Commands and Tips for Thermo Scientific Devices
ThermoQuest AS2000/Fisons AS800: Troubleshooting

 Note:

If an error occurs during operation, for example, excessive sample volume, the error is displayed on the instrument only. To continue the analysis, confirm the error message directly on the autosampler by pressing the <Enter>. This is possible after the autosampler keycard has been unlocked in Chromeleon. Use either the Disconnect command or the KeycardUnlocked command. Better yet: Disconnect the autosampler, turn it off and on again, wait until the autosampler is ready, and then reconnect the unit (see Connect).

 

Error

Description

Remedial Action

Missing vial

The following messages appears on the instrument's display:

End Sample

Total Injections 0

In addition, Chromeleon waits forever for an inject response.

Abort the batch.

Bad vial

After some retries, a message like this appears on the instrument's display:

PLG NDL INJ TUR CNTR

--- BSY --- --- *

This happens quite easily if the vials are not perfectly crimped.

Disconnect the autosampler and press the EXIT key (on the autosampler's keycard). When prompted whether to pause, continue, or abort the sample, select Abort. Abort the batch and reconnect.

Illegal parameter (e.g., Volume/
Position > Max or Air + Sample Volume > Max)

An error message appears on the autosampler's display.

Disconnect the autosampler. Press the autosampler key, as necessary.

Continuous beep and display cleared

Severe damage may occur (destroyed syringe, vials etc.)!

Turn off the autosampler immediately!

Initialization error

Error occurred during the initialization of the autosampler or the initialization was incomplete. This error may occur when the autosampler is connected in Chromeleon while hardware initialization is still running.

Disconnect the autosampler in Chromeleon. Turn the autosampler off and on again. Wait until initialization is complete and then re-connect the autosampler in Chromeleon.

Connect error

The autosampler issues a message that the connection failed.

This error may occur when the autosampler is connected in Chromeleon while hardware initialization is still running.

Disconnect the autosampler in Chromeleon. Turn the autosampler off and on again. Wait until initialization is complete and then re-connect the autosampler in Chromeleon.

Strange behavior of any other kind

 

Disconnect the autosampler, turn it off and on again, wait until it is ready, and reconnect.

 

For the commands supported for these instruments and for additional tips, refer to:

 ThermoQuest AS2000/Fisons AS800 Autosamplers

 ThermoQuest AS2000/Fisons AS800: Program Example