At peak:

Click the arrow to display the list of all peaks of the active chromatogram. Select a peak from the list to compare two or more chromatograms with reference to this peak.

Normalized time and Normalized signal determine how the chromatograms are displayed.

Exception: Leave the field empty for normalization of the chromatogram length. Two chromatograms with different run times will then be displayed with the same length (if Normalize Time = Off).

Normalize time:

Select Off if time normalization will not be performed.

Select SHIFT to overlay the peaks selected under At peak as to the time.

Select Stretch if you want to not only overlay the peaks selected under At peak as to the time, but to simultaneously stretch or compress the chromatograms in x-direction for uniform time scaling in all chromatograms. Thus, the distance between 0 and 1 min (or n minutes) is the same for all chromatograms.

Normalize signal

Select the Normalize signal check box to normalize the chromatograms as to the signal height of the peak selected under At peak. The peak has the same height in all chromatograms. All other peaks are enlarged or reduced accordingly.

Time:

Specify the offset from the active chromatogram in x-direction. Click the up and/or down arrow to set the value (in percent). Or else, directly type the value in the input field.

Signal:

Specify the offset from the active chromatogram in y-direction. Click the up and/or down arrow to set the value (in percent). Or else, directly type the value in the input field

Overlay with peak decoration:

Select this check box if the decoration settings such as peak names, peak delimiters, or the baseline should not only apply to the active chromatogram but also to all chromatograms.

 Tip:

Peak modifications are possible only in an active chromatogram.

Overlay with right signal axis:

Select this check box to include a second signal axis on the right-hand side of the chromatogram. This axis always refers to the chromatogram that was added last. The arrow next to the name of the respective chromatogram indicates the axis that belongs to the respective chromatogram.

Mirror chromatogram:

Select this check box to display a mirrored image of the chromatogram added last.

Overlay
(default)

Select Overlay to overlay the single chromatograms in one window. All options under Normalization, Offset, and Overlay are available for selection.

Stack

Select Stack to display the single chromatograms in separate plots. Each plot has its own signal axis that is optimized to the signal height. Only the Normalize Time options are available for selection.

Mixed

Select Mixed to display all chromatograms of the same detector type on the same plot. Chromatograms of different detectors are displayed in different plots. All options under Normalization, Offset, and Overlay are available for selection.

Extended fractionation

Select this option to enable the Extended Fractionation view. This view allows you to display all autopurification samples that belong together. The default setting is that all sample types are displayed:

• the original analytical sample and/or

• the preparative sample and/or

• the fractionated sample.

The individual samples are displayed in separate plots (see Stack view above).

Selecting this option also activates the Extended Fractionation and 2D Retention Map tab pages. Use these tab pages to define the settings for displaying autopurification sample types and channels, as well as 2D data of the fractionated samples.

 Tip:

If the current sample is not an autopurification sample, the chromatograms are displayed in the Overlay view (see above).