Merck Hitachi Autosamplers

Commands and Tips for Third-Party Devices
Merck Hitachi Autosamplers

Supported Autosamplers

For Autosampler…	…controlled via…	…use device driver
AS2000	RS-232	Merck Hitachi AS2000 Autosampler
AS4000	RS-232	Merck Hitachi AS4000 Autosampler
L7250	RS-232	Merck Hitachi L7250 Autosampler
L7250	D-Line	Merck Hitachi L7200/L7250 AS (D-Line)
L7200	D-Line	Merck Hitachi L7200/L7250 AS (D-Line)

Supported Device Properties

In addition to the standard autosampler commands (see Dionex Autosamplers), the Merck Hitachi autosampler support the following commands and properties (please note that the display Filter level determines which commands and properties are displayed):

Property	AS2000	AS4000	L7250	L7200
InjectPortWashs	0-20	0-20	0-20	0-20³
InjectPortWashSpeed	1 (Low) ... 5 (High)	1 (Low) ... 5 (High)	1 (Low) ... 5 (High)	1 (Low) ... 5 (High)3
NeedleWashs	1-20	1-20	1-20	1-20³
NeedleWashSpeed	1 (Low) ... 5 (High)	1 (Low) ... 5 (High)	1 (Low) ... 5 (High)	1 (Low) ... 5 (High)³
WasteVolume	Not supported	Not supported	0-4000.0 µl	0-4000.0 µl²
WashVolume	10.0-100.0 µl	10.0-1000.0 µl	10.0-1000.0 µl
LeadVolume	0-100.0 µl	0-1000.0 µl	0-1000.0 µl	0-500.0 µl²
RearVolume	0-100.0 µl	0-1000.0 µl	0-1000.0 µl	0-500.0 µl²
FeedVolume	0-100.0 µl	0-1000.0 µl	0-1000.0 µl	0-500.0 µl²
DeadVolume	0-100.0 µl	0-1000.0 µl	0-1000.0 µl
Syringe	0-100.0 µl	0-1000.0 µl	0-1000.0 µl
InjectionMethod	0 (Compatibility) 1 (Cut) 2 (All)	0 (Compatibil.) 1 (Cut) 2 (All)	0 (Compatibil.) 1 (Cut) 3 (FullLoop)	1 (Cut) ... 3 (FullLoop)²
SyringeSpeed	1 (Low) ... 5 (High)	1 (Low) ... 5 (High)	1 (Low) ... 5 (High)
NeedleDownSpeed	1 (Slow) ... 2 (Fast)	1 (Slow) ... 2 (Fast)	1 (Slow) ... 2 (Fast)	1 (Slow) ... 2 (Fast)
VolSyringe	1 (100µl) - 2 (500 µl)	1 (100 µl) -5 (5000 µl)	1 (100 µl) -5 (5000 µl)	Not supported
PumpPlungerWash	Not supported	Not supported	0 (No) or 1 (Yes)	0 (No) or 1 (Yes)³
VolOptionalSyringe	Not supported	1 (100 µl) - 5 (5000 µl)	Not supported	Not supported
NumberOfDilutors	Not supported	1 or 2	Not supported	Not supported
Dilutor	Not supported	1 or 2	Not supported	Not supported
RackCode	1 - 16	1 - 16	1 - 16	Not supported
Volume	1 - 500.0 µl	1 - 5000.0 µl	1 - 5000.0 µl	1 - 500.0 µl
Position	1 - 100	1 - 200	1 - 200	1 - 200

² If one of these properties is set, all other settings that are marked with ² are set, too. Check the parameters sent to the autosampler with extra care.

³ If one of these properties is set, all other settings that are marked with ³ are set, too. Check the parameters sent to the autosampler with extra care.

Supported Device Commands

Command	AS2000	AS4000	L7250	L7200
Connect (/Disc.)	Supported	Supported	Supported	Supported
Disconnect	Supported	Supported	Supported	Not supported
Aspirate (see Draw)	0 - 500.0 µl, Speed	Dilutor, 0 - 5000.0 µl, Speed	0 - 5000.0 µl, Speed	Not supported
Dispense	0 - 500.0 µl, Speed	Dilutor, 0-5000.0 µl, Speed	0-5000.0 µl, Speed	Not supported
GotoTube	1 - 100, RackCode	1 - 200, RackCode	1 - 200, RackCode	Not supported
GotoWashPort	Supported	Supported	Supported	Not supported
GotoInjectPort	Supported	Supported	Supported	Not supported
MoveNeedle	X:0-163.0 mm Y:0-68.0 mm Z:0-38.0 mm	X:0-244.0 mm Y:0-163.0 mm Z:0-57.0 mm	X:0-156.0 mm Y:0-156.0 mm Z:0-57.0 mm	Not supported
Height	Z:0-38.0 mm	Z:0-57.0 mm	Z:0-57.0 mm	Not supported
GotoSyringePos	0 - 500.0 µl, Speed	Dilutor, 0-5000.0 µl, Speed	0 - 5000.0 µl, Speed	Not supported
GotoSValvePos	0 (Bottle) or 1 (Needle)	Dilutor, 0 (Bottle) or 1 (Needle)	0 (Bottle) or 1 (Needle)	Not supported
GotoValvePos	0 (Inject) or 1 (Load)	0 (Inject) or 1 (Load)	0 (Inject) or 1 (Load)	Not supported
Home	Supported	Supported	Supported	Not supported
Reset	Supported	Supported	Supported	Supported
Wash	Supported	Supported	Supported	Not supported
WashPumpPlunger	Not supported	Not supported	Supported*	Not supported
Inject	Supported - see below	Supported - see below	Supported - see below	Supported
Wash_X	Supported - see below	Not supported	Supported - see below	Not supported
WashDil_X	Not supported	Supported - see below	Not supported	Not supported
GotoOpValvePos	Not supported	Not supported	0 (Inject) or 1 (Load)*	Not supported

Inject Programs

AS2000 AS4000 L7250	Depending on the current value of the Syringe device property, three different programs are internally executed during processing an Inject command. For AS2000 and L7250 the dilutor property always equals 1.
	Syringe>0	GotoSyringePos SyringePos=0 Speed=<current value of property Speed> Dilutor=<current value of property Dilutor> GotoSValvePos SValvePos=Bottle Dilutor=<current value of property Dilutor> GotoSyringePos SyringePos=<current value of property Syringe> Speed=<current value of property Speed> Dilutor=<current value of property Dilutor> GotoTube TubeNumber=<current value of property Position> RackCode=<current value of property RackCode> Aspirate AspirateVol=<current value of property Volume> Speed=<current value of property Speed> Dilutor=<current value of property Dilutor> GotoInjectPort GotoValvePos ValvePos=Load GotoSyringePos SyringePos=0 Speed=<current value of property Speed> Dilutor=<current value of property Dilutor> GotoValvePos ValvePos=Inject
	Syringe=0	GotoTube TubeNumber=<current value of property Position> RackCode=<current value of property RackCode> Aspirate AspirateVol=<current value of property Volume> Speed=<current value of property Speed> Dilutor=<current value of property Dilutor> GotoInjectPort GotoValvePos ValvePos=Load GotoSyringePos SyringePos=0 Speed=<current value of property Speed> Dilutor=<current value of property Dilutor> GotoValvePos ValvePos=Inject
	Syringe<0	GotoValvePos ValvePos=Inject
L7200	Based on the current parameter settings, the autosampler performs an injection. The Inject command transmits the vial number and the sample volume only.

Wash Programs

AS2000

AS4000

L7250

This is the program that is run when the extended wash command (Wash_X resp. WashDil_X) is executed. For AS2000 and L7250 the dilutor property always equals 1.

GotoWashPort

GotoSValvePos SValvePos=Bottle