Manual Re-Integration
Splitting Peaks

Sometimes only one peak is recorded when two substances elute approximately at the same time. It may happen that one peak is detected instead of two. This depends on the concentration ratio of the substances and the arrangement of the single peaks. You can then add a second peak below the existing one (also, refer to  Inserting or Deleting Peaks). The following commands are available:

Split Peak

Move the mouse pointer to the position where you want to split the existing peak. Select Split Peak on the context menu to split the peak into two main peaks. The perpendicular line splits the peak exactly at the pointer position.

 Note:

Splitting the peak does not change the baseline.

Shape Shoulder

Select Shape Shoulder on the context menu to exponentially skim a Peak Shoulder from the existing peak. First, move the pointer to the position for the peak start of the peak shoulder and select Shape Shoulder. The exponential-skimming algorithm automatically calculates the peak end for the peak shoulder.

 Note: 

The shoulder maximum is calculated relative to the baseline. Thus, it must not correspond to the highest signal value.

Dionex recommends using the Peak Shoulder Threshold to define shoulder shaping for the entire quantification method.

Use the Rider Threshold and Maximum Rider Ratio detection parameters to define the peaks that will be detected as rider peaks. For more information, refer to Integrating Chromatograms and Identifying Peaks  Defining Rider Peaks.