Device Type:

Autosampler

Device Driver:

Dionex HPLC: UltiMate 3000

OAS-3X00TXRS

Hardware Options
Supported:

• Configuration 1 (OAS-3300TXRS): Standard Open Autosampler with 3 drawer stack cooler

• Configuration 2 (OAS-3600TXRS): Standard Open Autosampler with 6 drawer stack cooler (only well plates)

For detailed information about the available configurations, refer to the operating instructions for the autosampler.

Restrictions:

• Sharing modules between timebases is not supported

• Manual injection is not supported

• Programs must be created using the PGM Wizard. Manual editing in the PGM Editor is restricted to a few commands.

• Demo Mode is not supported.

• Teaching of reference positions, as well as changes of the syringe type and tray type, must be performed by using the Control Terminal.

• The autosampler cannot be controlled using the RS-232 ports of an UCI Universal Chromatography Interface.

License:

Timebase Class 1

Connection:

There are two possible types of communication between the Chromeleon Server PC and the autosampler: Ethernet (LAN, default setting) or serial (RS-232) communication.

Ethernet (LAN): A free Ethernet port and the following cable are required:

• Ethernet LAN cable to connect the module to the network via an Ethernet hub or switch

  –OR–

• Standard crossover cable to connect the autosampler directly to the Server PC.

Serial: A free COM port and the following cable are required:

• RS-232 cable (not provided by Thermo Fisher Scientific)

Tip:

Thermo Fisher Scientific recommends to connect the instrument via LAN.

The following cables are required to connect the autosampler with a pump:

• OAS-3x00 interconnect cable to transmit the Inject Response signal between the autosampler and the pump. The cable (Dionex part no. 6043.0004) is shipped with the instrument.

• Signal synchronization cable to synchronize the injection command with the pump strokes. The signal synchronization cable is available in two different types designed for the following pump modules:

  UltiMate 3000 RS, SD or BM pumps: Signal synchronization cable, Dionex part no. 6043.0001.

  LPG-3400XRS pumps: Signal synchronization cable, Dionex part no. 6043.0002.

The following cable is required for a UltiMate 3000 system consisting of a

OAS-3x00XRS, LPG-3400XRS and a Corona or Coulochem detector:

• Synchronization cable, Dionex part no. 6043.0003. The cable is shipped with the Corona or Coulochem detector, respectively.

In addition, the following RS-232 cables are required for connection of the autosampler with the Chromeleon Server PC:

• RS-232 synchronization cable to connect the autosampler to the RS-232 port on the Chromeleon computer (shipped with the instrument, Dionex part no. 6043.0005).

• RS232-USB Interface cable required in addition to the RS-232 synchronization cable, if an RS-232 port is not available on the computer (shipped with the instrument, Dionex part no. 6073.2000).

For details on how to connect the autosampler to an UltiMate 3000 RS, SD, BM or XRS pump, refer to UltiMate 3000 Series OAS-3x00TXRS Autosampler: Connecting the Autosampler.

Software Prerequisites:

The Instrument Controller Components package (ICC), version 1.6.0.5 or later is required. The package is provided on the Chromeleon DVD and is installed automatically with Chromeleon 6.80 SR11d and later.

Firmware Requirements:

The minimum required firmware version is 4.1.5. Tested with firmware version 4.1.5.

Control Panel:

To control the instrument, use

• panel tabsets (refer to How to:  Controlling Devices from the Panel Tabset)

  OR

• a suitable panel, for example, UltiMate_3000_OASXRS.pan, from the
  Dionex Templates\Panels\Dionex_LC folder in the Browser.

Device Connection:

To connect the autosampler with the Chromeleon Server PC

There are two possible types of communication between the Chromeleon Server PC and the autosampler: Ethernet (LAN) for communication via TCP/IP or serial via RS-232 communication.

1. Ethernet (LAN) communication

By default, the autosampler is configured for communication via TCP/IP. The default IP address for the OAS-3x00TXRS is as follows:

To establish LAN communication, proceed as follows:

Establish the LAN connection between autosampler and Server PC by connecting the instrument to an Ethernet switch or hub using the standard Ethernet LAN cable shipped with the instrument.

Alternatively, you can use a Standard crossover cable to connect the autosampler directly to the Server PC.

Tip:

For better reliability, Thermo Fisher Scientific recommends connecting the autosampler to a separate device LAN and not to the Office LAN. Note that an additional network card is required when using a device LAN.

For information about how to set up the TCP/IP connection, also refer to Connect Modules via TCP/IP (LAN) and the related subtopics.

2. Serial (RS-232) communication

As an option, serial communication via RS-232 can be set up for the autosampler. However, LAN communication is the recommended type of communication.

To establish serial communication, proceed as follows:

1. Use a standard null modem cable to connect the SER1 port on the autosampler's rear panel to a free COM port on the Chromeleon Server PC.

2. In the Windows Device Manager (under Windows -> Control Panel -> Device Manager -> Ports), verify that the COM port settings are as follows:

Device Settings:

If required, communication settings for the autosampler can be changed by using the autosampler's Control Terminal.

To set the communication protocol

Switch the autosampler from Ethernet (LAN) to serial (RS-232) communication or vice versa, if required. The current setting (SER or LAN) is shown during boot-up.

1. On the Job Queue screen, select Menu by pressing the F1 key.

2. From the Menu screen, rotate the outer knob to move the cursor bar (highlight) to Setup.

3. Press F3 to access the extended user level options.

4. Press the Enter button to select Setup.

5. Rotate the outer knob to move the cursor bar (highlight) to Communication.

6. Press the Enter button to select Communication. The Communication screen appears with the Comm Port row selected.

7. Press the Enter button to select CommPort. Rotate the outer knob to select one of the following:

   • To specify serial communication, select SER1.

   • To specify Ethernet communication, select LAN.

8. Press the Enter button to confirm your selection.

To set the IP address, subnet masks, or gateway address

Depending on the LAN environment and number of autosamplers used, you may want to change the IP address or related settings.

1. Go to the Communication menu as described above.

2. Do one or more of the following:

   • Set the IP address under IpAddr1 to IpAddr4.

   • Set the subnet mask under SubNet1 to Subnet4.

   • Set the gateway address under StdGateway1 to StdGateway4.

Note:

If you are controlling more than one OAS-3x00TXRS autosampler via LAN from the Chromeleon Server PC, each autosampler must have a unique IP address. The IP address for the OAS-3x00TXRS is 172.16.1.200, where the fourth identifier (.200) is a unique integer value for each autosampler connected to the PC.

For detailed information about how to use the Control Terminal, refer to the OAS-3x00TXRS operating manual.

Hardware Synchronization:

Typically, the autosampler is operated with one of the following UltiMate 3000 devices:

• UltiMate 3000 LPG-3400XRS pump

• UltiMate 3000 RS, SD or BM pump

• Ultimate DAD-3000(RS), VWD-3x00, FLD-3x00(RS),

• Corona CAD or Coulochem III Electrochemical detector

Connect the autosampler with an UltiMate 3000 RS, SD, BM or XRS pump and/or Corona or Coulochem detector, as described in topic UltiMate 3000 Series OAS-3x00TXRS Autosampler: Connecting the Autosampler.

After successful device connection, you have to configure synchronization settings on the Control Terminal of the autosampler to enable:

• transmission of the Inject Response signal between the autosampler and the pump

• synchronization of the injection command with the pump strokes

For details, see sections Inject Response and Pump Stroke Synchronization below.

Inject Response

To ensure that the inject response signal is transmitted properly, the OUT signal settings for the autosampler have to be configured as follows:

1. Navigate to the Setup menu as described above under Device Settings.

2. Proceed to Objects and then to Out Signals.

3. Select Injected and verify that Destination is set to SW-Out1.

Pump Stroke Synchronization

In pump stroke synchronization, the pump strokes are synchronized with the injection command of the autosampler. Synchronization ensures that all injections are performed at the same phase of the pump cycle; this enhances the retention time precision for gradient applications.

To enable pump stroke synchronization, you have to define the pump unit used for synchronization on the Open Autosampler OAS-3x00TXRS page (option Pump Link (LPG only)) in the Chromeleon Server Configuration.

Exception: When using a LPG-3400XRS pump, set Pump Link (LPG only) to None.

Then change to the autosampler's control terminal to specify the synchronization signal:

1. Navigate to the Sync Signals menu (via Menu -> Setup -> Objects -> Sync Signals).

2. Select Inject2 and set Source to TTL-In1.

3. Navigate to the Events menu (Menu -> Setup -> Objects -> Events).

4. Verify for TTL-In1 that Active State is set to Low.

To disable pump stroke synchronization:

If the pump does not support pump stroke synchronization or no synchronization is needed, you can disable pump stroke synchronization as follows:

1. On the Open Autosampler OAS-3x00TXRS configuration page in the Server Configuration, set Pump Link (LPG only) to None.

2. Then change to the autosampler's control terminal and navigate to the Sync Signals menu (via Menu -> Setup -> Objects -> Sync Signals).

3. Select Inject2 and set Source to Immediat.