ExWavelength

 

Instrument Type:

Fluorescence detector

Type:

Integer

Value Range:

Detector-dependent

Default:

Detector-dependent

Related Commands:

EmWavelength

Description:

The ExWavelength parameter sets the excitation wavelength. This is the wavelength required to induce a specific substance to fluoresce.

Function:

Fluorescence can be generated only with light that is of higher energy than the emitted light. That is why the selected excitation wavelength must be lower than the emission wavelength. As the excitation wavelength, select the highest absorption maximum for which the wavelength is below the emission wavelength. This allows the substance that will be analyzed to absorb as much irradiated energy as possible. Select the ExWavelength parameter to enter this wavelength.