Thermo Scientific TriPlus Sampler: Creating a Program for Liquid InjectionsCommands and Tips for Thermo Scientific Devices
Thermo Scientific TriPlus Sampler: Creating a Program for Liquid Injections
Follow the steps below to create a program for liquid injections:
Use the PGM Wizard to create a program for the timebase.
Add all relevant settings for the sampler in the PGM Editor. Use the example program below for assistance.
The settings must appear before the first time step, i.e., before t = 0.000 min.
Add a Wait Sampler.Ready AND GC.Ready statement directly before the Inject command unless not already included by the PGM wizard.
Example Program
The following is an example PGM for performing GC liquid injections. It is designed as a template and includes all parameters available for the TriPlus As sampler type. You may copy this program and edit parameters according to your specific analysis tasks.
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; ======================================================================= ; Specify the injection mode ; ======================================================================= |
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InjectMode |
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InternalStandardPost |
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; ======================================================================= ; Specify enrichment parameters (only required if InjectMode is 'Enrichment' or ;' EnrichmentNeedleSolvent'). ; Set LoadsPerInject = 1 for all injection modes except 'Enrichment' and ; 'EnrichmentNeedleSolvent' ; If LoadsPerInject =1, you do not need to specify LoadRepititionTime ; ======================================================================= |
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LoadsPerInject |
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1 |
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LoadRepetitionTime |
= |
10 [s] |
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; ======================================================================= ; General sampling parameters ; ======================================================================= |
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FillStrokes |
= |
2 |
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AirVolume |
= |
1.0 [µl] |
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FillVolume |
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2.0 [µl] |
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DownSpeed |
= |
120 [mm/s] |
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SampleDepth |
= |
Bottom |
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DrawSpeed |
= |
NotViscousDefault |
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PullupDelay |
= |
NotViscousDefault |
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DelayAfterBubbleElimination |
= |
NotViscousDefault |
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BubbleEliminationSpeed |
= |
63.0 [µl/s] |
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DelayBetweenStrokes |
= |
1.0 [s] |
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; ======================================================================= ; Specific sampling parameters ; The following parameters must only be set if the injection mode is ; 'InternalStandardPost' or 'InternalStandardDouble'; otherwise, parameters can be omitted. ; ======================================================================= |
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IntStdVolume |
= |
1.0 [µl] |
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IntStdAirGap |
= |
1.0 [µl] |
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IntStdPosition |
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96 |
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; ======================================================================= ; The following parameters must only be set if the injection mode is ; 'SolventFlushPost', 'SolventflushDouble', 'NeedleSolventFlush' or ; 'EnrichmentNeedleSolvent'; otherwise, parameters can be omitted. ; ======================================================================= |
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SolventFlushVolume |
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1.0 [µl] |
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SolventFlushAirGap |
= |
1.0 [µl] |
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SolventFlushPosition |
= |
1 |
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SolventFlushDepth |
= |
50 [%] |
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; ====================================================================== ; Injection parameters ; ======================================================================= |
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Injector |
= |
InjectorA |
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GCStartMode |
= |
Standard |
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PreInjDelayTime |
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5.0 [s] |
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PostInjDelayTime |
= |
5.0 [s] |
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InjectSpeed |
= |
SpeedForStandardDepth |
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InjectDepth |
= |
Standard |
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; ======================================================================= ; The following injection parameters must only be set if InjectSpeed ; is 'LVRamp'; otherwise, parameters can be omitted. ; ======================================================================= |
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LVInitialInjectSpeed |
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10.0 [µl/s] |
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LVInitialInjectDepth |
= |
17 [mm] |
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LVInitialInjectTime |
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200 [s] |
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LVRampTime |
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50 [s] |
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LVInjectSpeed |
= |
1.0 [µl/s] |
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; ======================================================================= ; Specify the parameters for the pre- sample and post wash ; procedures: ; ======================================================================= |
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WashMode |
= |
SingleSolvent |
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WashDepth |
= |
86 [%] |
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WasteDepth |
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19 [%] |
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WashSpeed |
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3.0 [µl/s] |
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PreWash |
= |
1 |
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PreWashVolume |
= |
3.0 [µl] |
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PreWashSolvent1 |
= |
A |
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PreWashSolvent2 |
= |
None |
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PreWashSolvent3 |
= |
None |
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PreWashSolvent4 |
= |
None |
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PostWash |
= |
1 |
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PostWashVolume |
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6.0 [µl] |
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PostWashSolvent1 |
= |
B |
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PostWashSolvent2 |
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None |
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PostWashSolvent3 |
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None |
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PostWashSolvent4 |
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None |
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SampleWash |
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1 |
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SampleWashVolume |
= |
4.0 [µl] |
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; ======================================================================= ; Tray temperature parameters ; When using temperature controlled trays, specify temperature parameters as exemplified ; below for each tray used. ; Note: Do not specify these parameters when controlling the Virtual part of ; the sampler. ; ======================================================================= |
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PrimaryTrayTemperature.Ctrl |
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On |
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PrimaryTrayTemperature.Nominal |
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30 [°C] |
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PrimaryTrayTemperature.StandbyCtrl |
= |
On |
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PrimaryTrayTemperature.StandbyNominal |
= |
25 [°C] |
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; ======================================================================= ; GC parameters ; ======================================================================= ; ; ... ; ======================================================================= ; Inject Synchronization and GC run ; Add appropriate GC ramp programs (unless not already included by PGM wizard) ; ======================================================================= |
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0.000 |
Wait Sampler.Ready and GC.Ready |
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Inject |
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GC_1.AcqOn |
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; ... |
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15.0 |
GC_1.AcqOff |
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End |
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For more information on setting up sequences for the TriPlus sampler, refer to
Thermo Scientific TriPlus Sampler/ThermoQuest HS850/HS2000 Headspace Samplers: Tips for Sequences
For more information on controlling the sampler, also refer to:
Thermo Scientific TriPlus Sampler: General Commands
Thermo Scientific TriPlus Sampler: TriPlus AS Commands
The Administrator Help section provides installation details; refer to Hardware Installation:
For information about how to install the sampler, refer to 
Thermo Scientific TriPlus Sampler: Installation.
If you want to operate the sampler with two GCs, refer to Thermo Scientific TriPlus Sampler: Dual Operation.