Type:

Floating point value

Dimension:

ml

Value Range:

system-dependent

Default:

20.0

Related Parameters:

Dil. Factor (dilution factor)

Weight (Sample Weight Factor)

Description:

The Sample Variable Injection Volume defines the injection volume in micro liters (µl). In automatic operation, the installed Driver converts this value into a volume readable by the autosampler, then the value is sent to the Autosampler.

You can enter different injection volumes to create a Dilution Series for multiple-point calibration (Single-Point and Multiple-Point Calibration).

Function:

In multiple-point calibration with differing injection volumes, the concentration of the second calibration sample is calculated from the injection volumes of the first and the second sample. The same principle (doubling injection volume equals doubling the amounts of each component) is applied to all subsequent calibration samples.

In a multi-point calibration with a "concentration series" (varying injection volume) which was created with an automatic autosampler it is therefore only necessary to enter the component amounts for the first standard sample into the peak table. Chromeleon calculates all additional calibration values, that is, the corresponding amounts of the subsequent standard samples (Type: Standard).

 Note:

In order to minimize carry-over effects in such a multiple-point calibration, standard order should always be from lowest to highest concentration.