Dionex Autosamplers
Dionex/LC Packings FAMOS Autosampler: Sample Preparation

The Dionex/LC Packings FAMOS Autosampler provides many sample preparation commands that are used to further define the individual sample preparation steps. The sample preparation commands are accessible from the Advanced level only. Use the  Program Wizard to create a user-defined sample preparation program. This program is started by the Inject command.

Chromeleon supports the following sample preparation commands (please note that the display Filter level determines which commands and properties are displayed):

 

Command/Property

Description

CodeOutput

Communicates a code number (between 0 and 15) to the P2 output (VialNo&Marker (TTL)). For more information, refer to the FAMOS User's Manual.

Compressor

Serves to build up pressure (On) or relieve pressure (Off)

Dispense

Dispenses a specified volume. The following options are available:

To: Sample position to which to dispense the volume.

Volume: Volume to dispense (0.0 to 25.0 µl).

SyringeSpeed: Speed used to dispense the volume (Either select an option (Low, Normal, or High) from the drop-down list or enter the any number between 1 and 9).

SampleHeight: Height at which the sample is dispensed (0 to 40 mm).

Draw

Draws a specified volume. The following options are available:

From: Sample position from which to draw the volume.

Volume: Volume to dispense (0.0 to 25.0 µl).

SyringeSpeed: Speed used to draw the volume (Either select an option (Low, Normal, or High) from the drop-down list or enter the any number between 1 and 9).

SampleHeight: Height at which the sample is drawn (0 to 40 mm).

InjectMarker

Required to start injection.

InjectValve

Injection valve. The valve can be either in Inject or in Load position.

LabeledVialMarker

Creates another marker pulse for different vials, e.g., standard samples.

MixWait

Specify the time the system waits between steps
(0s (0:00:00h) to 35.999s (9:59:59h))

MixNeedleWash

Volume used for washing the needle (10 to 9.999µl).

MoveSyringe

Determines the volume and the speed for loading and dispensing the syringe. For this command, the Load and Unload parameters are available.

 Tip:

Do not use both parameters in the same line of the PGM File because in this case, the PGM File does not pass the Ready Check.

MoveSyringeHome

Returns the syringe to its home position.

Output

Sets the corresponding output to the selected state.

SSV

Solvent Selection Valve. Specify the Position. The following options are available: Solvent A-D.

SyringeValve

Specify the Position. The following options are available: Needle, Wash, or Waste.

Valve_A

Specify the Position. The following options are available:
1_2 (concentrating the sample) or
10_1 (eluting the sample concentrated on the pre-column to the analytical column).

Valve_B

Specify the Position. The following options are available:
10_1 (concentrating the sample) or
1_2 (eluting the sample concentrated on the pre-column to the analytical column).

VialMarker

Generates a marker pulse for sample vials, e.g., unknown samples.

WaitInput

Defines the incoming signal for which to wait.

Wash

Starts a wash cycle.

 

For a basic description of Dionex autosamplers, including the standard commands, refer to  Dionex Autosamplers.

For information about the general commands for the FAMOS autosampler, refer to  Dionex/LC Packings FAMOS Autosampler. (The general FAMOS autosampler commands partly overlap the sample preparation commands listed above.)

For information about how to use the commands in practical operation, refer to Practical Tips for Device Control  Examples for User-Defined Programs for the FAMOS Autosampler (Dionex/LC Packings).