Mix  

 

Instrument Type:

Autosampler

Related Commands:

Inject

Dispense

Description:

The Mix command causes the autosampler to mix defined injection volumes (see Volume) from several sample vials (see Position) in a different sample vial.

Function:

When the action is completed, the autosampler returns the Sampler.Ready signal.

Example (ASI-100 Series):

 

0.000 PrepVial BA1

0.000 PrepVolume 10

0.000 Sampler.PrepVial Position + GA1 - RA1

0.000 PrepSubject PrepVial

0.000 Mix 

 

 

The above command sequence mixes 10 µl from a vial in the green segment with 10 µl of a vial in the red segment in the corresponding vial of the blue segment.

Example (AS/AS50):

 

Sampler.Pipet Volume = 20.0, SourceVial = 1, DestinationVial = 10

Sampler.Mix SourceVial = 10, NumberOfTimes = 5, Volume = 30.0

 

In the above command sequence, 20 µl is pipetted from vial 1 and delivered to vial 10. The contents of vial 10 is mixed by drawing in and expelling 30 µl of the vial contents. The mixing cycle is repeated 5 times.

You can also use the PrepSpeed (ASI-100) or SyringeSpeed (AS/AS50) command to determine the draw speed of the syringe.

 Tip:

 

When using highly viscous liquids or liquids with a low boiling temperature, allow the autosampler more time to draw the exact volume exactly without bubbles.

 

For more information, refer to Practical Tips for Device Control:

 Autosampler Commands (ASI-100 Series)

 Autosampler Commands (AS/AS50)