Sample Definition

In Chromeleon, the term sample is defined as follows: Each injection is an individual sample. Thus, multiple injections from the same sample vial under similar conditions are several samples.

Sample definition is the process of determining how much of a substance is injected, from which vial and under which conditions it is injected, and which evaluation parameters are used. Distinguish between a single sample and a series of samples (= sequence).

Single Sample

To enter a single sample, you can perform all required information and user commands via the keyboard or mouse.

For manual injections, click the Inject icon on the Online toolbar, enter the injection volume, and perform the injection via a hand-operated valve. If an Autosampler is available, determine the sample position via the Inject command. Afterward, start data acquisition via the AcqOn/Off command. When the end of the sample is reached, end data acquisition by clicking the Acq Off icon on the Online toolbar.

The recorded data is temporarily saved to the manual sequence of the default Datasource of the system. In network operation, this datasource is identified by the name of the computer from which the user logged on to the  Network.

When data acquisition is complete, Chromeleon prompts you to enter the final storage location for the temporarily saved data.

Sequence/Batch

If several samples are to be processed successively, they are included in a sample list (sequence), together with the instrument control and evaluation information.

The samples are processed automatically. (For more information, refer to Batch.)

For more information about how to create a sample list, refer to  The Sample List (Sequence).