Commands and Tips for Thermo Scientific Devices
ThermoQuest AS2000/Fisons AS800 Autosamplers

Chromeleon supports the following special commands and parameters for these autosamplers, which are identical in construction (please note that the display Filter level determines which commands and properties are displayed). Also, refer to the information in the manual shipped with the instrument.

 Tip:

Connect the autosampler in Chromeleon only after the hardware has been initialized completely after power up of the autosampler. If hardware initialization is not yet complete, communication errors or initialization errors may occur during the connect.

 

Command

Description

Clean

Cleans the syringe using the previously defined parameters (see below).

Inject

Injects the sample using the previously defined parameters (see below).

InjectSync

Sets the injection mode for inject sychronization:

Normal: (Default setting) Performs a standard injection, i.e., without performing an injection on the second column at the same time.

Twin: Allows simultaneous injections from two autosamplers that are installed on different timebases.

 Tip: 

This function requires that the AS2000 GC Sampler driver is installed.

StopInject

Aborts a running injection.

 Tip:  

Use with care! Might cause incorrect behavior!

 

Use the following parameters to specify the Inject command in detail. Define all injection parameters before initiating the Inject command. Otherwise, they will not take effect.

 

Parameter

Description

AirVolume

Select this parameter to specify the volume of air (0.0 - 500.0 µl) drawn after pulling the syringe needle out of the vial. This reduces evaporation from the syringe needle. The total of AirVolume plus FillingVolume must not exceed the syringe volume.

CleanCycles

Select this parameter to determine the number of cleaning cycles before injection (0...15). Specify the volume with the FillingVolume parameter.

Cleaning Cycles before Injection (Pre Inj Clean Cyc) [Solvent A/B/C/D]

Select this parameter to set the number of pre-injection cleaning cycles with solvent A/B/C/D. Please note: Only the solvent that has been specified last is used.

Cleaning Cycles after Injection (Post Inj Clean Cyc) [Solvent A/B/C/D]

Select this parameter to set the number of post-injection cleaning cycles with solvent A/B/C/D. Please note: Only the solvent that has been specified last is used.

Delay (PullUpDelayTime)

Select this parameter to determine the delay following the up and down movements of the syringe plunger (0.0 - 15.0 s).

Delay after (Post Inj Delay Time)

Select this parameter to determine for how long the syringe remains in the injector after the injection (0 - 63 s).

Delay before (Pre Inj Delay Time)

Select this parameter to determine the delay the syringe remains in the injector before the injection (0 - 63 s).

FillingVolume

Select this parameter to set the sample volume to be used for cleaning and eliminating gas bubbles (see CleanCycles and PullupCount). If the FillingVolume (0.0 - 500.0 µl) is less than the sample volume (Volume), the sample volume will be used instead.

InjectionSpeed

Select this parameter to determine how fast the sample is injected (1 - 100 µl/s).

Post Inj Clean Cyc A/B/C/D

Select this parameter to set post-injection cleaning cycles with solvent A/B/C/D. Please note: Only the solvent that has been specified last is used

PullupSpeed
(AspirationSpeed)

Select this parameter to determine how fast the sample is drawn (1 - 100 µl/s).

Pumps (PullupCount)

Select this parameter to determine how often the syringe plunger is moved up and down before the sample is actually drawn up (0...15). This eliminates bubbles and thus, enhances reproducibility.

Volume (Pre Inj Clean Vol)

Select this parameter to set the pre-injection cleaning volume (0,0 - 500,0 µl).

With Sample (Position)

Select this parameter to specify the sample that shall be used for cleaning (positions 0...15).

The following parameters appear only for the AS2000. Use these parameters to specify the Inject command in detail:

Parameter

Description

AirAspirationMode

If the selected InjectMethod is IntStd, set this parameter to specify the sequence used to draw air, sample, and standard into the syringe (possible values: Post=0 (PST*); Double=1(DBL*)).

InjectMethod

Set this parameter to specify the injection method. Normal=0 (Method A*), IntStd=1 (Method B*).

For more information about the parameters for the Internal Standard injection method, refer to the AS2000 manual.

Injector

Set this parameter to specify the GC inlet into which the standard sample is injected. (possible values: Right=0, Left=1) Note: The AS 2000 Both mode is currently not supported.

InternalStdVol

If the selected InjectMethod is IntStd, set this parameter to specify the volume of the internal standard sample (0-500µl).

IntStdVial

If the selected InjectMethod is IntStd, set this parameter to specify the vial containing the internal standard sample.

RelAirVol

If the selected InjectMethod is IntStd, set this parameter to specify the air volume that is drawn between the internal standard and the sample (0-500µl).

*ThermoQuest nomenclature; refer to the AS2000 manual for details.

For more information, refer to:

 ThermoQuest AS2000/Fisons AS800 Autosamplers: Program Example

 ThermoQuest AS2000/Fisons AS800 Autosamplers: Troubleshooting

The Administrator Help section provides installation details; refer to Hardware Installation:

 Fisons AS800 GC Autosampler

 ThermoQuest AS2000 GC Autosamplers