Property

Min

Max

Description

AnOut_1_Offset, AnOut_2_Offset

-50; -20; -10; -5; -2; -1; 0; 1; 2; 5; 10; 20; 50 %FS

Offset on the analog output. (This value is added to the associated measured value.)

AnOut_1_Range, AnOut_2_Range

500; 200; 100; 50; 20; 10; 5; 2; 1; 0,5; 0,2; 0,1; 0,05; 0,02; 0,01 FUFS

Output range for the analog output.

DetectionMode

Fluorescence, Phosphorescence

Operating mode of the detector.

Lamp

Off

On

Turns the lamp on or off. (Note: It is not possible to turn the lamp off during data acquisition. Also, it is not possible to start data acquisition if the lamp is off.)

LampFlashRate

20 Hz

100 Hz

Pulse rate of the xenon lamp (20 Hz prolongs the lamp's life cycle; 100 Hz increases the sensitivity for the measurements).

NumberOfScans²

2 - 32 (in 2 nm
increments)

This property is available only for the FL3000.

It specifies how often the monochromator repeats each scan for averaging.

PmtVoltage

0, 500, 600, 700, 800, 900, 1000 V

Specifies the voltage applied to the photomultiplier tube (PMT). (The PMT's sensitivity increases proportional to the applied voltage. However, higher voltages shorten the PMT's life cycle.)

ScanEndEm
Wavelength²

200 - 650 nm
(or 800 nm;
in 2 nm increments)

This property is available only for the FL3000.

Emission wavelenth at the end of the scan. (Ignored for ScanMode = Excitation)

The difference between ScanStartEmWavelength and ScanEndEmWavelength must be a multiple of 10.

ScanEndEx
Wavelength²

200 - 650 nm
(in 2 nm increments)

This property is available only for the FL3000.

Excitation wavelength at the end of the scan
(Ignored for ScanMode = Emission)

The difference between ScanStartEmWavelength and ScanEndEmWavelength must be a multiple of 10.

If ScanMode=Delta, the differences between the emission and the excitation scan ranges must be identical:
(ScanEndEmWavelength - ScanStartEmWavelength) equals (ScanEndExWavelength -ScanStartExWavelength).

ScanMode

Excit.

Emission

This property is available only for the FL3000.

Scan mode:

• Excitation: The emission wavelength is constant; the excitation monochromator scans the wavelengths.

• Emission: The excitation wavelength is constant; the emission monochromator scans the wavelengths.

• Delta: Both monochromators perform the scans simultaneously; the difference between the wavelengths is constant.

ScanStartEm
Wavelength²

200 - 650 nm
(or 800 nm;
in 2 nm increments)

This property is available only for the FL3000.

Emission wavelength at the beginning of the scan (ignored for ScanMode = Excitation)

ScanStartEx
Wavelength²

200 - 650 nm
(in 2 nm increments)

This property is available only for the FL3000.

Excitation wavelength at the beginning of the scan (ignored for ScanMode = Emission)

ScanStepSize

2, 4, 8, 16 nm

This property is available only for the FL3000.

Specifies the step for the wavelength scan.

Command

Description

Scan

This property, which is available only for the FL3000, starts the scan for the emission and/or excitation wavelength.

Scanning is possible only while a PGM File is running.

During the run of a PGM File, manual scanning is possible only if the detector provides enough storage capacity.

A scan can start only after the previous baseline scan is finished (see ScanAutozero).

 Tip:

The chromatogram is disturbed during the scan.

ScanAutozero

This property, which is available only for the FL3000, starts the baseline scan for the emission and/or excitation wavelength.

A baseline scan can start only after the previous baseline scan is finished.

Property

Min

Max

Description

AutozeroOnWlChange

Off, On

If the setting is On, autozeroing is performed each time the wavelength (see EmWavelength and ExWavelength) is entered.

 Tip:  

Autoszeroing is performed each time the wavelength is changed, even if the new value is the same as the previous one.

EmWavelength

200 - 650
(or 800;
in increments of 2)

Emission wavelength in [nm].

ExWavelength

200 - 650
(in increments of 2)

Excitation wavelength in [nm].

Response

0.0, 0.1, 0.2, 0.5, 1.0, 2.0, 5.0, 10.0

Specifies the response time in [s] for the detector (see Response).

Signal.UpperLimit
Signal.LowerLimit

-1000

1000 FU

Upper and lower signal limit.

 Tip:

If the actual value is above the upper limit or below the lower limit, Chromeleon aborts the batch and starts emergency handling.

Signal.Value

-1000

1000 FU

Indicates the current signal value.