Calibration Point, Calibration Level, Replicates

If the peak area is determined for the known amount of a standard sample, the point resulting from both values in an x, y-diagram is referred to as the calibration point.

If the corresponding peak area is determined several times for the same Amount, different area values are received, depending on the precision. Thus, a larger number of calibration points is obtained. Calibration points based on the same concentration are referred to as replicates of the same concentration level.

Calibration points of different levels are obtained when area values are determined for various amounts of a standard. This can be achieved by a Dilution Series or by varying the injection volume.

Based on the calibration points, the data system calculates the calibration coefficients of the Calibration Function selected by the user.

For more information, refer to:

Theory of Calibration  Calibration (Overview) and  Calibration Principle

Calibrating  Weighting and Averaging Calibration Points