Installing and Controlling Third-Party Devices
Agilent LC System: Installation

Device Type:

HPLC system

Device Driver:

Agilent: LC System

Device driver for LC systems integrated in the Agilent Instrument Control Framework (ICF)

Supported Hardware Options:

For the most recent information on ICF versions available, supported LC modules and required firmware versions, refer to Agilent's website at www.agilent.com, or click the following link: Specifications – Compatibility Matrix.

If your system configuration does not comply with the modules and minimum firmware requirements suggested by Agilent, your system may not be supported by the ICF. In this case, the Chromeleon LC System driver cannot be used. As an alternative, you can use the 1100 HPLC System or 1200 HPLC System driver.

PC Requirements:

For information about PC requirements please refer to the Chromeleon Release Notes.

Restrictions:

  • Only one chromatography data software running an ICF application is supported per PC

  • Only one injector is allowed per instrument

  • Only one DAD 3D channel supported per timebase (only the channel of the first DAD if multiple DADs are installed)

  • Recording of 3D data using an FLD is not supported (2D data can be recorded without restrictions)

  • Acquisition of 3D data using a data collection rate of 160 Hz may result in abort of the sample. Either use a larger wavelength range or reduce the spectra step in order to acquire 3D data at a data rate of 160 Hz.

  • Collecting data from runs started via handheld controller not supported

  • Data storage is at variable data rates (Step=Auto) to reduce disc consumption.

    Note: If a fixed data rate is desired, determine the data rate by using the Peak Width – Data Rate table provided in the user manual of the respective detector.
    For each channel add Step=n to the Chromeleon PGM file, whereby n= 1/data collection rate. Example (DAD): At a peak width of 0.1min, the data rate is 2.5 Hz. As a result,  resulting in DAD_Signal_A.Step=0.4.

  • Inject synchronization via software using an external autosampler is not supported.
    Inject synchronization via hardware using a remote cable is supported, for example using the CTC PAL or UltiMate 3000 autosampler (also, see section Hardware Prerequisites below).

  • Use of third-party detectors which are not part of the Agilent instrument is not recommended due to poor retention time reproducibility.

  • Manual injection via Chromeleon not supported

  • The following commands are not supported: Hold, Continue, StopFlow, Wait and Message

  • Emergency and power failure programs not supported

  • Rack layout/preview not supported

  • No support of diagnostics functions. The Agilent Lab Advisor software is required to perform diagnostics operations.
    Note:
     Modules equipped with older LAN cards are not able to communicate with Chromeleon and the Lab Advisor simultaneously.

  • Programs must be created using the Program Wizard in combination with the Agilent instrument method editor. Manual editing in the PGM Editor is restricted to a few commands.

  • Fraction collection is not supported for the Agilent ICF.

 

What is required?

License:

Timebase Class 2
3D Data Acquisition (to allow acquisition of 3D data from Diode Array Detectors).

Connection:

Connection is via LAN. Instrument control via GPIB is not possible.

 

Hardware Prerequisites:

To operate the LC system with an external autosampler (for example, a CTC PAL or an UltiMate 3000 autosampler), the following cables are required:

  • Agilent remote cable 01046-60201 (general purpose cable) available from Agilent

  • 6-pin Mini-DIN signal cable (5m; Dionex part no. 6000.1004) for the relay contact outputs of the Ultimate-3000 autosampler.

For information on connecting the cables, refer to Agilent Remote Cable (LC System).

Software Prerequisites:

  • Windows XP SP3 or Windows Vista (32-bit) is required for installing the ICF.

  • .net Framework (version 3.0 SP1) must be installed.

  • CM 6.80 SR10 or higher

  • The Instrument Control Framework (ICF) software package from Agilent must first be installed on the PC before you can install the driver. The software package is provided on the Chromeleon DVD and can also be obtained free of charge from Agilent.

Tip:

The required  ICF software version for use with Chromeleon is A.02.01 or later (included on the Chromeleon CD).

Firmware Requirements

Firmware Compatibility

To ensure proper operation of the LC system, verify that each module's firmware revision is compatible with

  • the firmware of the ICF

  • the firmware revisions installed in the other LC modules

 

Beginning with revisions A.06.1x and B.06.1x, the following rules apply:

  • All minor revisions within a decade (e.g., A.06.10 to A.06.19 or B.06.10 to B.06.19) are compatible among themselves and may be mixed within a LC stack. The same applies to revisions of decades A.06.2x / B.06.2x, and A.06.3x / B.06.3x.

  • A and B revisions are compatible if they are of the same decade, for example, A.06.10 and B.06.12.

  • All revisions of different decades, for example, A.06.1x versus A.06.2x, or B.06.1x versus B.06.2x, are not compatible with each other and must not be used within the same LC stack.

Control Panel:

The following panel is provided in the Dionex Templates\Panels\LC_Other folder in the Browser: Agilent_ICF.pan

 

Hardware Installation

  1. Make sure that the modules installed in your system and firmware version are compatible for use with the ICF.

  2. Connect the modules via LAN. For details, refer to Agilent LC System/1200 HPLC System: LAN.

  3. After setup of the instrument, follow the steps described under Software Installation below.

 

Software Installation

  1. Install the ICF software package from the Chromeleon DVD (in the Drivers\Agilent Instrument Control Framework folder). The package consists of the ICF setup (ICFInstallPackage.msi) and the driver package setup (LCDriverInstallPackage.msi).

  1. Before you install the LC System device driver it is recommended to test the installation using the "Agilent.InstrumentControl.TestApp.exe" test application provided by Agilent. The test helps you to identify network related issues at an early stage. The application is provided under C:\Program Files\Agilent Technologies\Instrument Control Framework. To run the test:

  1. Install the LC System device driver in the Chromeleon Server Configuration:

 

Unattended Installation

For setup automation, standard msi commands can be used to install the software packages, for example:

 

Hints for Software Updates

This section provides information about what to observe after update of the ICF software package and/or Chromeleon. The following two use cases can be distinguished:

  1. Update of ICF software package and Chromeleon software

    After updating both the ICF software and Chromeleon remove the LC driver from the instrument and then add it again. This is to ensure that all new modules are available in Chromeleon and that signal configuration is correct.

  1. Update of ICF software package only

    When you update the ICF software package without updating Chromeleon, Chromeleon will not be able to automatically configure the new modules supported by the ICF. Therefore, the module names in the Agilent Configuration Editor should be checked and corrected, if necessary. Also, adapt the channel names and signal configuration on the Signals (2D) and Spectra (3D)/Scans configuration tab pages, if needed. Signals that cannot be assigned to Chromeleon channels are indicated by the generic (Gen) signal type.

 

Troubleshooting

Problem: When attempting to install the driver or launch the ICF status window, the following error message may be displayed:

File or assembly name "file:///C:\chromel\bin\DDK\V1\Agilent.InstrumentControl.Interface.dll", or one of its dependencies, was not found. The system cannot find the file specified.

Reason: The ICF software package is not installed correctly or not installed at all.

To remedy the situation, install the ICF software package by following the description above.

 

Problem: The following error message appears during device configuration of the Agilent LC system: "xyz cannot be loaded. Could not find file [directory path\filename]".

Reason: The file "ApplicationCustomization.xml" is missing.

To solve the problem, copy ApplicationCustomization.xml from C:\Chromel\bin\DDK\V1\Drivers\Agilent\ICF or from the Chromeleon DVD (in folder Drivers\Agilent Instrument Control Framework) and paste it into the location indicated in the message.

 

Problem: The Agilent module switches from a Ready state to a NotReady state.

Reason: External influences, such as gas bubbles in the RI detector or a lack of eluent, could result in a NotReady state of an Agilent module. For these instances it might be necessary to fix the NotReady condition manually, for example by purging the module or rebalancing the RI detector and to restart the batch afterwards.

 

Further Information

For more installation details, refer to the respective instrument manual.

For an overview of the different Agilent/HP instruments for which device drivers are available in Chromeleon, refer to  Agilent (formerly HP).