Autosampler Control
Autosampler Commands (GINA 50)

Suck (see Draw)/Dispense Sample

 Note:

In the case of the Dionex Autosampler GINA 50, the corresponding operations can be synchronized with Chromeleon via a remote input and the Suck and Dispense commands. The device automatically reports the completion of each operation to Chromeleon. In the Program, the wait command Sucked must be inserted. All following commands are executed only after the autosampler confirmed the execution of the Suck command, that is, after the sample has been drawn. The program performs this independently of the time required by the autosampler for the suck and dispenses processes. This is shown in the following example:

Description of the program part: First, the Autosampler GINA 50 draws 30 µl solution from Position 20 and afterward draws 30 µl solution from position 21. The entire drawn volume is dispensed in the current vial (current = last position if no other position is specified). Then, the injection volume, which has been specified in the sample list, is drawn and injected from there.

Short command syntax:

Relative Sample Location

The Dionex Autosampler GINA 50 is capable of executing the suck and the dispense commands relative to a certain sample position. The Position+Location is entered in the field Position of the Suck or Dispense commands. In the program this is expressed as follows:

Description of the program part: The autosampler takes 30 µl of solution from position 20, moves 10 positions from the current position in the sample list, and takes 30 µl of solution from there. Then, the 60-µl solution is dispensed into a vial that is located 20 positions from the current sample vial.

Air Segment

For the Dionex GINA 50 Autosampler, the input position = 100 is an imaginary Air Vial from which a certain air volume can be drawn as a separating segment; for example:

Description of the program part: After 30 µl of solution is sucked from position 20, 30 µl of air is sucked before another 30 µl of solution is sucked from position 21. Thus, the two solutions do not encounter each other.

 Note:

The Segment command must always be used in combination with the Dispense command; otherwise, the sucked air volume will be injected, also.

Dispense in Needle Seat

Entering position 101 in a Dispense command enables dispensing the volume contained in the needle into the needle seat of the Autosampler and thus into the waste container.

Wash Injection Loop

Select the Wash command to rinse the injection loop of the Gina 50 autosampler with solvent. This corresponds to the normal solvent flow during the Inject command.

Lift/Lower Sample Needle

Select the NeedleUp command to raise the sample needle. When raising the needle, an active Wash process is automatically interrupted, i.e., solvent flow is not through the injection loop any longer, but directly from the pump to the column.

Execute the Wash command to lower the needle again and direct solvent flow through the sample loop again (for more information, see Autosampler).

The combination of the two commands prevents crystallization of substances in the sample loop.

For an overview of the individual commands for the GINA 50, refer to Commands for Controlling Dionex Devices  Dionex GINA 50 Autosamplers.

Also, refer to  Autosampler Commands (ASI-100 Series).