Dionex Sample and Fraction Manager
Sample and Fraction Manager: Tips for Injection

Chromeleon supports various parameters for sample loading and injection.

Parameters supported for sample loading:

Position, Volume, AirVolume, FillSpeed, FillStrokes, and PullupDelay

Parameters supported for injection:

PreInjDelay, PostInjDelay, InjSpeed, and Injector.

 

When performing an inject command, the Sample and Fraction Manager performs the following steps:

  1. The sample needle is moved to the specified tube.

  2. The specified sample volume is drawn into the syringe.

  3. Optional: If the AirVolume parameter is set to a value > 0, air is drawn from outside the tube.

     Note:

    Steps 1 through 3 are omitted if they were already performed via the PrepareThisSample or PrepareNextSample commands.

  4. The sample needle is moved to the specified injector.

  5. The Sample and Fraction Manager (SFM) waits for the Inject Sync signal. (The Inject Sync signal is an SFM synchronization signal.) Set this signal to immediately if no waiting time is required. Alternatively, you can set the signal to an input port of the SFM if the instrument is to wait for a signal from the pump. (For information about the settings, refer to Installing Dionex Devices  HPLC Sample and Fraction Manager: Installation in the Administrator Help section.)

  6. The needle is moved into the injector.

  7. The sampler pauses for the time specified by PreInjDelay.

  8. The injection valve is moved into the Load position.

  9. The contents of the syringe are dispensed into the sample loop.

  10. The sampler pauses for the time specified by PostInjDelay.

  11. The injection valve is moved into the Inject position.

  12. The needle is moved out of the injector.

  13. The needle returns to the Home position.

For more information, refer to:

 Sample and Fraction Manager Commands

 Commands for Fraction Collection

 Overlapping Areas

 PGM File for Fraction Collection