.

  • Scans can be recorded during a program run only.

  • Any spectra are saved in the detector RAM until the end of the run. If the detector is running out of RAM during a run, any following spectra scans of the program will be skipped, and the related error messages are displayed in the Audit Trail.

  • You cannot start a scan until the previous baseline scan has finished.

 

What is required?

License:

Timebase Class 1

Connection:

A free RS-232 port and the following cable are required:

RS-232 cable (Dionex part no. 8914.0150A) (for cable details, refer to  TSP Cable)

Hardware Installation

Device Connection:

Connect the RS-232 cable to a COM port on the Chromeleon Server PC.

 Tips:  

  • You cannot control the TSP FL2000/FL3000 detectors via a UCI Universal Chromatography Interface or a USB-to-RS232 adapter. To control the detectors, use a free RS-232 port on the PC. If more RS-232 ports are needed, install an RS-232 interface card. (For example, refer to Installing PC Plug-In Cards  Installing the Serial RS-232 Interface Card (Meilhaus).)

  • When you install the detector in Chromeleon, verify that the Start and Stop inputs are not connected. This will ensure error-free control of the device.

  • The controller box SN4000 is not required for operating the detector with Chromeleon. Disconnect the SN4000, if necessary.

Device Settings:

The following settings are supported:

 Baud rate 9600

 Handshake Off

 Parity None

 Data bits 8

 Stop bits 1

 

Software Installation

  1. For details about how to add timebases and device drivers to the Server Configuration, refer to Adding, Configuring or Deleting Devices. When you add a driver to the timebase, the configuration dialog or wizard opens automatically.

  2. The following settings are required:

On the General tab page, select the RS-232 port. In addition, disable the Demo Mode.

On the Options tab page, enable or disable control of the extended range photomultiplier tube (PMT).

On the Signals tab page, verify that data acquisition on the emission channel is enabled. (The default settings are: Signal name: Emission; Unit: FU (Fluorescence unit), and Factor: 6.25 * 10^-5 (=1/16.000.)

The Error Levels tab page classifies the severity of any errors that occur. It is generally not necessary to change the default settings .If you want to use the detector to measure the Chemiluminescence, record the signal with the lamp turned off. In this case, set the error message "The lamp can't be switched off during data acquisition." to Ignore.

For information about the settings, click the Help button on a page or press F1.

Application

The User Help section provides information about the commands and properties supported by the fluorescence detectors; refer to Commands and Tips for Thermo Scientific Devices

 Finnigan/TSP FL2000 and FL3000 Detectors

 Finnigan/TSP FL2000 and FL3000 Detectors: Different Names in Firmware and Chromeleon

Troubleshooting

For information about troubleshooting, refer to  Finnigan/TSP FL2000/FL3000: Troubleshooting.

Further Information

 Tip:

For more installation details, refer to the manual for the instrument.

For an overview of the Thermo Scientific/Finnigan/ThermoQuest/TSP instruments for which device drivers are available in Chromeleon, refer to  Thermo Scientific.