Location:

Report Variable (Peak Results)

Dimension:

Dimension of Amounts/ µl (e.g., µg/µl)

Default:

-

Related Variables:

Amount, Volume

Description:

The Concentration peak result variable describes the quotient of the calculated amount and the injection volume. The value thus specifies the amount of a substance contained in a micro liter (µl) of the injected volume.

Parameters:

Compute the amount against the original calibration curve with offset

When calculating the amount of the unspiked sample, Chromeleon uses the "original" calibration curve with offset. If this parameter is not selected, the "shifted" calibration curve that goes through the origin is used.

Do not subtract the reference amount for spiked samples

When calculating the amount of the spiked samples, the reference amount, that is the spiked amount, is not subtracted. Thus, the indicated amount is the total amount.

 Tips:

This variable is especially meaningful if you enter an absolute amount value, such as 15.00 µg, in the amount column of the peak table instead of a concentration value; for example, 1.0 µg/µl.

In this case, the value is divided by the injected volume, for example, 20 µl, which results in comparable absolute amount values that are always normalized to 1 µl; for instance, 15 µg/20 µl = 0.75 µg per µl.