Defining Sample Preparation Steps (AS-AP)

Autosampler Control
Defining Sample Preparation Steps (AS-AP)

Sample preparation steps for the AS-AP Autosampler are part of a Program and can be defined using the Program Wizard (see Control The Program Wizard).

Perform the following functions from the Sampler Options page in the Program Wizard.

Defining a New Sample Preparation Step

Select a Procedure from the list, and then select the parameter values for the function (see the table below).
Click Insert Above to add the step to the list, above the currently selected step (if any). Click Insert Below to add the step to the list below the currently selected step (if any).

Changing an Existing Step

Select the step in the list.
Make the required changes; for example, select a new procedure and enter its parameters, or change a parameter for the current procedure.
Click Change.

Tip:

To delete a step, select it, and click Delete.

Function	Description	Parameters
Pipet	Move sample between vials	Source: the vial from which to pick up the sample volume Source Volume: the amount (µl) of sample to be pipetted Destination Vial: the vial in which to add the sample volume

Mix	Mix vial contents by shaking the tray	Number of times to shake: the number of times to shake the tray before sample is drawn Select the Mix command when using the Pipet command and 10 ml vials. It is not necessary to manually add a mix command to the sample preparation table when using the Dilute or DiluteWithWash command (see below); the AS-AP automatically mixes vials after executing the Dilute and DiluteWithWash commands.

Dilute	Dilute sample with reagent from a vial	Source: the vial from which to pick up the sample volume Source Volume: the amount of concentrated sample to be diluted Destination Vial: the vial in which to add the concentrate Diluent: the vial that contains the diluent Reagent Volume: the amount (µl) of reagent to be used When the Dilute command is executed, the AS-AP automatically performs a mix operation; thus, it is not necessary to manually add a mix command to the sample preparation table. The default dilution mixing method depends on the vial size: For 0.3 ml and 1.5 ml vials, the autosampler uses a tray shake operation. For 10 ml vials, the autosampler uses a draw-and-dispense operation.

DiluteWithWash	Dilute sample with wash liquid	Source: the vial from which to pick up the sample volume Source Volume: the amount of concentrated sample to be diluted Destination Vial: the vial in which to add the concentrate Reagent Volume: the amount (µl) of wash liquid to be used When the DiluteWithWash command is executed, the AS-AP automatically performs a mix operation; thus, it is not necessary to manually add a mix command to the sample preparation table. The default dilution mixing method depends on the vial size: For 0.3 ml and 1.5 ml vials, the autosampler uses a tray shake operation. For 10 ml vials, the autosampler uses a draw-and-dispense operation.

Mix_DrawDispense	Mix vial contents by repeatedly drawing and dispensing a volume of the vial contents	Source: the vial from which to pick up the sample volume Source Volume: the amount of vial contents to draw and dispense; the volume must be the lesser of these two values: 70% of the volume in the vial or 70% of the buffer loop volume Select the Mix_DrawDispense command when using the Pipet command and 0.3 ml or 1.5 ml vials. It is not necessary to manually add a mix command to the sample preparation table when using the Dilute or DiluteWithWash command (see above); the AS-AP automatically mixes vials after executing the Dilute and DiluteWithWash commands.

Concentrate and Sequential Concentrate Function The following function is designed to be used when the AS-AP is in Concentrate or Sequential Concentrate mode.
ReagentFlush	Transfer reagent from a vial to the injection port; typically used to flush reagent onto a concentrator column installed on the injection valve before loading sample	Source: the reservoir from which to pick up reagent Volume: the amount of reagent to flush through the concentrator The ReagentFlush command is performed before sample is drawn and is delivered before the injection procedure.

Also, refer to:

Defining Sample Positions (AS-AP)

Overlapping Samples