The QNT Editor
Function of the QNT Editor

The QNT Editor allows you to define the quantification method (also referred to as QNT Method or method). The quantification method defines parameters for identifying the peaks within a chromatogram (assigning substances) and for calculating the amount/concentration from the recorded peak areas (calibration).

Peak Identification

Chromeleon supports two ways for identifying detected peaks, i.e., for assigning substance names.

Peaks are usually identified by their Retention Times. If a peak elutes within a defined time window, it is assigned the corresponding name, calibration function, etc.

If many peaks elute in rapid succession or if their order is changed by varying chromatographic conditions (for example, pH value), identification by the UV spectrum or the Mass Spectrum is more reliable.

 Note:

Peak identification by UV spectra requires use of a Photodiode Array Detector, such as the Dionex UVD 340, PDA-100, or PDA-3000, and the appropriate software configuration.

For mass spectra acquisition, a Mass Spectrometer and the Xcalibur software are required in addition to the Chromeleon software.

Calibration

The peak table provides all information for the calibration. The Amount columns and the recorded peak areas are used to calculate the offset, slope, and curve parameters.

 Tip:

With each new calibration, the results are automatically recalculated for the report as the integration programs update the peak table with the calibration constants and the recorded integration values.

To save you from having to determine the retention time of each peak manually, Chromeleon peak tables can be generated automatically. (For more information, refer to Integrating Chromatograms and Identifying Peaks  Autogenerating the Peak Table.)