Dionex Sample and Fraction Manager
Sample and Fraction Manager Commands

Apart from  General Device Commands, the Sample and Fraction Manager supports the following commands and properties (please note that display Filter level determines which commands and properties are displayed):

Status properties

 

Property

Value Range

Filter

Description

Busy

Busy/Idle

Expert

Indicates whether the device driver has not yet sent all commands or whether execution of a command is yet complete
(read-only).

CPUSerialNo

-

Expert

Indicates the CPU serial number of the connected sampler
(read-only).

DeviceState

Ready/Busy/
Init(Error)/Unknown

Normal

Indicates the current device status (read-only).

StatusDescription

-

Normal

Indicates the current action of the sampler. If no action is performed, Idle is displayed
(read-only).

Syringe

-

Normal

Indicates the installed syringe type (read-only).

 

Inject properties

 

Command

Value Range

Description

AirVolume

Depends on the volume of the installed syringe; indicated in µl.
Special value: Default*

Specifies the air volume that is drawn for the Inject and/or WashWithSample commands (in LC_Inject and LC_Overlap mode).

FillSpeed

Depends on the minimum and maximum speed of the installed syringe; indicated in µl/s.
Special value: Default*

Fill speed for the preparing fill strokes for the Inject and/or WashWithSample commands.

FillStrokes

0-99 or Default*

Number of preparing fill strokes for the Inject and/or WashWithSample commands (in LC_Inject and LC_Overlap mode).

FillVolume

Depends on the syringe volume; indicated in µl.
Special value: Default*

Fill volume for the single piston strokes (for the WashWithSample command).

InjectMode

LC_Inject
LC_Overlap

Injection mode. In sample programs, this value must be set prior to the Inject command.

Injector

List of injection objects for the respective arm of the installed Sample and Fraction Manager

Injector to be used for the Inject and/or WashWithSample commands (in LC_Inject and LC_Overlap mode). The setting is ignored in Custom injection mode.

InjectSpeed

Depends on the minimum and maximum speed of the installed syringe;
Special value: Default*

Syringe speed in µl/s for injections to the sample loop
(in LC_Inject and LC_Overlap mode).

NoOfPlungerStrokes

 

Indicates the number of strokes since the last reset (Value) and maximum allowed number (Max.) as reported by the device. (The property is read-only.)

NoOfInjects

 

Indicates the number of injections since the last reset (Value) and maximum allowed number (Max.) as reported by the device. (The property is read-only.)

Penetration

0 - maximum value of all installed tray types

Defines how deep the needle descends into the vial (parameter for Inject, PrepareThisSample, PrepareNextSample, WashWithSolvent).

Position

1 to maximum number of tubes allowed for the trays used in the current configuration.

Defines the absolute position via an index entry, which can be defined as <TrayName> <Tube>.

Note: When you select a different position, the Tube, Tray, and TrayName properties are updated automatically.

PostInjectDelay

0 - 99000 ms or Default*

Time between the injection and the removing of the needle from the injector (in LC_Inject and LC_Overlap mode).

PreInjectDelay

0 - 99000 ms or Default*

Time between inserting the needle in the injector and injecting the liquid into the sample loop (in LC_Inject mode).

PullupDelay

0 - 10000 ms or Default*

Indicates the delay time between drawing the liquid into the syringe and dispensing the volume that is not required (for the WashWithSample command).

Tray

 

Tray used (read-only; determined via the Position property).

 

TrayName

String

Indicates the name of the current tray

Note: If you select a different tray, the Tray property is updated automatically.

Tube

 

Number of the current tube in the tray (read-only; determined via the Position property)

 

*  Note: 

For the Default parameter, the default value set on the respective device is used.

 

Command for the analytical SFM

 

Command

Description

CloseStack

Closes all stacks that might be open. This might be useful when fraction collection is finished.

 

Commands for the LC_Overlap mode

 

Command

Description

PrepareNextSample

The autosampler fills the sample loop with the next sample, observing the parameters defined in the PGM file for that sample (Volume, Position, Tray, AirVolume, FillSpeed, FillStrokes, and PullupDelay). The syringe then returns to the Park position.

This command is ignored for InjectModes other than LC_Overlap.

This command is available only after the Inject command in the PGM File.

PrepareThisSample

The autosampler fills the sample loop with the current sample, observing the parameters defined in the PGM File for the current sample (also, see PrepareNextSample). This command is available only before the Inject command in the PGM File.

 

For more information, refer to:

 Commands for Fraction Collection

 Tips for Injection

 Overlapping Areas

 PGM File for Fraction Collection