Thermo Scientific TriPlus Sampler: Creating a Program for Headspace AnalysisCommands and Tips for Thermo Scientific Devices
Thermo Scientific TriPlus Sampler: Creating a Program for Headspace Analysis
Follow the steps below to create a program for the TriPlus headspace sampler:
Use the PGM Wizard to create a program for the timebase.
Add all relevant settings for the headspace sampler in the PGM Editor. Use the example program below for assistance.
The settings must appear before the first time step, i.e., before t = 0.000 min.
Add a Wait Sampler.Ready AND GC.Ready statement directly before the Inject command unless not already included by the PGM wizard.
Tips:
The GCCycleTime should exceed the total of the GC's program run time and equilibration time. Note that the GC needs some time after a run to become ready for the next injection (to cool down and equilibrate).
Example Program for Single Mode
The following program is a typical example of a headspace sequence for a TriPlus HS sampler in Single mode:
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;======================================================================= ; Injection Customization: General ;======================================================================= |
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InjectMode |
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Single |
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InjectionsPerVial |
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1 |
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GCStartMode |
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Anticipated |
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LoadsPerInject |
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1 |
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; LoadRepetitionTime, only required if LoadsPerInject > 1 |
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LoadRepetitionTime |
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0.5 [min] |
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; ======================================================================= ; Temperature Settings ; To deactivate a temperature control, set the respective '*.Ctrl' ; property to 'Off' and ; comment out the associated '*.Nominal' parameter (see below). ; ======================================================================= |
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OvenTemperature.Ctrl |
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On |
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OvenTemperature.Nominal |
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40 [°C] |
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OvenTemperature.StandbyCtrl |
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On |
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OvenTemperature.StandbyNominal |
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45 [°C]] |
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NeedleTemperature.Ctrl |
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On |
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NeedleTemperature.Nominal |
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50 [°C] |
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NeedleTemperature.StandbyCtrl |
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Off |
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;NeedleTemperature.StandbyNominal |
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40 [°C] |
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; ======================================================================= ; Time Program ; ======================================================================= |
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IncubationTime |
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10.5 [min] |
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The GCCycleTime must be significantly longer than the PGM run time. |
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Note that the GC needs some time |
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after a run to become ready for the next injection (to cool down and equilibrate). |
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The exact time depends on the GC program (and the GC type). |
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Ensure that GCCycleTime is sufficient enough for the GC to become 'Ready to Inject' |
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before the preparation time elapses. |
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GCCycleTime |
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12.5 [min] |
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ShakerOnTime |
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30 [s] |
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ShakerOffTime |
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30 [s] |
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Setting of VentingTime is only required when using MHE mode |
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VentingTime |
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10 [s] |
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; ; |
Setting of AnticipatedColdTrapStart is only required when using enrichment or having a cooled inlet |
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AnticipatedColdTrapStart |
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3.0 [min] |
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; ======================================================================= ;Sampling Parameters ; ======================================================================= |
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SampleDepth |
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Top |
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PreFill |
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On |
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FillStrokes |
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2 |
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FillVolume |
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2.0 [ml] |
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PullupDelay |
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4 [s] |
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DrawSpeed |
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20 [ml/min] |
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DownSpeed |
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30 [mm/s] |
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; ======================================================================= ;Sample Injection Parameters ; ======================================================================= |
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Injector |
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InjectorA |
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InjectSpeed |
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30 [ml/min] |
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PreInjDelayTime |
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10 [s]] |
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PostInjDelayTime |
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10 [s] |
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PreInjSyringeFlush |
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Off |
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PostInjFlushTime |
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30 [s] |
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InjectDepth |
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30 [mm] |
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StandbySyringeFlush |
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Off |
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; ======================================================================= ; Wash Parameters (only available if wash station is installed) ; ======================================================================= |
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WashMode |
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SingleSolvent |
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PostWash |
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1 |
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LVInitialInjectTime |
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2.0 [ml] |
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PostWashSolvent1 |
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A |
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PostWashSolvent2 |
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None |
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PostWashSolvent3 |
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None |
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PostWashSolvent4 |
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None |
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DryTime |
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30 |
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WashFrequency |
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After5Samples |
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; ======================================================================= ; Add your GC parameters below ... ; ======================================================================= |
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; ... |
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; ======================================================================= ; Inject Procedure and GC Time Program (to be added if not included by the PGM wizard) ; ======================================================================= |
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0.000 |
Wait GC.Ready and Sampler.Ready |
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Inject |
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GC_1.AcqOn |
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; ... |
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10.0 |
GC_1.AcqOff |
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10.1 |
End |
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Example Program for Progressive mode
The program for a headspace sampler in Progressive mode is similar to the above program except for the injection mode and time program settings. The following program lines show the relevant settings for a PGM file in which the first sample of the sequence incubated for 4 minutes, the second sample for 6 minutes, the third for 8 minutes, etc.:
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;======================================================================= ; Injection Customization: General ;======================================================================= |
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InjectMode |
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Progressive |
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... |
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; ======================================================================= ; Time Program ; ======================================================================= |
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IncubationTime |
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4.0 [min] |
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IncubTimeOffset |
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2.0 [min] |
Appending Samples to a Run in Progressive Mode
In Progressive mode, the incubation time of the second and all subsequent samples is calculated by the following formula:
IncubationTime+ (i-1)*IncubTimeOffset
However, the formula only applies to samples within a sequence. If a Chromeleon sequence is split into several headspace runs, or processing of a cancelled or interrupted sequence is resumed, a new Progressive cycle is started in each individual sequence using the initial incubation time (e.g., 4 minutes in the program example above). As this behaviour of the TriPlus sampler may lead to unexpected results, it is recommended to refer to the RealIncubationTime parameter for information about a sample's actual incubation time.
For more information on setting up sequences for the TriPlus sampler, refer to
Thermo Scientific TriPlus Sampler/ThermoQuest HS850/HS2000 Headspace Samplers: Tips for Sequences
For more information on controlling the sampler, also refer to:
Thermo Scientific TriPlus Sampler: General Commands
Thermo Scientific TriPlus Sampler: TriPlus HS Commands
The Administrator Help section provides installation details; refer to Hardware Installation:
For information about how to install the sampler, refer to 
Thermo Scientific TriPlus Sampler: Installation.