Spiked Sample

The sample matrix can considerably influence sample analysis, particularly in certain ion and gas chromatography applications. To account for this, a known amount of one or more substances is added to unknown samples. In this way, the concentration of these substances is increased by an exact known value. After spiking, the original (unknown) and the spiked sample are analyzed using the Standard Addition calibration mode. The unknown and the spiked samples must be separated and analyzed with the same chromatographic method.

Spiked samples in Chromeleon have the Type (Sample Type) Spiked, indicated by the symbol .

Unspiked samples have the type Unspiked, indicated by the symbol .

When analyzing several unknown samples, assign the unknown samples to the associated spiked samples in the Std. Add. Group column. In this column, the samples are assigned to a common standard addition group.

Dionex recommends always spiking unknown samples with the same amount of the same substances. In the Ref. Amount Set column, you can then assign the spiked samples to the same amount values in the Amount Table of the QNT Editor.

For more information, refer to How to …: Calibrating  Standard Addition.