Inject Mode

Select the injection mode. The injection mode selected in the Server Configuration program (see Options (AS-AP Autosampler Dialog Box)) determines which injection mode options are available.

The AS-AP can perform the following types of injections:

• UserProg: Allows the creation of user-defined injection steps. When you select this option, no recommended values are displayed and the Accept recommended values button is disabled. Click Next > to define the program on the   Sampler Options (User-Defined Program) page.

• PullFull: Full-loop injection to one system, using the pull mode

• PullPartial: Partial-loop injection to one system, using the pull mode

• PushFull: Full-loop injection to one system, using the push mode

• PushPartial: Partial-loop injection to one system, using the push mode

• PushPartial_ls: Partial-loop, limited-sample injection (no extra sample is drawn) to one system, using the push mode

• PushConcentrate: Concentrate injection to one system, using the push mode

• PushCap: Injection to a capillary system, using the push mode. When you select the PushCap injection mode, a command specifying a SmallAirBubbleVolume of 20 µl is inserted into the program. The air bubble is used to isolate sample in the tubing when the syringe volume is 1 ml or less.

• PushSimultaneous: Full-loop injections to two systems simultaneously, using the push mode

• PushSeqFull:Full-loop injections to two systems alternately, using the push mode

• PushSeqPartial: Partial-loop injections to two systems alternately, using the push mode

• PushSeqPartial_ls: Partial-loop, limited-sample injections (no extra sample is drawn) to two systems alternately, using the push mode

• PushSeqConcentrate: Concentrate injections to two systems alternately, using the push mode

• PushSeqCap: Injections to two capillary systems alternately, using the push mode. When you select the PushSeqCap injection mode, a command specifying a SmallAirBubbleVolume of 20 µl is inserted into the program. The air bubble is used to isolate sample in the tubing when the syringe volume is 1 ml or less.

To perform sequential injections, the AS-AP must be equipped with the sequential injection option. For more information about injection modes, refer to the autosampler operator's manual.

Flush Volume

(For Pull mode only) Enter the flush volume to be used for the first FullLoop or PartialLoop injection.

 Tip:  

The recommended flush volume is 2 x (Needle Volume + Sample Transfer Line Volume).

Flush Volume 2

(For Pull mode only) Enter the flush volume to be used for additional injections from the same vial.

 Tip:  

Flush Volume 2 can be less than the flush volume for the first injection (assuming no wash cycle between injections). A volume equal to or greater than the needle volume is recommended.

Loop Overfill

The product of the volume of the sample loop and the Loop Overfill factor determines the volume that is drawn in and/or transported through the sample loop for loop flushing before the sample is injected.

The amount of sample used depends on which injection mode you select:

PullFull mode: (Loop Volume x Loop Overfill factor) + 30 µl

PushFull or PushSequentialFull mode: (Loop Volume x Loop Overfill factor) + Flush Volume

Capillary Overfill

The equivalent of the Loop Overfill factor, but for capillary full-loop injections.

Partial Cut Volume

Specify a volume of sample to be discarded from each end of the sample drawn.

Diverter Valve Position

Set the diverter valve to Position 1 (the default) or Position 2.

These options are available only when the sequential injection option is installed.

Cycle Time

Set a uniform time between injections by specifying a cycle time greater than 0 minutes. The time between injections is never shorter than the specified cycle time. However, if the previous program is still running when the cycle is over, the cycle time setting will be ignored.

Note: If the cycle time is set to 0, the time between injections is determined by the commands in the program specified for each sample injection in a Sequence.

Sample Prep Overlap

Select the check box to enable or disable overlap of samples. When Sample Prep Overlap is enabled, the AS-AP starts the next sample in a sequence while data acquisition is occurring for the current sample. Overlapped tasks include all sample preparation functions (flushing the injection port, dilution, pipetting from one vial to another, mixing the vial, etc.).

When the AS-AP is in concentrate mode, the concentrate, reagent prime, and reagent flush functions can be overlapped. Sample injection does not occur until the current sample is finished.

Sample Prep Overlap is disabled when the AS-AP is shared by two timebases. When Sample Prep Overlap is disabled, the autosampler always waits until data acquisition is completed for a sample before starting the next sample.

For more information, refer to Practical Tips for Device Control: Autosampler Control Overlapping Samples.

Temperature Control

Select the check box to define temperature control options.

This option is available only when the sample temperature control option is installed.

Temperature

Enter a temperature set point for the sample temperature control option, which provides heating and cooling of the sample tray.

This option is available only when the Temperature Control check box is selected.

Deviation

Enter the maximum deviation in the temperature set point for the sample temperature control option.

This option is available only when the Temperature Control check box is selected.

Wait for Temperature

Select the check box to delay the start of the program until the temperature of the sample tray has stabilized.

This option is available only when the Temperature Control check box is selected.

Reagent Flush

Select the check box to enable a reagent flush. In this operation, reagent is transferred from a vial to the injection port and flushed onto the concentrator column installed on the injection valve (after the sample has been delivered to the concentrator column).

This option is available only when the selected Inject Mode is PushConcentrate or PushSeqConcentrate.

Source Vial

The reservoir from which to pick up reagent when Reagent Flush is selected.

Source Volume

The volume of reagent to be pipetted when Reagent Flush is selected.