Calibration (Overview)

If the signal of a chromatography detector is proportional to the concentration of a substance in the flow cell, it is suitable for quantitative determination. This is a characteristic, for example, of the absorption supplied by a UV detector in the scope of the Lambert-Beer law.

The proportionality constant depends on the chemical quality of the substance of interest and on the physical properties of the used detector. For UV detectors, these are mainly the optical wavelength and the spectral bandwidth. As integration programs can determine only the area (and height, respectively) below a peak, conversion into absolute amount or concentration units is possible only if calibration was executed before the analysis.

For more information, refer to:

 Calibration Principle

 Calibration Types (Linear)

 Calibration Types (Non-linear)

 Using the Calibration Curve

 Calculating the Calibration Curve

 Standard Methods

 Evaluation with Various Standard Methods

 Evaluation with the Standard Addition Method

 Implementation

Using the available calibration points, Chromeleon creates calibration curves for each calibrated substance at the end of the calibration. The curves are represented and evaluated in the calibration curve method window (see Data Representation and Reprocessing  The Calibration Curve).