Command/Property

Description

AlarmBuzzer

When set to On, the buzzer will signal error conditions.

 Tip:  

If the buzzer signals an error condition (AlarmBuzzer=On), you have to turn the autosampler off and on again to reset the instrument and continue operation. Therefore, avoid using this command when controlling the autosampler under Chromeleon.

CoolingOnTime

Indicates cooling time (in total) of the autosampler (read-only).

Dispense

Dispenses a specified volume. The following options are available:

To: Sample position to which to dispense the volume.

Volume: Volume to dispense (0.0 to 25.0 µl).

SyringeSpeed: Speed used to dispense the volume (1 to 9 - optional setting: if the SyringeSpeed is not set, the PrepSpeed will be used instead (see below)).

SampleHeight: Height at which the sample is dispensed (0 to 40 mm - see below).

Draw

Draws a specified volume. The following options are available:

From: Sample position from which to draw the volume.

Volume: Volume to dispense (0.0 to 25.0 µl).

SyringeSpeed: Speed used to draw the volume (1 to 9 - optional setting: if the SyringeSpeed is not set, the PrepSpeed will be used instead (see below).)

SampleHeight: Height at which the sample is drawn (0 to 40 mm - see below).

FirstTransportVial

Defines the vials to be used for µlPickUp injections. Used together with LastTransportVial.

FlushVolume

Flush volume used for FullLoop and PartialLoop injections (0.1 to 25.0 µl).

Inject

Injects the sample. If you have created a user-defined FAMOS program, e.g., in the  Program Wizard, use the Inject command to start the program. In this case, injection is performed after the InjectMarker command of the user-defined program.

 Note:  

Injection can be performed only if the autosampler is in Not running state. This is usually ensured by the WaitSampler.Ready command that is generated by the PGM Wizard. If the system detects a different state, the following message appears in the Audit Trail: Abort] hh:mm:ss {Sampler} Sampler status must be "Not running" before inject."

InjectionCounter

Total number of injections (read-only).

InjectMode

Injection mode (for more information, refer to the operating instructions for the instrument):

UserProg The autosampler executes the program or sample preparation steps defined by the user.

Partial       The syringe volume is injected in part only (at most 50% of the sample loop volume).

FullLoop   The syringe volume is injected completely.

µlPickUp   The syringe volume is injected completely. However, it contains partly sample volume only. Transport liquid is drawn first, then sample, and then transport liquid again, i.e. the syringe, the sample needle, and both ends of the sample loop are filled with transport liquid. That is why the TransportVial should contain solvent. The sample volume is in the middle of the sample loop.

InjectValve

Injection valve can be in the following positions:
Inject or Load.

ISS_A / ISS_B_Immediate

Switches the associated valve immediately.

LastTransportVial

Defines the vials to be used for µlPickUp injections. Used together with FirstTransportVial.

LoopVolume

Volume of the installed loop (read-only).

LowDispersionFactor

(In Micro mode only) For more information, refer to the FAMOS User's Manual.

LowDispersionFlow

(In Micro mode only) For more information, refer to the FAMOS User's Manual.

LowDispersionMode

Enable low dispersion mode (in Micro mode only).

OperationalMode

Operational mode (Micro or Conventional - also, refer to Hardware Installation  UltiMate Capillay/Nano HPLC System: Installation). Affects some limits, changing resets many operational parameters (read-only).

Output

Sets the corresponding output to the specified state.

PowerOnTime

Indicates the time (in total) the autosampler was turned on (read-only).

PrepSpeed

Speed used as the default syringe speed.

PrepVial

Sample preparation vial for the Draw and Dispense actions.

ReagentXVial
(where X = A to D)

Position of the vial that contains the reagent X or the transportation fluid when UserProg is selected as Inject Mode.

RinsebetweenReinjections

If this parameter is set to No, the rinse step is omitted.

 Note: 

This command allows you to make several injections from the same vial one after the other without loosing sample between the injections by rinsing.

RunScreen

If the command is set to On, the action currently performed by the autosampler is displayed on the instrument.

SampleHeight

The height at which the sample is drawn or dispensed.

Status

Status (read-only).

Syringe

Volume of the installed syringe (read-only).

SyringeCounter

Total number of syringe movements (read-only).

SyringeSpeed

Syringe speed used for draw and dispense operations (Low, Normal, or High). The flow depends on the volume of the installed syringe. (For more information, refer to the FAMOS User's Manual.)

 Note: 

The flow into or from the syringe is the product of the speed defined by SyringeSpeed and the SyringeSpeedFactor.

Example: SyringeSpeed=Low corresponds to 31.3µl/min for the 25µl syringe), SyringeSpeedFactor=0.5. The resulting flow is 15.65µl/min.

SyringeSpeedFactor

Factor used for scaling the syringe speed (see SyringeSpeed).

SyringeValve

Can be in the following positions:
Needle, Wash, or Waste

SyringeValveCounter

Counts the valve movements.

TempCtrl
(with installed Cooling Option only)

Temperature control of the sample rack. The following settings are available: On (cooling/heating enabled) or Off (cooling/heating disabled).

Temperature
(with installed Cooling Option only)

You can indicate the nominal temperature for the tray and thus for the sample. The temperature can be between 4°C to 40°C (39.2°F to 104°F). If you open the command tree, the following commands are available: Value (= current tray temperature, read-only), Nominal (= nominal tray temperature), UpperLimit, and LowerLimit. If the current temperature exceeds the limits, the system aborts the batch and starts emergency handling.

TubingVolume

Volume of the installed needle capillary (read-only).

UseAirSegment

When set to On, an additional air segment is added when loading the sample loop. This reduces sample contamination. (For more information, refer to the FAMOS User's Manual.)

UseHeadSpace

When set to On, additional pressure is applied to the vial while the sample is transported to the loop. This increases the reproducibility but requires airtight vials and/or well septa. (For more information, refer to the FAMOS User's Manual.)

Wash

Starts the wash cycle.

WashVolume

Volume used for the wash cycle.