Single-Point and Multiple-Point Calibration

If the position of the calibration curve is determined only by the Calibration Points of one Calibration Level, this is called single-point calibration. In this case, the Calibration Type linear must be chosen.

If calibration points on different levels determine the position of the calibration curve, this is referred to as multiple-point calibration; for example, two-point, three-point, etc. calibration. The number of calibration levels determines possible calibration types.

In both cases, the number of replicates is irrelevant for the number of calibration levels. Replicates only serve to secure the measuring results statistically.